• Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    3.5 hours or 4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL)
  • Sensitivity
    3.9 pg/mL
  • Assay Range
    7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human IL-10
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC01-1, Quantikine Immunoassay Control Group 1 - Please Inquire
Product Summary
The Quantikine Human IL-10 Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-10 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human IL-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-10 and does not cross-react with viral IL-10. Results obtained using natural human IL-10 showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate this kit can be used to determine relative mass values for natural human IL-10.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean24.479.622323.674.2227
Standard Deviation1.63.95.51.84.912.7
CV%6.64.92.57.66.65.6

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean23.976.923123.275.1228
Standard Deviation1.23.33.91.75.613.4
CV%54.31.77.37.55.9

Recovery

The recovery of human IL-10 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 98 88-110
Citrate Plasma (n=5) 96 87-108
EDTA Plasma (n=5) 92 75-105
Heparin Plasma (n=5) 94 84-105
Serum (n=5) 100 92-110
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of IL-10 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-10 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-10
Interleukin-10 functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.
    • Long Name
      Interleukin 10
    • Entrez Gene IDs
      3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral);
    • Alternate Names
      CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, working standards, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent for Serum & Plasma Samples Only
    4.   For Serum & Plasma Samples Only:  Add 50 µL of Assay Diluent to each well.

    5. 200 µL Standard, control, or sample
    6.   Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well, and cover with a new plate sealer.
    10.   For Serum & Plasma Samples:  Incubate at room temperature for 2 hours.
      For Cell Culture Supernate Samples:  Incubate at room temperature for 1 hour.
    11.   Aspirate and wash 4 times.

    12. 200 µL Substrate Solution
    13.   Add 200 µL Substrate Solution to each well. PROTECT FROM LIGHT.
    14. For Serum & Plasma Samples:  Incubate at room temperature for 30 minutes.
      For Cell Culture Supernate Samples:  Incubate at room temperature for 20 minutes.

    15. 50 µL Stop Solution
    16. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    97 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. Regulated Polarization of Tumor-Associated Macrophages by miR-145 via Colorectal Cancer-Derived Extracellular Vesicles
      Authors: H Shinohara, Y Kuranaga, M Kumazaki, N Sugito, Y Yoshikawa, T Takai, K Taniguchi, Y Ito, Y Akao
      J. Immunol., 2017;0(0):.
      Species: Human
      Sample Type: Cell Culture Supernates
    2. Immunological and Inflammatory Impact of Non-Intubated Lung Metastasectomy
      Authors: TC Mineo, F Sellitri, G Vanni, FT Gallina, V Ambrogi
      Int J Mol Sci, 2017;18(7):.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. Interleukin-1? levels predict long-term mortality and need for heart transplantation in ambulatory patients affected by idiopathic dilated cardiomyopathy
      Authors: A Aleksova, AP Beltrami, C Carriere, G Barbati, P Lesizza, M Perrieri-M, M Isola, P Gentile, E Salvioni, T Not, P Agostoni, G Sinagra
      Oncotarget, 2017;8(15):25131-25140.
      Species: Human
      Sample Type: Plasma
    4. Serum and peritoneal fluid concentrations of soluble human leukocyte antigen, tumor necrosis factor alpha and interleukin 10 in patients with selected ovarian pathologies
      Authors: O Sipak-Szmi, P W?odarski, E Ronin-Walk, A Niedzielsk, B Karakiewic, S S?uczanows, M Laszczy?sk, W Malinowski
      J Ovarian Res, 2017;10(1):25.
      Species: Human
      Sample Type: Serum
    5. PU.1 serves a critical role in the innate defense against Aspergillus�fumigatus via dendritic cell-associated C-type lectin receptor-1 and toll-like receptors-2 and 4 in THP-1-derived macrophages
      Authors: C Liu, M Wang, W Sun, F Cai, S Geng, X Su, Y Shi
      Mol Med Rep, 2017;0(0):.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. Anti-aging effect and gene expression profiling of dung beetle glycosaminoglycan in aged rats
      Authors: MY Ahn, BJ Kim, HJ Kim, JS Hwang, YS Jung, KK Park
      Biomater Res, 2017;21(0):5.
      Species: Rat
      Sample Type: Serum
    7. Expression of hemoglobin-? and ? subunits in human vaginal epithelial cells and their functional significance
      Authors: D Saha, S Koli, M Patgaonkar, KV Reddy
      PLoS ONE, 2017;12(2):e0171084.
      Species: Human
      Sample Type: Cell Culture Supernates
    8. Simple Plex() : A Novel Multi-Analyte, Automated Microfluidic Immunoassay Platform for the Detection of Human and Mouse Cytokines and Chemokines.
