Human IL-10 Quantikine ELISA Kit

  (102 citations)
(6 Reviews)
    
Datasheet / CoA / SDS
Product Details
Assay Procedure
Citations (102)
FAQs
Supplemental Products
Reviews
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    3.5 hours or 4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL)
  • Sensitivity
    3.9 pg/mL
  • Assay Range
    7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human IL-10
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC01-1 , Quantikine Immunoassay Control Group 1 - Please Inquire
Product Summary
The Quantikine Human IL-10 Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-10 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human IL-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-10 and does not cross-react with viral IL-10. Results obtained using natural human IL-10 showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate this kit can be used to determine relative mass values for natural human IL-10.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 24.4 79.6 223 23.6 74.2 227
Standard Deviation 1.6 3.9 5.5 1.8 4.9 12.7
CV% 6.6 4.9 2.5 7.6 6.6 5.6

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 23.9 76.9 231 23.2 75.1 228
Standard Deviation 1.2 3.3 3.9 1.7 5.6 13.4
CV% 5 4.3 1.7 7.3 7.5 5.9

Recovery

The recovery of human IL-10 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 98 88-110
Citrate Plasma (n=5) 96 87-108
EDTA Plasma (n=5) 92 75-105
Heparin Plasma (n=5) 94 84-105
Serum (n=5) 100 92-110
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of IL-10 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 IL-10 [HRP]
Product Datasheets

Certificate of Analysis Lookup

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-10
Interleukin-10 functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.
  • Long Name:
    Interleukin 10
  • Entrez Gene IDs:
    3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
  • Alternate Names:
    CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, working standards, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent for Serum & Plasma Samples Only
  4.   For Serum & Plasma Samples Only:  Add 50 µL of Assay Diluent to each well.

  5. 200 µL Standard, control, or sample
  6.   Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well, and cover with a new plate sealer.
  10.   For Serum & Plasma Samples:  Incubate at room temperature for 2 hours.
    For Cell Culture Supernate Samples:  Incubate at room temperature for 1 hour.
  11.   Aspirate and wash 4 times.

  12. 200 µL Substrate Solution
  13.   Add 200 µL Substrate Solution to each well. PROTECT FROM LIGHT.
  14. For Serum & Plasma Samples:  Incubate at room temperature for 30 minutes.
    For Cell Culture Supernate Samples:  Incubate at room temperature for 20 minutes.

  15. 50 µL Stop Solution
  16. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

