Detects human IL-1 RI in direct ELISAs and Western blots.
Polyclonal Goat IgG
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-1 RI
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human IL‑1 RI
0.25 µg/106 cells
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced growth inhibition in the A375s2 human melanoma cell line. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 0.25 ng/mL Recombinant Human IL‑1 beta /IL‑1F2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of IL‑1 RI in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were labeled with Human IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Growth Inhibition Induced by IL‑1 beta /IL‑1F2 and Neutralization by Human IL‑1 RI Antibody. Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) inhibits proliferation in the A375s2 human melanoma cell line in a dose-dependent manner (orange line). Growth inhibition elicited by Recombinant Human IL‑1 beta /IL‑1F2 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269). The ND50 is typically 0.5-2.0 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 RI
Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex.
An IL-1 receptor accessory protein that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.
Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.
Interleukin 1 Receptor I
Entrez Gene IDs:
3554 (Human); 16177 (Mouse)
CD121 antigen-like family member A; CD121a antigen; CD121a; IL-1 RI; IL1R; IL1R1; IL1RI; IL-1RI; IL1RT1; IL-1RT1; IL-1RT-1; Interleukin 1 Receptor 1; interleukin 1 receptor, type I; interleukin receptor 1; interleukin-1 receptor type 1; p80
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