      Authors: Aldo P, Marusov G, Svancara D, David J, Mor G
      Am J Reprod Immunol, 2016;75(6):678-93.
      Species: Human
      Sample Type: Plasma
    9. Association between Placental Lesions, Cytokines and Angiogenic Factors in Pregnant Women with Preeclampsia
      Authors: Ingrid C Weel
      PLoS ONE, 2016;11(6):e0157584.
      Species: Human
      Sample Type: Tissue Homogenates
    10. Analysis of VEGFA Variants and Changes in VEGF Levels Underscores the Contribution of VEGF to Polycystic Ovary Syndrome
      PLoS ONE, 2016;11(11):e0165636.
      Species: Human
      Sample Type: Plasma
    11. WNT5A transforms intestinal CD8alphaalpha(+) IELs into an unconventional phenotype with pro-inflammatory features.
      Authors: Zhao D, Xu A, Dai Z, Peng J, Zhu M, Shen J, Zheng Q, Ran Z
      BMC Gastroenterol, 2015;15(0):173.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    12. The Association between Serum Cytokines and Damage to Large and Small Nerve Fibers in Diabetic Peripheral Neuropathy.
      Authors: Magrinelli F, Briani C, Romano M, Ruggero S, Toffanin E, Triolo G, Peter G, Praitano M, Lauriola M, Zanette G, Tamburin S
      J Diabetes Res, 2015;2015(0):547834.
      Species: Human
      Sample Type: Serum
    13. Inflammation-regulating factors in ascites as predictive biomarkers of drug resistance and progression-free survival in serous epithelial ovarian cancers.
      Authors: Lane D, Matte I, Garde-Granger P, Laplante C, Carignan A, Rancourt C, Piche A
      BMC Cancer, 2015;15(0):492.
      Species: Human
      Sample Type: Peritoneal Fluid
    14. Association of candidate gene polymorphisms and TGF-beta/IL-10 levels with malaria in three regions of Cameroon: a case-control study.
      Authors: Apinjoh T, Anchang-Kimbi J, Njua-Yafi C, Ngwai A, Mugri R, Clark T, Rockett K, Kwiatkowski D, Achidi E
      Malar J, 2014;13(0):236.
      Species: Human
      Sample Type: Plasma
    15. The impact of concomitant pulmonary infection on immune dysregulation in Pneumocystis jirovecii pneumonia.
      Authors: Chou C, Lin F, Tsai H, Chang S
      BMC Pulm Med, 2014;14(0):182.
      Species: Human
      Sample Type: BALF
    16. Natural history of autoimmune lymphoproliferative syndrome associated with FAS gene mutations.
      Authors: Price S, Shaw P, Seitz A, Joshi G, Davis J, Niemela J, Perkins K, Hornung R, Folio L, Rosenberg P, Puck J, Hsu A, Lo B, Pittaluga S, Jaffe E, Fleisher T, Rao V, Lenardo M
      Blood, 2014;123(13):1989-99.
      Species: Human
      Sample Type: Serum
    17. Identification of a distinct glucocorticosteroid-insensitive pulmonary macrophage phenotype in patients with chronic obstructive pulmonary disease.
      Authors: Chana K, Fenwick P, Nicholson A, Barnes P, Donnelly L
      J Allergy Clin Immunol, 2014;133(1):207-16.e1-11.
      Species: Human
      Sample Type: Cell Culture Supernates
    18. Mycobacteria bypass mucosal NF-kB signalling to induce an epithelial anti-inflammatory IL-22 and IL-10 response.
      Authors: Lutay N, Hakansson G, Alaridah N, Hallgren O, Westergren-Thorsson G, Godaly G
      PLoS ONE, 2014;9(1):e86466.
      Species: Human
      Sample Type: Cell Culture Supernates
    19. Immune-inflammatory markers and arterial stiffness indexes in subjects with acute ischemic stroke with and without metabolic syndrome.
      Authors: Tuttolomondo A, Pecoraro R, Di Raimondo D, Di Sciacca R, Canino B, Arnao V, Butta C, Della Corte V, Maida C, Licata G, Pinto A
      Diabetol Metab Syndr, 2014;6(1):28.
      Species: Human
      Sample Type: Plasma
    20. Expression and functional characterization of NOD2 in decidual stromal cells isolated during the first trimester of pregnancy.
      Authors: Zhang Y, Chen H, Sun C, Wang H, Liu M, Li Y, Nie X, Du M, Li D, Zhang J
      PLoS ONE, 2014;9(6):e99612.