102 Citations: Showing 1 - 10
Filter your results:

Species
Sample Type
  1. Stress-Dose Corticosteroid Versus Placebo in Neonatal Cardiac Operations: A Randomized Controlled Trial
    Authors: PK Suominen, J Keski-Nisu, T Ojala, P Rautiainen, T Jahnukaine, J Hästbacka, PJ Neuvonen, O Pitkänen, J Niemelä, A Kaskinen, J Salminen, R Lapatto
    Ann. Thorac. Surg., 2017;0(0):.
    Species: Human
    Sample Type: Plasma
  2. The Effect of Simulated Flash-Heat Pasteurization on Immune Components of Human Milk
    Authors: B Daniels, S Schmidt, T King, K Israel-Bal, K Amundson M, A Coutsoudis
    Nutrients, 2017;9(2):.
    Species: Human
    Sample Type: Milk
  3. An in vivo assessment of the cholesterol-lowering efficacy of Lactobacillus plantarum ECGC 13110402 in normal to mildly hypercholesterolaemic adults
    Authors: A Costabile, I Buttarazzi, S Kolida, S Quercia, J Baldini, JR Swann, P Brigidi, GR Gibson
    PLoS ONE, 2017;12(12):e0187964.
    Species: Human
    Sample Type: Serum
  4. The in vitro and ex vivo effect of Auranta 3001 in preventing Cryptosporidium hominis and Cryptosporidium parvum infection
    Authors: A Ch Stratak, F Sima, P Ward, M Linton, C Kelly, L Pinkerton, L Stef, I Pet, T Iancu, G Pircalabio, N Corcionivo
    Gut Pathog, 2017;9(0):49.
    Species: Bovine
    Sample Type: Cell Culture Supernates
  5. Regulated Polarization of Tumor-Associated Macrophages by miR-145 via Colorectal Cancer-Derived Extracellular Vesicles
    Authors: H Shinohara, Y Kuranaga, M Kumazaki, N Sugito, Y Yoshikawa, T Takai, K Taniguchi, Y Ito, Y Akao
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Type: Cell Culture Supernates
  6. Immunological and Inflammatory Impact of Non-Intubated Lung Metastasectomy
    Authors: TC Mineo, F Sellitri, G Vanni, FT Gallina, V Ambrogi
    Int J Mol Sci, 2017;18(7):.
    Species: Human
    Sample Type: Cell Culture Supernates
  7. Interleukin-1? levels predict long-term mortality and need for heart transplantation in ambulatory patients affected by idiopathic dilated cardiomyopathy
    Authors: A Aleksova, AP Beltrami, C Carriere, G Barbati, P Lesizza, M Perrieri-M, M Isola, P Gentile, E Salvioni, T Not, P Agostoni, G Sinagra
    Oncotarget, 2017;8(15):25131-25140.
    Species: Human
    Sample Type: Plasma
  8. Expression of hemoglobin-? and ? subunits in human vaginal epithelial cells and their functional significance
    Authors: D Saha, S Koli, M Patgaonkar, KV Reddy
    PLoS ONE, 2017;12(2):e0171084.
    Species: Human
    Sample Type: Cell Culture Supernates
  9. Serum and peritoneal fluid concentrations of soluble human leukocyte antigen, tumor necrosis factor alpha and interleukin 10 in patients with selected ovarian pathologies
    Authors: O Sipak-Szmi, P W?odarski, E Ronin-Walk, A Niedzielsk, B Karakiewic, S S?uczanows, M Laszczy?sk, W Malinowski
    J Ovarian Res, 2017;10(1):25.
    Species: Human
    Sample Type: Serum
  10. PU.1 serves a critical role in the innate defense against Aspergillus�fumigatus via dendritic cell-associated C-type lectin receptor-1 and toll-like receptors-2 and 4 in THP-1-derived macrophages
    Authors: C Liu, M Wang, W Sun, F Cai, S Geng, X Su, Y Shi
    Mol Med Rep, 2017;0(0):.
    Species: Human
    Sample Type: Cell Culture Supernates
  11. Anti-aging effect and gene expression profiling of dung beetle glycosaminoglycan in aged rats
    Authors: MY Ahn, BJ Kim, HJ Kim, JS Hwang, YS Jung, KK Park
    Biomater Res, 2017;21(0):5.
    Species: Rat
    Sample Type: Serum
  12. Simple Plex() : A Novel Multi-Analyte, Automated Microfluidic Immunoassay Platform for the Detection of Human and Mouse Cytokines and Chemokines.
    Authors: Aldo P, Marusov G, Svancara D, David J, Mor G
    Am J Reprod Immunol, 2016;75(6):678-93.
    Species: Human
    Sample Type: Plasma
  13. Altered Systemic Adipokines in Patients with Chronic Urticaria
    Authors: Hoang Kim Tu Trinh
    Int. Arch. Allergy Immunol, 2016;171(2):102-110.
    Species: Human
    Sample Type: Serum
  14. Analysis of VEGFA Variants and Changes in VEGF Levels Underscores the Contribution of VEGF to Polycystic Ovary Syndrome
    PLoS ONE, 2016;11(11):e0165636.
    Species: Human
    Sample Type: Plasma
  15. Association between Placental Lesions, Cytokines and Angiogenic Factors in Pregnant Women with Preeclampsia
    Authors: Ingrid C Weel
    PLoS ONE, 2016;11(6):e0157584.
    Species: Human
    Sample Type: Tissue Homogenates
  16. Inflammation-regulating factors in ascites as predictive biomarkers of drug resistance and progression-free survival in serous epithelial ovarian cancers.
