Human IL-1 RI Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB269F-100
FAB269F-025
Detection of IL‑1 RI in HUVEC Human Cells by Flow Cytometry.
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Product Details
Citations (6)
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Reviews (1)

Human IL-1 RI Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-1 RI in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-1 RI
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL-1 RI antibody in HUVEC Human Cells antibody by Flow Cytometry. View Larger

Detection of IL‑1 RI in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human IL-1 RI Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB269F, filled histogram) or control antibody (Catalog # IC108F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-1 RI

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex.

An IL-1 receptor accessory protein that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.

References
  1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.
Long Name
Interleukin 1 Receptor I
Entrez Gene IDs
3554 (Human); 16177 (Mouse)
Alternate Names
CD121 antigen-like family member A; CD121a antigen; CD121a; IL-1 RI; IL1R; IL1R1; IL1RI; IL-1RI; IL1RT1; IL-1RT1; IL-1RT-1; Interleukin 1 Receptor 1; interleukin 1 receptor, type I; interleukin receptor 1; interleukin-1 receptor type 1; p80

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Citations for Human IL-1 RI Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Zinc transport from the endoplasmic reticulum to the cytoplasm via Zip7 is necessary for barrier dysfunction mediated by inflammatory signaling in RPE cells
    Authors: Y Xu, M Twarog, N Li, A Banks, J Schustak, Y Bao, Q Huang, QG Medley
    PLoS ONE, 2022-07-28;17(7):e0271656.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. TNF-alpha and IL-1beta sensitize human MSC for IFN-gamma signalling and enhance neutrophil recruitment
    Authors: A Hackel, A Aksamit, K Bruderek, S Lang, S Brandau
    Eur. J. Immunol., 2020-09-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Comparing the Effects of the mTOR Inhibitors Azithromycin and Rapamycin on In Vitro Expanded Regulatory T Cells
    Authors: M Bergström, M Müller, M Karlsson, H Scholz, NT Vethe, O Korsgren
    Cell Transplant, 2019-09-12;0(0):9636897198724.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Plasmacytoid dendritic cells promote HIV-1-induced group 3 innate lymphoid cell depletion.
    Authors: Zhang Z, Cheng L, Zhao J, Li G, Zhang L, Chen W, Nie W, Reszka-Blanco N, Wang F, Su L
    J Clin Invest, 2015-08-24;125(9):3692-703.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. IL-11 Induces Th17 Cell Responses in Patients with Early Relapsing-Remitting Multiple Sclerosis.
    Authors: Zhang X, Tao Y, Chopra M, Dujmovic-Basuroski I, Jin J, Tang Y, Drulovic J, Markovic-Plese S
    J Immunol, 2015-04-20;194(11):5139-49.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Regulating human Th17 cells via differential expression of IL-1 receptor.
    Authors: Lee WW, Kang SW, Choi J
    Blood, 2009-11-12;115(3):530-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human IL-1 RI Fluorescein-conjugated Antibody
By Anonymous on 10/20/2021
Application: Flow Sample Tested: Blood mononuclear cells (PBMCs) Species: Human