      Species: Human
      Sample Type: Cell Culture Supernates
    21. Live attenuated B. pertussis BPZE1 rescues the immune functions of Respiratory Syncytial virus infected human dendritic cells by promoting Th1/Th17 responses.
      Authors: Schiavoni I, Fedele G, Quattrini A, Bianco M, Schnoeller C, Openshaw P, Locht C, Ausiello C
      PLoS ONE, 2014;9(6):e100166.
      Species: Human
      Sample Type: Cell Culture Supernates
    22. Effects of pitavastatin versus atorvastatin on the peripheral endothelial progenitor cells and vascular endothelial growth factor in high-risk patients: a pilot prospective, double-blind, randomized study.
      Authors: Lin L, Huang C, Chen J, Wu T, Leu H, Huang P, Chang T, Lin S, Chen J
      Cardiovasc Diabetol, 2014;13(0):111.
      Species: Human
      Sample Type: Serum
    23. Characterization of programmed death-1 homologue-1 (PD-1H) expression and function in normal and HIV infected individuals.
      Authors: Bharaj, Preeti, Chahar, Harendra, Alozie, Ogechika, Rodarte, Lizette, Bansal, Anju, Goepfert, Paul A, Dwivedi, Alok, Manjunath, N, Shankar, Premlata
      PLoS ONE, 2014;9(10):e109103.
      Species: Human
      Sample Type: Serum
    24. Endocannabinoids affect innate immunity of Muller glia during HIV-1 Tat cytotoxicity.
      Authors: Krishnan G, Chatterjee N
      Mol Cell Neurosci, 2014;59(0):10-23.
      Species: Human
      Sample Type: Cell Culture Supernates
    25. Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia coli via a modified Sec-dependent transporter construct.
      Authors: Forster S, Brandt M, Mottok D, Zschuttig A, Zimmermann K, Blattner F, Gunzer F, Pohlmann C
      BMC Biotechnol, 2013;13(0):82.
      Species: Virus
      Sample Type: Cell Fraction
    26. CLEC5A is critical for dengue virus-induced inflammasome activation in human macrophages.
      Authors: Wu M, Chen S, Yang A, Lin W, Lin Y, Chen N, Tsai I, Li L, Hsieh S
      Blood, 2013;121(1):95-106.
      Species: Human
      Sample Type: Cell Culture Supernates
    27. Anti-GD2 antibody 3F8 and barley-derived (1 --> 3),(1 --> 4)-beta--glucan: A Phase I study in patients with chemoresistant neuroblastoma.
      Authors: Modak S, Kushner B, Kramer K, Vickers A, Cheung I, Cheung N
      Oncoimmunology, 2013;2(3):e23402.
      Species: Human
      Sample Type: Serum
    28. Bile acids PKA-dependently induce a switch of the IL-10/IL-12 ratio and reduce proinflammatory capability of human macrophages.
      Authors: Haselow K, Bode J, Wammers M, Ehlting C, Keitel V, Kleinebrecht L, Schupp A, Haussinger D, Graf D
      J Leukoc Biol, 2013;94(6):1253-64.
      Species: Human
      Sample Type: Cell Culture Supernates
    29. Hypoxia potentiates glioma-mediated immunosuppression.
      Authors: Wei J, Wu A, Kong LY, Wang Y, Fuller G, Fokt I, Melillo G, Priebe W, Heimberger AB
      PLoS ONE, 2012;6(1):e16195.
      Species: Human
      Sample Type: Cell Culture Supernates
    30. Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma.
      Authors: Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbe C, Caillat-Zucman S
      PLoS Pathog., 2012;8(1):e1002486.
      Species: Human
      Sample Type: Cell Culture Supernates
    31. HDAC inhibition by LBH589 affects the phenotype and function of human myeloid dendritic cells.
      Authors: Song W, Tai YT, Tian Z, Hideshima T, Chauhan D, Nanjappa P, Exley MA, Anderson KC, Munshi NC
      Leukemia, 2011;25(1):161-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    32. Effects of TLR agonists on maturation and function of 3-day dendritic cells from AML patients in complete remission.
      Authors: Beck B, Dorfel D, Lichtenegger FS, Geiger C, Lindner L, Merk M, Schendel DJ, Subklewe M
      J Transl Med, 2011;9(0):151.
      Species: Human
      Sample Type: Cell Culture Supernates
    33. IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
      Authors: Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
      Clin. Cancer Res., 2011;17(24):7529-38.
      Species: Human
      Sample Type: Serum
    34. Involvement of regulatory T cells in the immunosuppression characteristic of patients with paracoccidioidomycosis.
      Authors: Ferreira MC, de Oliveira RT, da Silva RM, Blotta MH, Mamoni RL
      Infect. Immun., 2010;78(10):4392-401.