    Authors: Lane D, Matte I, Garde-Granger P, Laplante C, Carignan A, Rancourt C, Piche A
    BMC Cancer, 2015;15(0):492.
    Species: Human
    Sample Type: Peritoneal Fluid
  17. WNT5A transforms intestinal CD8alphaalpha(+) IELs into an unconventional phenotype with pro-inflammatory features.
    Authors: Zhao D, Xu A, Dai Z, Peng J, Zhu M, Shen J, Zheng Q, Ran Z
    BMC Gastroenterol, 2015;15(0):173.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  18. The Association between Serum Cytokines and Damage to Large and Small Nerve Fibers in Diabetic Peripheral Neuropathy.
    Authors: Magrinelli F, Briani C, Romano M, Ruggero S, Toffanin E, Triolo G, Peter G, Praitano M, Lauriola M, Zanette G, Tamburin S
    J Diabetes Res, 2015;2015(0):547834.
    Species: Human
    Sample Type: Serum
  19. Association of candidate gene polymorphisms and TGF-beta/IL-10 levels with malaria in three regions of Cameroon: a case-control study.
    Authors: Apinjoh T, Anchang-Kimbi J, Njua-Yafi C, Ngwai A, Mugri R, Clark T, Rockett K, Kwiatkowski D, Achidi E
    Malar J, 2014;13(0):236.
    Species: Human
    Sample Type: Plasma
  20. Natural history of autoimmune lymphoproliferative syndrome associated with FAS gene mutations.
    Authors: Price S, Shaw P, Seitz A, Joshi G, Davis J, Niemela J, Perkins K, Hornung R, Folio L, Rosenberg P, Puck J, Hsu A, Lo B, Pittaluga S, Jaffe E, Fleisher T, Rao V, Lenardo M
    Blood, 2014;123(13):1989-99.
    Species: Human
    Sample Type: Serum
  21. Identification of a distinct glucocorticosteroid-insensitive pulmonary macrophage phenotype in patients with chronic obstructive pulmonary disease.
    Authors: Chana K, Fenwick P, Nicholson A, Barnes P, Donnelly L
    J Allergy Clin Immunol, 2014;133(1):207-16.e1-11.
    Species: Human
    Sample Type: Cell Culture Supernates
  22. Immune-inflammatory markers and arterial stiffness indexes in subjects with acute ischemic stroke with and without metabolic syndrome.
    Authors: Tuttolomondo A, Pecoraro R, Di Raimondo D, Di Sciacca R, Canino B, Arnao V, Butta C, Della Corte V, Maida C, Licata G, Pinto A
    Diabetol Metab Syndr, 2014;6(1):28.
    Species: Human
    Sample Type: Plasma
  23. Mycobacteria bypass mucosal NF-kB signalling to induce an epithelial anti-inflammatory IL-22 and IL-10 response.
    Authors: Lutay N, Hakansson G, Alaridah N, Hallgren O, Westergren-Thorsson G, Godaly G
    PLoS ONE, 2014;9(1):e86466.
    Species: Human
    Sample Type: Cell Culture Supernates
  24. Live attenuated B. pertussis BPZE1 rescues the immune functions of Respiratory Syncytial virus infected human dendritic cells by promoting Th1/Th17 responses.
    Authors: Schiavoni I, Fedele G, Quattrini A, Bianco M, Schnoeller C, Openshaw P, Locht C, Ausiello C
    PLoS ONE, 2014;9(6):e100166.
    Species: Human
    Sample Type: Cell Culture Supernates
  25. Endocannabinoids affect innate immunity of Muller glia during HIV-1 Tat cytotoxicity.
    Authors: Krishnan G, Chatterjee N
    Mol Cell Neurosci, 2014;59(0):10-23.
    Species: Human
    Sample Type: Cell Culture Supernates
  26. Effects of pitavastatin versus atorvastatin on the peripheral endothelial progenitor cells and vascular endothelial growth factor in high-risk patients: a pilot prospective, double-blind, randomized study.
    Authors: Lin L, Huang C, Chen J, Wu T, Leu H, Huang P, Chang T, Lin S, Chen J
    Cardiovasc Diabetol, 2014;13(0):111.
    Species: Human
    Sample Type: Serum
  27. Expression and functional characterization of NOD2 in decidual stromal cells isolated during the first trimester of pregnancy.
    Authors: Zhang Y, Chen H, Sun C, Wang H, Liu M, Li Y, Nie X, Du M, Li D, Zhang J
    PLoS ONE, 2014;9(6):e99612.
    Species: Human
    Sample Type: Cell Culture Supernates
  28. Characterization of programmed death-1 homologue-1 (PD-1H) expression and function in normal and HIV infected individuals.
    Authors: Bharaj, Preeti, Chahar, Harendra, Alozie, Ogechika, Rodarte, Lizette, Bansal, Anju, Goepfert, Paul A, Dwivedi, Alok, Manjunath, N, Shankar, Premlata
    PLoS ONE, 2014;9(10):e109103.
    Species: Human
    Sample Type: Serum
  29. The impact of concomitant pulmonary infection on immune dysregulation in Pneumocystis jirovecii pneumonia.
    Authors: Chou C, Lin F, Tsai H, Chang S
    BMC Pulm Med, 2014;14(0):182.
    Species: Human
    Sample Type: BALF
  30. Anti-GD2 antibody 3F8 and barley-derived (1 --> 3),(1 --> 4)-beta--glucan: A Phase I study in patients with chemoresistant neuroblastoma.
    Authors: Modak S, Kushner B, Kramer K, Vickers A, Cheung I, Cheung N
    Oncoimmunology, 2013;2(3):e23402.
    Species: Human
    Sample Type: Serum
