Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Western Blot, Neutralization, Bioassay, Control, ELISA Detection, ELISA Development, Neutralization Control

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 97924
View all IgG Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IgG1 Fc region

Specificity

Detects chimeric proteins containing a human IgG Fc region in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IgG Fc Antibody

Detection of IgG1 Fc antibody in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP antibody by Flow Cytometry.

Detection of IgG1 Fc in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP by Flow Cytometry.

(A) Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7) bound to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP was stained with Mouse Anti-Human IgG Fc Monoclonal Antibody (Catalog # MAB110) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). (B) In the absence of rhB7-H1/PD-L1 Fc Chimera, HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP was stained with Mouse Anti-Human IgG Fc Monoclonal Antibody (Catalog # MAB110) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IgG Fc Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Recombinant Human B7‑H1/PD‑L1 Fc Chimera (Catalog # 156-B7) bound to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP

Western Blot

1 µg/mL
Sample: Recombinant Human IgG Fc region under non-reducing conditions only

Reviewed Applications

Read 4 reviews rated 4.8 using MAB110 in the following applications:

Flow Cytometry Panel Builder

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG

Human IgG Fc Antibody Monoclonal Mouse IgG1 Clone # 97924

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Human IgG Fc Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human IgG Fc Antibody

For research use only

Citations for Human IgG Fc Antibody

Customer Reviews for Human IgG Fc Antibody (4)

4.8 out of 5
4 Customer Ratings
5 Stars
75%
4 Stars
25%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human IgG Fc Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Cell Lysates
    Species: Human
    Verified Customer | Posted 01/06/2026
    I used this antibody to detect an Fc tagged protein in a binding assay for a new molecule. The molecule is biotinylated while the protein is only Fc tagged. The binding between the immobilized molecule and the Fc-tagged protein was detected with this antibody followed by a HRP-secondary antibody
    Human IgG Fc Antibody MAB110
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Human IgG Fc Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 07/01/2022
    Blocked membrane with 5% milk in TBS-T (0.1%) for 2h at RT Used 500ng/ml antibody in 5% milk overnight at 4C Used ECL system for detection
    Human IgG Fc Antibody MAB110
  • Human IgG Fc Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 Human Cell Line
    Species: Human
    Verified Customer | Posted 10/09/2021
    Human IgG Fc Antibody MAB110
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 22820181
    Species: Human
    Verified Customer | Posted 02/04/2015

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FAQs for Human IgG Fc Antibody

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  • Q: What is the light chain of the Human IgG Fc Antibody, Catalog #s MAB110 and FAB110, Clone # 97924?

    A: Catalog #s MAB110 and FAB110 have a kappa light chain.

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