      Species: Human
      Sample Type: Cell Culture Supernates
    35. Presence of IL-5 protein and IgE antibodies to staphylococcal enterotoxins in nasal polyps is associated with comorbid asthma.
      Authors: Bachert C, Zhang N, Holtappels G, De Lobel L, Van Cauwenberge P, Liu S, Lin P, Bousquet J, Van Steen K
      J. Allergy Clin. Immunol., 2010;126(5):962.
    36. HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells.
      Authors: Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A
      J. Exp. Med., 2009;206(1):169-81.
      Species: Human
      Sample Type: Cell Culture Supernates
    37. Immuno-inflammatory and thrombotic/fibrinolytic variables associated with acute ischemic stroke diagnosis.
      Authors: Tuttolomondo A, Pinto A, Corrao S, Di Raimondo D, Fernandez P, Di Sciacca R, Arnao V, Licata G
      Atherosclerosis, 2009;203(2):503-8.
      Species: Human
      Sample Type: Plasma
    38. Cytokine secretion by human mesenchymal stem cells cocultured with damaged corneal epithelial cells.
      Authors: Oh JY, Kim MK, Shin MS, Wee WR, Lee JH
      Cytokine, 2009;46(1):100-3.
      Species: Human
      Sample Type: Cell Culture Supernates
    39. HIV increases markers of cardiovascular risk: results from a randomized, treatment interruption trial.
      Authors: Calmy A, Gayet-Ageron A, Montecucco F, Nguyen A, Mach F, Burger F, Ubolyam S, Carr A, Ruxungtham K, Hirschel B, Ananworanich J,
      AIDS, 2009;23(8):929-39.
      Species: Human
      Sample Type: Plasma
    40. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
      Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
      J. Allergy Clin. Immunol., 2009;123(1):59-66.e4.
      Species: Human
      Sample Type: Cell Culture Supernates
    41. Inflammatory interaction between LIGHT and proteinase-activated receptor-2 in endothelial cells: potential role in atherogenesis.
      Authors: Sandberg WJ, Halvorsen B, Yndestad A, Smith C, Otterdal K, Brosstad FR, Froland SS, Olofsson PS, Damas JK, Gullestad L, Hansson GK, Oie E, Aukrust P
      Circ. Res., 2009;104(1):60-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    42. Direct and indirect impairment of human dendritic cell function by virulent Francisella tularensis Schu S4.
      Authors: Chase JC, Celli J, Bosio CM
      Infect. Immun., 2009;77(1):180-95.
      Species: Human
      Sample Type: Cell Culture Supernates
    43. Normally occurring NKG2D+CD4+ T cells are immunosuppressive and inversely correlated with disease activity in juvenile-onset lupus.
      Authors: Dai Z, Turtle CJ, Booth GC, Riddell SR, Gooley TA, Stevens AM, Spies T, Groh V
      J. Exp. Med., 2009;206(4):793-805.
      Species: Human
      Sample Type: Cell Culture Supernates
    44. Prostaglandin E2 regulates Th17 cell differentiation and function through cyclic AMP and EP2/EP4 receptor signaling.
      Authors: Boniface K, Bak-Jensen KS, Li Y, Blumenschein WM, McGeachy MJ, McClanahan TK, McKenzie BS, Kastelein RA, Cua DJ, de Waal Malefyt R
      J. Exp. Med., 2009;206(3):535-48.
      Species: Human
      Sample Type: Cell Culture Supernates
    45. In vivo transmigrated monocytes from patients with stable coronary artery disease have a reduced expression of CD11b.
      Authors: Paulsson JM, Dadfar E, Held C, Jacobson SH, Lundahl J
      Clin. Exp. Immunol., 2008;153(2):196-204.
      Species: Human
      Sample Type: Blister Fluid
    46. Mycobacterium bovis Bacillus Calmette-Guerin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells.
      Authors: Yokoi T, Amakawa R, Tanijiri T, Sugimoto H, Torii Y, Amuro H, Son Y, Tajima K, Liu YJ, Ito T, Fukuhara S
      Int. Immunol., 2008;20(10):1321-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    47. Abnormal cytokine production by bone marrow stromal cells of multiple myeloma patients in response to RPMI8226 myeloma cells.
      Authors: Zdzisinska B, Bojarska-Junak A, Dmoszynska A, Kandefer-Szerszen M
      Arch. Immunol. Ther. Exp. (Warsz.), 2008;56(3):207-21.
      Species: Human
      Sample Type: Cell Culture Supernates
    48. Assessment of cervicovaginal cytokine levels following exposure to microbicide Nisin gel in rabbits.
      Authors: Aranha CC, Gupta SM, Reddy KV
      Cytokine, 2008;43(1):63-70.