  31. Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia coli via a modified Sec-dependent transporter construct.
    Authors: Forster S, Brandt M, Mottok D, Zschuttig A, Zimmermann K, Blattner F, Gunzer F, Pohlmann C
    BMC Biotechnol, 2013;13(0):82.
    Species: Virus
    Sample Type: Cell Fraction
  32. Bile acids PKA-dependently induce a switch of the IL-10/IL-12 ratio and reduce proinflammatory capability of human macrophages.
    Authors: Haselow K, Bode J, Wammers M, Ehlting C, Keitel V, Kleinebrecht L, Schupp A, Haussinger D, Graf D
    J Leukoc Biol, 2013;94(6):1253-64.
    Species: Human
    Sample Type: Cell Culture Supernates
  33. CLEC5A is critical for dengue virus-induced inflammasome activation in human macrophages.
    Authors: Wu M, Chen S, Yang A, Lin W, Lin Y, Chen N, Tsai I, Li L, Hsieh S
    Blood, 2013;121(1):95-106.
    Species: Human
    Sample Type: Cell Culture Supernates
  34. Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma.
    Authors: Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbe C, Caillat-Zucman S
    PLoS Pathog., 2012;8(1):e1002486.
    Species: Human
    Sample Type: Cell Culture Supernates
  35. Hypoxia potentiates glioma-mediated immunosuppression.
    Authors: Wei J, Wu A, Kong LY, Wang Y, Fuller G, Fokt I, Melillo G, Priebe W, Heimberger AB
    PLoS ONE, 2012;6(1):e16195.
    Species: Human
    Sample Type: Cell Culture Supernates
  36. Effects of TLR agonists on maturation and function of 3-day dendritic cells from AML patients in complete remission.
    Authors: Beck B, Dorfel D, Lichtenegger FS, Geiger C, Lindner L, Merk M, Schendel DJ, Subklewe M
    J Transl Med, 2011;9(0):151.
    Species: Human
    Sample Type: Cell Culture Supernates
  37. IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
    Authors: Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
    Clin. Cancer Res., 2011;17(24):7529-38.
    Species: Human
    Sample Type: Serum
  38. HDAC inhibition by LBH589 affects the phenotype and function of human myeloid dendritic cells.
    Authors: Song W, Tai YT, Tian Z, Hideshima T, Chauhan D, Nanjappa P, Exley MA, Anderson KC, Munshi NC
    Leukemia, 2011;25(1):161-8.
    Species: Human
    Sample Type: Cell Culture Supernates
  39. Presence of IL-5 protein and IgE antibodies to staphylococcal enterotoxins in nasal polyps is associated with comorbid asthma.
    Authors: Bachert C, Zhang N, Holtappels G, De Lobel L, Van Cauwenberge P, Liu S, Lin P, Bousquet J, Van Steen K
    J. Allergy Clin. Immunol., 2010;126(5):962.
  40. Involvement of regulatory T cells in the immunosuppression characteristic of patients with paracoccidioidomycosis.
    Authors: Ferreira MC, de Oliveira RT, da Silva RM, Blotta MH, Mamoni RL
    Infect. Immun., 2010;78(10):4392-401.
    Species: Human
    Sample Type: Cell Culture Supernates
  41. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
    Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Allergy Clin. Immunol., 2009;123(1):59-66.e4.
    Species: Human
    Sample Type: Cell Culture Supernates
  42. Cytokine secretion by human mesenchymal stem cells cocultured with damaged corneal epithelial cells.
    Authors: Oh JY, Kim MK, Shin MS, Wee WR, Lee JH
    Cytokine, 2009;46(1):100-3.
    Species: Human
    Sample Type: Cell Culture Supernates
  43. Normally occurring NKG2D+CD4+ T cells are immunosuppressive and inversely correlated with disease activity in juvenile-onset lupus.
    Authors: Dai Z, Turtle CJ, Booth GC, Riddell SR, Gooley TA, Stevens AM, Spies T, Groh V
    J. Exp. Med., 2009;206(4):793-805.
    Species: Human
    Sample Type: Cell Culture Supernates
  44. Direct and indirect impairment of human dendritic cell function by virulent Francisella tularensis Schu S4.
    Authors: Chase JC, Celli J, Bosio CM
    Infect. Immun., 2009;77(1):180-95.
    Species: Human
    Sample Type: Cell Culture Supernates
  45. HIV increases markers of cardiovascular risk: results from a randomized, treatment interruption trial.
    Authors: Calmy A, Gayet-Ageron A, Montecucco F, Nguyen A, Mach F, Burger F, Ubolyam S, Carr A, Ruxungtham K, Hirschel B, Ananworanich J,
    AIDS, 2009;23(8):929-39.
    Species: Human
    Sample Type: Plasma
  46. Immuno-inflammatory and thrombotic/fibrinolytic variables associated with acute ischemic stroke diagnosis.
    Authors: Tuttolomondo A, Pinto A, Corrao S, Di Raimondo D, Fernandez P, Di Sciacca R, Arnao V, Licata G
    Atherosclerosis, 2009;203(2):503-8.
    Species: Human
    Sample Type: Plasma
  47. Prostaglandin E2 regulates Th17 cell differentiation and function through cyclic AMP and EP2/EP4 receptor signaling.
