Human IL-1 RI Antibody AF269: R&D Systems

Human IL-1 RI Antibody

(7 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-1 RI in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-1 RI
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human IL‑1 RI
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Neutralization
    Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced growth inhibition in the A375s2 human melanoma cell line. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 0.25 ng/mL Recombinant Human IL‑1 beta /IL‑1F2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑1 RI in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were labeled with Human IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Growth Inhibition Induced by IL‑1 beta /IL‑1F2 and Neutralization by Human IL‑1 RI Antibody. Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) inhibits proliferation in the A375s2 human melanoma cell line in a dose-dependent manner (orange line). Growth inhibition elicited by Recombinant Human IL‑1 beta /IL‑1F2 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269). The ND50 is typically 0.5-2.0 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 RI

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex.

An IL-1 receptor accessory protein that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.

  • References:
    1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.
  • Long Name:
    Interleukin 1 Receptor I
  • Entrez Gene IDs:
    3554 (Human); 16177 (Mouse)
  • Alternate Names:
    CD121 antigen-like family member A; CD121a antigen; CD121a; IL-1 RI; IL1R; IL1R1; IL1RI; IL-1RI; IL1RT1; IL-1RT1; IL-1RT-1; Interleukin 1 Receptor 1; interleukin 1 receptor, type I; interleukin receptor 1; interleukin-1 receptor type 1; p80
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Expression and function of IL-1R8 (TIR8/SIGIRR), a regulatory member of the IL-1 receptor family in platelets
    Authors: Achille Anselmo
    Cardiovasc Res, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways.
    Authors: Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D
    J Immunol, 2015;195(6):2818-28.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  3. Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma.
    Authors: Hengartner N, Fiedler J, Schrezenmeier H, Huber-Lang M, Brenner R
    PLoS ONE, 2015;10(1):e0116772.
    Species: Human
    Sample Type: Whole Cells
    Application: Blocking
  4. IL-1beta promotes the differentiation of polyfunctional human CCR6+CXCR3+ Th1/17 cells that are specific for pathogenic and commensal microbes.
    Authors: Duhen T, Campbell D
    J Immunol, 2014;193(1):120-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta.
    Authors: Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F
    Nature, 2012;484(7395):514-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Interleukin 1 in oviductal tissues of viviparous, oviparous, and ovuliparous species of amphibians.
    Authors: Jantra S, Bigliardi E, Brizzi R, Ietta F, Bechi N, Paulesu L
    Biol. Reprod., 2007;76(6):1009-15.
    Species: Amphibian
    Sample Type: Tissue Homogenates
    Application: WB
  7. Chorionic gonadotropin down-regulates the expression of the decoy inhibitory interleukin 1 receptor type II in human endometrial epithelial cells.
    Authors: Herrmann-Lavoie C, Rao CV, Akoum A
    Endocrinology, 2007;148(11):5377-84.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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Average Rating: 4 (Based on 1 review)

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ELISA Human IL-1 RI Antibody AF269
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 ELISA Human Anonymous 11/09/2017
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ELISA Human IL-1 RI Antibody AF269
ELISA: Human IL-1 RI Antibody [AF269]

Summary

ApplicationELISA
Sample TestedSerum and Plasma,Cell culture supernatant
SpeciesHuman

Other Experimental Details

Other Experimental DetailsThis polyclonal antibody (AF269) was used as both capture and detection for a sandwich assay for sIL-1R1. The immunoassay standard used was recombinant IL-1R1, 269-1R.

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