      Species: Rabbit
      Sample Type: Vaginal Lavage
    49. High levels and inflammatory effects of soluble CXC ligand 16 (CXCL16) in coronary artery disease: down-regulatory effects of statins.
      Authors: Smith C, Halvorsen B, Otterdal K, Waehre T, Yndestad A, Fevang B, Sandberg WJ, Breland UM, Froland SS, Oie E, Gullestad L, Damas JK, Aukrust P
      Cardiovasc. Res., 2008;79(1):195-203.
      Species: Human
      Sample Type: Cell Culture Supernates
    50. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
      Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
      Eur. J. Immunol., 2008;38(4):1012-23.
      Species: Human
      Sample Type: Cell Culture Supernates
    51. Effects of darbepoetin-alpha on plasma pro-inflammatory cytokines, anti-inflammatory cytokine interleukin-10 and soluble Fas/Fas ligand system in anemic patients with chronic heart failure.
      Authors: Kourea K, Parissis JT, Farmakis D, Panou F, Paraskevaidis I, Venetsanou K, Filippatos G, Kremastinos DT
      Atherosclerosis, 2007;199(1):215-21.
      Species: Human
      Sample Type: Plasma
    52. Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
      Authors: Lin YL, Liang YC, Chiang BL
      J. Leukoc. Biol., 2007;82(6):1473-80.
      Species: Human
      Sample Type: Cell Culture Supernates
    53. Short communication: decreasing soluble CD30 and increasing IFN-gamma plasma levels are indicators of effective highly active antiretroviral therapy.
      Authors: Sadeghi M, Susal C, Daniel V, Naujokat C, Zimmermann R, Huth-Kuhne A, Opelz G
      AIDS Res. Hum. Retroviruses, 2007;23(7):886-90.
      Species: Human
      Sample Type: Plasma
    54. Expression of human GITRL on myeloid dendritic cells enhances their immunostimulatory function but does not abrogate the suppressive effect of CD4+CD25+ regulatory T cells.
      Authors: Tuyaerts S, Van Meirvenne S, Bonehill A, Heirman C, Corthals J, Waldmann H, Breckpot K, Thielemans K, Aerts JL
      J. Leukoc. Biol., 2007;82(1):93-105.
      Species: Human
      Sample Type: Cell Culture Supernates
    55. 1, 25 Dihydroxyvitamin D3 modulated cytokine response in pulmonary tuberculosis.
      Authors: Vidyarani M, Selvaraj P, Jawahar MS, Narayanan PR
      Cytokine, 2007;40(2):128-34.
      Species: Human
      Sample Type: Cell Culture Supernates
    56. An investigation of auto-reactivity after head injury.
      Authors: Cox AL, Coles AJ, Nortje J, Bradley PG, Chatfield DA, Thompson SJ, Menon DK
      J. Neuroimmunol., 2006;174(1):180-6.
      Species: Human
      Sample Type: Cell Culture Supernates
    57. Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction.
      Authors: Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S
      Gut, 2006;56(2):243-52.
      Species: Human
      Sample Type: Cell Culture Supernates
    58. Influence of eotaxin 67G>A polymorphism on plasma eotaxin concentrations in myocardial infarction survivors and healthy controls.
      Authors: Sheikine Y, Olsen B, Gharizadeh B, Jatta K, Tornvall P, Ghaderi M
      Atherosclerosis, 2006;189(2):458-63.
      Species: Human
      Sample Type: Serum
    59. Interleukin-6 and vascular endothelial growth factor release by renal cell carcinoma cells impedes lymphocyte-dendritic cell cross-talk.
      Authors: Cabillic F, Bouet-Toussaint F, Toutirais O, Rioux-Leclercq N, Fergelot P, de La Pintiere CT, Genetet N, Patard JJ, Catros-Quemener V
      Clin. Exp. Immunol., 2006;146(3):518-23.
      Species: Human
      Sample Type: Cell Culture Supernates
    60. Decreased plasma CXCL16/SR-PSOX concentration is associated with coronary artery disease.
      Authors: Sheikine Y, Bang CS, Nilsson L, Samnegard A, Hamsten A, Jonasson L, Eriksson P, Sirsjo A
      Atherosclerosis, 2006;188(2):462-6.
      Species: Human
      Sample Type: Serum
    61. Bordetella pertussis inhibition of interleukin-12 (IL-12) p70 in human monocyte-derived dendritic cells blocks IL-12 p35 through adenylate cyclase toxin-dependent cyclic AMP induction.