    Authors: Boniface K, Bak-Jensen KS, Li Y, Blumenschein WM, McGeachy MJ, McClanahan TK, McKenzie BS, Kastelein RA, Cua DJ, de Waal Malefyt R
    J. Exp. Med., 2009;206(3):535-48.
    Species: Human
    Sample Type: Cell Culture Supernates
  48. Inflammatory interaction between LIGHT and proteinase-activated receptor-2 in endothelial cells: potential role in atherogenesis.
    Authors: Sandberg WJ, Halvorsen B, Yndestad A, Smith C, Otterdal K, Brosstad FR, Froland SS, Olofsson PS, Damas JK, Gullestad L, Hansson GK, Oie E, Aukrust P
    Circ. Res., 2009;104(1):60-8.
    Species: Human
    Sample Type: Cell Culture Supernates
  49. HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells.
    Authors: Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A
    J. Exp. Med., 2009;206(1):169-81.
    Species: Human
    Sample Type: Cell Culture Supernates
  50. Abnormal cytokine production by bone marrow stromal cells of multiple myeloma patients in response to RPMI8226 myeloma cells.
    Authors: Zdzisinska B, Bojarska-Junak A, Dmoszynska A, Kandefer-Szerszen M
    Arch. Immunol. Ther. Exp. (Warsz.), 2008;56(3):207-21.
    Species: Human
    Sample Type: Cell Culture Supernates
  51. In vivo transmigrated monocytes from patients with stable coronary artery disease have a reduced expression of CD11b.
    Authors: Paulsson JM, Dadfar E, Held C, Jacobson SH, Lundahl J
    Clin. Exp. Immunol., 2008;153(2):196-204.
    Species: Human
    Sample Type: Blister Fluid
  52. High levels and inflammatory effects of soluble CXC ligand 16 (CXCL16) in coronary artery disease: down-regulatory effects of statins.
    Authors: Smith C, Halvorsen B, Otterdal K, Waehre T, Yndestad A, Fevang B, Sandberg WJ, Breland UM, Froland SS, Oie E, Gullestad L, Damas JK, Aukrust P
    Cardiovasc. Res., 2008;79(1):195-203.
    Species: Human
    Sample Type: Cell Culture Supernates
  53. Mycobacterium bovis Bacillus Calmette-Guerin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells.
    Authors: Yokoi T, Amakawa R, Tanijiri T, Sugimoto H, Torii Y, Amuro H, Son Y, Tajima K, Liu YJ, Ito T, Fukuhara S
    Int. Immunol., 2008;20(10):1321-9.
    Species: Human
    Sample Type: Cell Culture Supernates
  54. Assessment of cervicovaginal cytokine levels following exposure to microbicide Nisin gel in rabbits.
    Authors: Aranha CC, Gupta SM, Reddy KV
    Cytokine, 2008;43(1):63-70.
    Species: Rabbit
    Sample Type: Vaginal Lavage
  55. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
    Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
    Eur. J. Immunol., 2008;38(4):1012-23.
    Species: Human
    Sample Type: Cell Culture Supernates
  56. Expression of human GITRL on myeloid dendritic cells enhances their immunostimulatory function but does not abrogate the suppressive effect of CD4+CD25+ regulatory T cells.
    Authors: Tuyaerts S, Van Meirvenne S, Bonehill A, Heirman C, Corthals J, Waldmann H, Breckpot K, Thielemans K, Aerts JL
    J. Leukoc. Biol., 2007;82(1):93-105.
    Species: Human
    Sample Type: Cell Culture Supernates
  57. Effects of darbepoetin-alpha on plasma pro-inflammatory cytokines, anti-inflammatory cytokine interleukin-10 and soluble Fas/Fas ligand system in anemic patients with chronic heart failure.
    Authors: Kourea K, Parissis JT, Farmakis D, Panou F, Paraskevaidis I, Venetsanou K, Filippatos G, Kremastinos DT
    Atherosclerosis, 2007;199(1):215-21.
    Species: Human
    Sample Type: Plasma
  58. Short communication: decreasing soluble CD30 and increasing IFN-gamma plasma levels are indicators of effective highly active antiretroviral therapy.
    Authors: Sadeghi M, Susal C, Daniel V, Naujokat C, Zimmermann R, Huth-Kuhne A, Opelz G
    AIDS Res. Hum. Retroviruses, 2007;23(7):886-90.
    Species: Human
    Sample Type: Plasma
  59. Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
    Authors: Lin YL, Liang YC, Chiang BL
    J. Leukoc. Biol., 2007;82(6):1473-80.
    Species: Human
    Sample Type: Cell Culture Supernates
  60. 1, 25 Dihydroxyvitamin D3 modulated cytokine response in pulmonary tuberculosis.
    Authors: Vidyarani M, Selvaraj P, Jawahar MS, Narayanan PR
    Cytokine, 2007;40(2):128-34.
    Species: Human
    Sample Type: Cell Culture Supernates
  61. Decreased plasma CXCL16/SR-PSOX concentration is associated with coronary artery disease.
    Authors: Sheikine Y, Bang CS, Nilsson L, Samnegard A, Hamsten A, Jonasson L, Eriksson P, Sirsjo A
    Atherosclerosis, 2006;188(2):462-6.
    Species: Human
    Sample Type: Serum
  62. Expression of IL-10 in patients with ovarian carcinoma.
    Authors: Mustea A, Konsgen D, Braicu EI, Pirvulescu C, Sun P, Sofroni D, Lichtenegger W, Sehouli J