      Authors: Spensieri F, Fedele G, Fazio C, Nasso M, Stefanelli P, Mastrantonio P, Ausiello CM
      Infect. Immun., 2006;74(5):2831-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    62. Airway inflammation in iron ore miners exposed to dust and diesel exhaust.
      Authors: Adelroth E, Hedlund U, Blomberg A, Helleday R, Ledin MC, Levin JO, Pourazar J, Sandstrom T, Jarvholm B
      Eur. Respir. J., 2006;27(4):714-9.
      Species: Human
      Sample Type: Sputum
    63. Foxp3+CD4+CD25+ T cells control virus-specific memory T cells in chimpanzees that recovered from hepatitis C.
      Authors: Manigold T, Shin EC, Mizukoshi E, Mihalik K, Murthy KK, Rice CM, Piccirillo CA, Rehermann B
      Blood, 2006;107(11):4424-32.
      Species: Primate - Pan troglodytes (Chimpanzee)
      Sample Type: Cell Culture Supernates
    64. Celecoxib up-regulates the expression of the zeta chain of T cell receptor complex in tumor-infiltrating lymphocytes in human cervical cancer.
      Authors: Ferrandina G, Ranelletti FO, Legge F, Salutari V, Martinelli E, Fattorossi A, Lorusso D, Zannoni G, Vellone V, Paglia A, Scambia G
      Clin. Cancer Res., 2006;12(7):2055-60.
      Species: Human
      Sample Type: Plasma
    65. Expression of IL-10 in patients with ovarian carcinoma.
      Authors: Mustea A, Konsgen D, Braicu EI, Pirvulescu C, Sun P, Sofroni D, Lichtenegger W, Sehouli J
      Anticancer Res., 2006;26(2):1715-8.
      Species: Human
      Sample Type: Serum
    66. The potential of adiponectin in driving arthritis.
      Authors: Ehling A, Schaffler A, Herfarth H, Tarner IH, Anders S, Distler O, Paul G, Distler J, Gay S, Scholmerich J, Neumann E, Muller-Ladner U
      J. Immunol., 2006;176(7):4468-78.
      Species: Human
      Sample Type: Cell Culture Supernates
    67. No difference in the effects of clopidogrel and aspirin on inflammatory markers in patients with coronary heart disease.
      Authors: Solheim S, Pettersen AA, Arnesen H, Seljeflot I
      Thromb. Haemost., 2006;96(5):660-4.
      Species: Human
      Sample Type: Serum
    68. Induction of dendritic cell maturation by pertussis toxin and its B subunit differentially initiate Toll-like receptor 4-dependent signal transduction pathways.
      Authors: Wang ZY, Yang D, Chen Q, Leifer CA, Segal DM, Su SB, Caspi RR, Howard ZO, Oppenheim JJ
      Exp. Hematol., 2006;34(8):1115-24.
      Species: Human
      Sample Type: Cell Culture Supernates
    69. The apoptotic-cell receptor CR3, but not alphavbeta5, is a regulator of human dendritic-cell immunostimulatory function.
      Authors: Skoberne M, Somersan S, Almodovar W, Truong T, Petrova K, Henson PM, Bhardwaj N
      Blood, 2006;108(3):947-55.
      Species: Human
      Sample Type: Cell Culture Supernates
    70. Detection of interleukin 10 in cerebrospinal fluid of patients with subacute sclerosing panencephalitis.
      Authors: Mistchenko AS, Fornari MC, Viegas M, Barrero PR, Diez RA
      J. Neurovirol., 2005;11(1):66-9.
      Species: Human
      Sample Type: CSF
    71. Experimental heatstroke in baboon: analysis of the systemic inflammatory response.
      Authors: Bouchama A, Ollivier V, Roberts G, Al Mohanna F, de Prost D, Eldali A, Saussereau E, El-Sayed R, Chollet-Martin S
      Shock, 2005;24(4):332-5.
      Species: Primate - Papio hamadryas (Hamadyras Baboon)
      Sample Type: Plasma
    72. Relationship between atopic status and nasal interleukin 10 and 11 levels in infants with respiratory syncytial virus bronchiolitis.
      Authors: Chung HL, Kim WT, Kim JK, Choi EJ, Lee JH, Lee GH, Kim SG
      Ann. Allergy Asthma Immunol., 2005;94(2):267-72.
      Species: Human
      Sample Type: Nasal Secretion
    73. Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
      Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
      Microbes Infect., 2005;7(3):427-36.
      Species: Human
      Sample Type: Serum
    74. Effects of the active metabolite of leflunomide, A77 1726, on cytokine release and the MAPK signalling pathway in human rheumatoid arthritis synoviocytes.