    Anticancer Res., 2006;26(2):1715-8.
    Species: Human
    Sample Type: Serum
  63. The apoptotic-cell receptor CR3, but not alphavbeta5, is a regulator of human dendritic-cell immunostimulatory function.
    Authors: Skoberne M, Somersan S, Almodovar W, Truong T, Petrova K, Henson PM, Bhardwaj N
    Blood, 2006;108(3):947-55.
    Species: Human
    Sample Type: Cell Culture Supernates
  64. Influence of eotaxin 67G>A polymorphism on plasma eotaxin concentrations in myocardial infarction survivors and healthy controls.
    Authors: Sheikine Y, Olsen B, Gharizadeh B, Jatta K, Tornvall P, Ghaderi M
    Atherosclerosis, 2006;189(2):458-63.
    Species: Human
    Sample Type: Serum
  65. Induction of dendritic cell maturation by pertussis toxin and its B subunit differentially initiate Toll-like receptor 4-dependent signal transduction pathways.
    Authors: Wang ZY, Yang D, Chen Q, Leifer CA, Segal DM, Su SB, Caspi RR, Howard ZO, Oppenheim JJ
    Exp. Hematol., 2006;34(8):1115-24.
    Species: Human
    Sample Type: Cell Culture Supernates
  66. Interleukin-6 and vascular endothelial growth factor release by renal cell carcinoma cells impedes lymphocyte-dendritic cell cross-talk.
    Authors: Cabillic F, Bouet-Toussaint F, Toutirais O, Rioux-Leclercq N, Fergelot P, de La Pintiere CT, Genetet N, Patard JJ, Catros-Quemener V
    Clin. Exp. Immunol., 2006;146(3):518-23.
    Species: Human
    Sample Type: Cell Culture Supernates
  67. An investigation of auto-reactivity after head injury.
    Authors: Cox AL, Coles AJ, Nortje J, Bradley PG, Chatfield DA, Thompson SJ, Menon DK
    J. Neuroimmunol., 2006;174(1):180-6.
    Species: Human
    Sample Type: Cell Culture Supernates
  68. Bordetella pertussis inhibition of interleukin-12 (IL-12) p70 in human monocyte-derived dendritic cells blocks IL-12 p35 through adenylate cyclase toxin-dependent cyclic AMP induction.
    Authors: Spensieri F, Fedele G, Fazio C, Nasso M, Stefanelli P, Mastrantonio P, Ausiello CM
    Infect. Immun., 2006;74(5):2831-8.
    Species: Human
    Sample Type: Cell Culture Supernates
  69. Celecoxib up-regulates the expression of the zeta chain of T cell receptor complex in tumor-infiltrating lymphocytes in human cervical cancer.
    Authors: Ferrandina G, Ranelletti FO, Legge F, Salutari V, Martinelli E, Fattorossi A, Lorusso D, Zannoni G, Vellone V, Paglia A, Scambia G
    Clin. Cancer Res., 2006;12(7):2055-60.
    Species: Human
    Sample Type: Plasma
  70. Foxp3+CD4+CD25+ T cells control virus-specific memory T cells in chimpanzees that recovered from hepatitis C.
    Authors: Manigold T, Shin EC, Mizukoshi E, Mihalik K, Murthy KK, Rice CM, Piccirillo CA, Rehermann B
    Blood, 2006;107(11):4424-32.
    Species: Primate - Pan troglodytes (Chimpanzee)
    Sample Type: Cell Culture Supernates
  71. Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction.
    Authors: Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S
    Gut, 2006;56(2):243-52.
    Species: Human
    Sample Type: Cell Culture Supernates
  72. The potential of adiponectin in driving arthritis.
    Authors: Ehling A, Schaffler A, Herfarth H, Tarner IH, Anders S, Distler O, Paul G, Distler J, Gay S, Scholmerich J, Neumann E, Muller-Ladner U
    J. Immunol., 2006;176(7):4468-78.
    Species: Human
    Sample Type: Cell Culture Supernates
  73. No difference in the effects of clopidogrel and aspirin on inflammatory markers in patients with coronary heart disease.
    Authors: Solheim S, Pettersen AA, Arnesen H, Seljeflot I
    Thromb. Haemost., 2006;96(5):660-4.
    Species: Human
    Sample Type: Serum
  74. Airway inflammation in iron ore miners exposed to dust and diesel exhaust.
    Authors: Adelroth E, Hedlund U, Blomberg A, Helleday R, Ledin MC, Levin JO, Pourazar J, Sandstrom T, Jarvholm B
    Eur. Respir. J., 2006;27(4):714-9.
    Species: Human
    Sample Type: Sputum
  75. Experimental heatstroke in baboon: analysis of the systemic inflammatory response.
    Authors: Bouchama A, Ollivier V, Roberts G, Al Mohanna F, de Prost D, Eldali A, Saussereau E, El-Sayed R, Chollet-Martin S
    Shock, 2005;24(4):332-5.
    Species: Primate - Papio hamadryas (Hamadyras Baboon)
    Sample Type: Plasma
  76. Relationship between atopic status and nasal interleukin 10 and 11 levels in infants with respiratory syncytial virus bronchiolitis.
    Authors: Chung HL, Kim WT, Kim JK, Choi EJ, Lee JH, Lee GH, Kim SG
    Ann. Allergy Asthma Immunol., 2005;94(2):267-72.
    Species: Human
    Sample Type: Nasal Secretion
  77. Effects of the active metabolite of leflunomide, A77 1726, on cytokine release and the MAPK signalling pathway in human rheumatoid arthritis synoviocytes.
    Authors: Vergne-Salle&lt;/LastName&gt;&lt;F P&lt;/Initial, Vergne-Salle P, Leger DY, Bertin P, Treves R, Beneytout JL, Liagre B
    Cytokine, 2005;31(5):335-48.
    Species: Human
    Sample Type: Cell Culture Supernates
  78. Detection of interleukin 10 in cerebrospinal fluid of patients with subacute sclerosing panencephalitis.
    Authors: Mistchenko AS, Fornari MC, Viegas M, Barrero PR, Diez RA
    J. Neurovirol., 2005;11(1):66-9.
    Species: Human
    Sample Type: CSF
  79. Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
    Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
    Microbes Infect., 2005;7(3):427-36.
    Species: Human
    Sample Type: Serum
  80. Lack of phenotypic and functional impairment in dendritic cells from chimpanzees chronically infected with hepatitis C virus.
    Authors: Larsson M, Babcock E, Grakoui A, Shoukry N, Lauer G, Rice C, Walker C, Bhardwaj N
    J. Virol., 2004;78(12):6151-61.
    Species: Primate - Pan troglodytes (Chimpanzee)
    Sample Type: Cell Culture Supernates
  81. Human plasmacytoid dendritic cells activated by CpG oligodeoxynucleotides induce the generation of CD4+CD25+ regulatory T cells.
    Authors: Moseman EA, Liang X, Dawson AJ, Panoskaltsis-Mortari A, Krieg AM, Liu YJ, Blazar BR, Chen W
    J. Immunol., 2004;173(7):4433-42.
    Species: Human
    Sample Type: Cell Culture Supernates
  82. Antifungal immune reactivity in nasal polyposis.
    Authors: Pitzurra L, Bellocchio S, Nocentini A, Bonifazi P, Scardazza R, Gallucci L, Stracci F, Simoncelli C, Bistoni F, Romani L
    Infect. Immun., 2004;72(12):7275-81.
    Species: Human
    Sample Type: Nasal Lavage
  83. Effects of antiasthmatic agents on the functions of peripheral blood monocyte-derived dendritic cells from atopic patients.
    Authors: Saeki S, Matsuse H, Kondo Y, Machida I, Kawano T, Tomari S, Obase Y, Fukushima C, Kohno S
    J. Allergy Clin. Immunol., 2004;114(3):538-44.
    Species: Human
    Sample Type: Cell Culture Supernates
  84. Hepatocyte growth factor in polymorphonuclear leukocytes is increased in patients with systemic inflammatory response syndrome.
    Authors: Matsushima A, Ogura H, Koh T, Fujita K, Yoshiya K, Sumi Y, Hosotsubo H, Kuwagata Y, Tanaka H, Shimazu T, Sugimoto H
    J Trauma, 2004;56(2):259-64.
    Species: Human
    Sample Type: Plasma
  85. Inflammatory cytokine concentrations are elevated in seminal plasma of men with spinal cord injuries.
    Authors: Basu S, Aballa TC, Ferrell SM, Lynne CM, Brackett NL
    J. Androl., 2004;25(2):250-4.
    Species: Human
    Sample Type: Seminal Plasma
  86. High concentrations of circulating macrophage migration inhibitory factor in patients with severe blunt trauma: Is serum macrophage migration inhibitory factor concentration a valuable prognostic factor?
    Authors: Chuang CC, Hung CJ, Tsai MC, Chuang YC
    Crit. Care Med., 2004;32(3):734-9.
    Species: Human
    Sample Type: Serum
  87. Changes in CSF S100B and cytokine concentrations in early-phase severe traumatic brain injury.
    Authors: Hayakata T, Shiozaki T, Tasaki O, Ikegawa H, Inoue Y, Toshiyuki F, Hosotubo H, Kieko F, Yamashita T, Tanaka H, Shimazu T, Sugimoto H
    Shock, 2004;22(2):102-7.
    Species: Human
    Sample Type: Serum
  88. TGF-beta in human milk is associated with wheeze in infancy.
    Authors: Oddy WH, Halonen M, Martinez FD, Lohman IC, Stern DA, Kurzius-Spencer M, Guerra S, Wright AL
    J. Allergy Clin. Immunol., 2003;112(4):723-8.
    Species: Human
    Sample Type: Milk
  89. Urinary IL-2 assay for monitoring intravesical bacillus Calmette-Guerin response of superficial bladder cancer during induction course and maintenance therapy.
    Authors: Saint F, Kurth N, Maille P, Vordos D, Hoznek A, Soyeux P, Patard JJ, Abbou CC, Chopin DK
    Int. J. Cancer, 2003;107(3):434-40.
    Species: Human
    Sample Type: Urine
  90. Cytokine profile in Behcet's disease patients. Relationship with disease activity.
    Authors: Hamzaoui K, Hamzaoui A, Guemira F, Bessioud M, Hamza M, Ayed K
    Scand. J. Rheumatol., 2002;31(4):205-10.
    Species: Human
    Sample Type: Serum
  91. Skewing of cytotoxic activity and chemokine production, but not of chemokine receptor expression, in human type-1/-2 gamma delta T lymphocytes.
    Authors: D&amp;amp;apos;Ambrosio D
    Eur. J. Immunol., 2002;32(10):2934-43.
    Species: Human
    Sample Type: Cell Culture Supernates
  92. Cytokine pattern in blister fluid and sera of patients with pemphigus.
    Authors: Baroni A, Perfetto B, Ruocco E, Greco R, Criscuolo D, Ruocco V