      Authors: Vergne-Salle&lt;/LastName&gt;&lt;F P&lt;/Initial, Vergne-Salle P, Leger DY, Bertin P, Treves R, Beneytout JL, Liagre B
      Cytokine, 2005;31(5):335-48.
      Species: Human
      Sample Type: Cell Culture Supernates
    75. Inflammatory cytokine concentrations are elevated in seminal plasma of men with spinal cord injuries.
      Authors: Basu S, Aballa TC, Ferrell SM, Lynne CM, Brackett NL
      J. Androl., 2004;25(2):250-4.
      Species: Human
      Sample Type: Seminal Plasma
    76. Hepatocyte growth factor in polymorphonuclear leukocytes is increased in patients with systemic inflammatory response syndrome.
      Authors: Matsushima A, Ogura H, Koh T, Fujita K, Yoshiya K, Sumi Y, Hosotsubo H, Kuwagata Y, Tanaka H, Shimazu T, Sugimoto H
      J Trauma, 2004;56(2):259-64.
      Species: Human
      Sample Type: Plasma
    77. Effects of antiasthmatic agents on the functions of peripheral blood monocyte-derived dendritic cells from atopic patients.
      Authors: Saeki S, Matsuse H, Kondo Y, Machida I, Kawano T, Tomari S, Obase Y, Fukushima C, Kohno S
      J. Allergy Clin. Immunol., 2004;114(3):538-44.
      Species: Human
      Sample Type: Cell Culture Supernates
    78. Antifungal immune reactivity in nasal polyposis.
      Authors: Pitzurra L, Bellocchio S, Nocentini A, Bonifazi P, Scardazza R, Gallucci L, Stracci F, Simoncelli C, Bistoni F, Romani L
      Infect. Immun., 2004;72(12):7275-81.
      Species: Human
      Sample Type: Nasal Lavage
    79. Lack of phenotypic and functional impairment in dendritic cells from chimpanzees chronically infected with hepatitis C virus.
      Authors: Larsson M, Babcock E, Grakoui A, Shoukry N, Lauer G, Rice C, Walker C, Bhardwaj N
      J. Virol., 2004;78(12):6151-61.
      Species: Primate - Pan troglodytes (Chimpanzee)
      Sample Type: Cell Culture Supernates
    80. Changes in CSF S100B and cytokine concentrations in early-phase severe traumatic brain injury.
      Authors: Hayakata T, Shiozaki T, Tasaki O, Ikegawa H, Inoue Y, Toshiyuki F, Hosotubo H, Kieko F, Yamashita T, Tanaka H, Shimazu T, Sugimoto H
      Shock, 2004;22(2):102-7.
      Species: Human
      Sample Type: Serum
    81. High concentrations of circulating macrophage migration inhibitory factor in patients with severe blunt trauma: Is serum macrophage migration inhibitory factor concentration a valuable prognostic factor?
      Authors: Chuang CC, Hung CJ, Tsai MC, Chuang YC
      Crit. Care Med., 2004;32(3):734-9.
      Species: Human
      Sample Type: Serum
    82. Human plasmacytoid dendritic cells activated by CpG oligodeoxynucleotides induce the generation of CD4+CD25+ regulatory T cells.
      Authors: Moseman EA, Liang X, Dawson AJ, Panoskaltsis-Mortari A, Krieg AM, Liu YJ, Blazar BR, Chen W
      J. Immunol., 2004;173(7):4433-42.
      Species: Human
      Sample Type: Cell Culture Supernates
    83. Urinary IL-2 assay for monitoring intravesical bacillus Calmette-Guerin response of superficial bladder cancer during induction course and maintenance therapy.
      Authors: Saint F, Kurth N, Maille P, Vordos D, Hoznek A, Soyeux P, Patard JJ, Abbou CC, Chopin DK
      Int. J. Cancer, 2003;107(3):434-40.
      Species: Human
      Sample Type: Urine
    84. TGF-beta in human milk is associated with wheeze in infancy.
      Authors: Oddy WH, Halonen M, Martinez FD, Lohman IC, Stern DA, Kurzius-Spencer M, Guerra S, Wright AL
      J. Allergy Clin. Immunol., 2003;112(4):723-8.
      Species: Human
      Sample Type: Milk
    85. Cytokine pattern in blister fluid and sera of patients with pemphigus.
      Authors: Baroni A, Perfetto B, Ruocco E, Greco R, Criscuolo D, Ruocco V
      Dermatology (Basel), 2002;205(2):116-21.
      Species: Human
      Sample Type: Serum
    86. Endogenously expressed nef uncouples cytokine and chemokine production from membrane phenotypic maturation in dendritic cells.
      Authors: Messmer D, Jacque JM, Santisteban C, Bristow C, Han SY, Villamide-Herrera L, Mehlhop E, Marx PA, Steinman RM, Gettie A, Pope M
      J. Immunol., 2002;169(8):4172-82.
      Species: Human
      Sample Type: Cell Culture Supernates
    87. Intraperitoneal bispecific antibody (HEA125xOKT3) therapy inhibits malignant ascites production in advanced ovarian carcinoma.
      Authors: Marme A, Strauss G, Bastert G, Grischke EM, Moldenhauer G
      Int. J. Cancer, 2002;101(2):183-9.
      Species: Human
      Sample Type: Ascites Fluid
    88. Chimeric co-stimulatory molecules that selectively act through CD28 or CTLA-4 on human T cells.
      Authors: Lazetic S, Leong SR, Chang JC, Ong R, Dawes G, Punnonen J
      J. Biol. Chem., 2002;277(41):38660-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    89. Cytokine profile in Behcet's disease patients. Relationship with disease activity.
      Authors: Hamzaoui K, Hamzaoui A, Guemira F, Bessioud M, Hamza M, Ayed K
      Scand. J. Rheumatol., 2002;31(4):205-10.
      Species: Human
      Sample Type: Serum
    90. HIV-1 protein Vpr suppresses IL-12 production from human monocytes by enhancing glucocorticoid action: potential implications of Vpr coactivator activity for the innate and cellular immunity deficits observed in HIV-1 infection.
      Authors: Mirani M, Elenkov I, Volpi S, Hiroi N, Chrousos GP, Kino T
      J. Immunol., 2002;169(11):6361-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    91. Skewing of cytotoxic activity and chemokine production, but not of chemokine receptor expression, in human type-1/-2 gamma delta T lymphocytes.
      Authors: D&amp;amp;apos;Ambrosio D
      Eur. J. Immunol., 2002;32(10):2934-43.
      Species: Human
      Sample Type: Cell Culture Supernates
    92. Interleukin-11 induces Th2 polarization of human CD4(+) T cells.
      Authors: Curti A, Ratta M, Corinti S, Girolomoni G, Ricci F, Tazzari P, Siena M, Grande A, Fogli M, Tura S, Lemoli RM
      Blood, 2001;97(9):2758-63.
      Species: Human
      Sample Type: Cell Culture Supernates
    93. Structural features of peptide analogs of human histocompatibility leukocyte antigen class I epitopes that are more potent and immunogenic than wild-type peptide.
      Authors: Tangri S, Ishioka GY, Huang X, Sidney J, Southwood S, Fikes J, Sette A
      J. Exp. Med., 2001;194(6):833-46.
      Species: Human
      Sample Type: Cell Culture Supernates
    94. Mature dendritic cells infiltrate the T cell-rich region of oral mucosa in chronic periodontitis: in situ, in vivo, and in vitro studies.
      Authors: Jotwani R, Palucka AK, Al-Quotub M, Nouri-Shirazi M, Kim J, Bell D, Banchereau J, Cutler CW
      J. Immunol., 2001;167(8):4693-700.
      Species: Human
      Sample Type: Gingival Crevicular Fluid (GCF)
    95. Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
      Authors: Kadowaki N, Antonenko S, Lau JY, Liu YJ
      J. Exp. Med., 2000;192(2):219-26.
      Species: Human
      Sample Type: Cell Culture Supernates
    96. Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGF-beta, PGE2, and PAF.
      Authors: Fadok, V A, Bratton, D L, Konowal, A, Freed, P W, Westcott, J Y, Henson, P M
      J Clin Invest, 1998;101(4):890-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    97. Ex vivo adenoviral vector gene delivery results in decreased vector-associated inflammation pre- and post-lung transplantation in the pig.
      Authors: Yeung JC, Wagnetz D, Cypel M, Rubacha M, Koike T, Chun YM, Hu J, Waddell TK, Hwang DM, Liu M, Keshavjee S
      Mol. Ther., 212;20(6):1204-11.
      Species: Complex Species Category
      Sample Type: Plasma
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     Human IL-10 Quantikine ELISA Kit D1000B
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     Human IL-10 Quantikine ELISA Kit D1000B
    : Human IL-10 Quantikine ELISA Kit [D1000B]

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    Other Experimental DetailsUsed human synovial fluid samples with a dilution of 1 in 5 using DI water. Worked beautifully!
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     Human IL-10 Quantikine ELISA Kit D1000B
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    Other Experimental DetailsThe Human IL-10 Quantikine ELISA kit is used for Diagnostic use.
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     Human IL-10 Quantikine ELISA Kit D1000B
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