    Dermatology (Basel), 2002;205(2):116-21.
    Species: Human
    Sample Type: Serum
  93. HIV-1 protein Vpr suppresses IL-12 production from human monocytes by enhancing glucocorticoid action: potential implications of Vpr coactivator activity for the innate and cellular immunity deficits observed in HIV-1 infection.
    Authors: Mirani M, Elenkov I, Volpi S, Hiroi N, Chrousos GP, Kino T
    J. Immunol., 2002;169(11):6361-8.
    Species: Human
    Sample Type: Cell Culture Supernates
  94. Intraperitoneal bispecific antibody (HEA125xOKT3) therapy inhibits malignant ascites production in advanced ovarian carcinoma.
    Authors: Marme A, Strauss G, Bastert G, Grischke EM, Moldenhauer G
    Int. J. Cancer, 2002;101(2):183-9.
    Species: Human
    Sample Type: Ascites Fluid
  95. Endogenously expressed nef uncouples cytokine and chemokine production from membrane phenotypic maturation in dendritic cells.
    Authors: Messmer D, Jacque JM, Santisteban C, Bristow C, Han SY, Villamide-Herrera L, Mehlhop E, Marx PA, Steinman RM, Gettie A, Pope M
    J. Immunol., 2002;169(8):4172-82.
    Species: Human
    Sample Type: Cell Culture Supernates
  96. Chimeric co-stimulatory molecules that selectively act through CD28 or CTLA-4 on human T cells.
    Authors: Lazetic S, Leong SR, Chang JC, Ong R, Dawes G, Punnonen J
    J. Biol. Chem., 2002;277(41):38660-8.
    Species: Human
    Sample Type: Cell Culture Supernates
  97. Interleukin-11 induces Th2 polarization of human CD4(+) T cells.
    Authors: Curti A, Ratta M, Corinti S, Girolomoni G, Ricci F, Tazzari P, Siena M, Grande A, Fogli M, Tura S, Lemoli RM
    Blood, 2001;97(9):2758-63.
    Species: Human
    Sample Type: Cell Culture Supernates
  98. Structural features of peptide analogs of human histocompatibility leukocyte antigen class I epitopes that are more potent and immunogenic than wild-type peptide.
    Authors: Tangri S, Ishioka GY, Huang X, Sidney J, Southwood S, Fikes J, Sette A
    J. Exp. Med., 2001;194(6):833-46.
    Species: Human
    Sample Type: Cell Culture Supernates
  99. Mature dendritic cells infiltrate the T cell-rich region of oral mucosa in chronic periodontitis: in situ, in vivo, and in vitro studies.
    Authors: Jotwani R, Palucka AK, Al-Quotub M, Nouri-Shirazi M, Kim J, Bell D, Banchereau J, Cutler CW
    J. Immunol., 2001;167(8):4693-700.
    Species: Human
    Sample Type: Gingival Crevicular Fluid (GCF)
  100. Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
    Authors: Kadowaki N, Antonenko S, Lau JY, Liu YJ
    J. Exp. Med., 2000;192(2):219-26.
    Species: Human
    Sample Type: Cell Culture Supernates
  101. Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGF-beta, PGE2, and PAF.
    Authors: Fadok, V A, Bratton, D L, Konowal, A, Freed, P W, Westcott, J Y, Henson, P M
    J Clin Invest, 1998;101(4):890-8.
    Species: Human
    Sample Type: Cell Culture Supernates
  102. Ex vivo adenoviral vector gene delivery results in decreased vector-associated inflammation pre- and post-lung transplantation in the pig.
    Authors: Yeung JC, Wagnetz D, Cypel M, Rubacha M, Koike T, Chun YM, Hu J, Waddell TK, Hwang DM, Liu M, Keshavjee S
    Mol. Ther., 212;20(6):1204-11.
    Species: Complex Species Category
    Sample Type: Plasma
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Average Rating: 4.8 (Based on 6 reviews)

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  Anonymous 03/30/2018
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Sample Tested mouse serum
 Human IL-10 Quantikine ELISA Kit D1000B Array D1000B
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 Human IL-10 Quantikine ELISA Kit D1000B
: Human IL-10 Quantikine ELISA Kit [D1000B].

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Other Experimental Details Used human synovial fluid samples with a dilution of 1 in 5 using DI water. Worked beautifully!
 Human IL-10 Quantikine ELISA Kit D1000B Array D1000B
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  Anonymous 04/24/2017
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 Human IL-10 Quantikine ELISA Kit D1000B
: Human IL-10 Quantikine ELISA Kit [D1000B].

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Other Experimental Details The Human IL-10 Quantikine ELISA kit is used for Diagnostic use.
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Sample Tested Serum and Plasma
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  Anonymous 08/03/2016
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 Human IL-10 Quantikine ELISA Kit D1000B
: Human IL-10 Quantikine ELISA Kit [D1000B].

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