Human IL-10 Quantikine HS ELISA Kit

(33 citations)   
  • Assay Length
    5.5 hours
  • Sample Type & Volume Required Per Well
    Serum (200 uL), EDTA Plasma (200 uL), Citrate Plasma (200 uL)
  • Sensitivity
    0.17 pg/mL
  • Assay Range
    0.8 - 50 pg/mL (Serum, EDTA Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human IL-10
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC41, Quantikine Immunoassay Control Group 10 - Please Inquire
Product Summary
The Quantikine HS Human IL-10 Immunoassay is a 5.5 hour solid phase ELISA designed to measure IL-10 in serum and plasma. It contains Sf 21-expressed recombinant human IL-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-10. Results obtained using natural human IL-10 showed linear curves that are parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-10.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020353535
Mean2.3610.7211.6810.420.9
Standard Deviation0.220.620.970.220.811.78
CV%9.45.84.612.87.88.5

Recovery

The recovery of IL-10 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Citrate Plasma (n=4) 93 81-106
EDTA Plasma (n=4) 90 84-99
Serum (n=4) 100 94-108
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of IL-10 in various matrices and diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-10 Quantikine HS ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-10
Interleukin-10 functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.
    • Long Name
      Interleukin 10
    • Entrez Gene IDs
      3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral);
    • Alternate Names
      CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF;
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    33 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. Serum and peritoneal fluid concentrations of soluble human leukocyte antigen, tumor necrosis factor alpha and interleukin 10 in patients with selected ovarian pathologies
      Authors: O Sipak-Szmi, P W?odarski, E Ronin-Walk, A Niedzielsk, B Karakiewic, S S?uczanows, M Laszczy?sk, W Malinowski
      J Ovarian Res, 2017;10(1):25.
      Species: Human
      Sample Type: Serum
    2. Annexin A1 in blood mononuclear cells from patients with coronary artery disease: Its association with inflammatory status and glucocorticoid sensitivity
      Authors: I Bergström, AK Lundberg, S Jönsson, E Särndahl, J Ernerudh, L Jonasson
      PLoS ONE, 2017;12(3):e0174177.
      Species: Human
      Sample Type: Plasma
    3. Aberrant expression of interleukin-10 in rheumatoid arthritis: Relationship with IL10 haplotypes and autoantibodies
      Authors: J Hernández-, E Oregón-Rom, M Vázquez-Vi, S García-Are, Y Valle, JR Padilla-Gu, IV Román-Fern, CA Palafox-Sá, GE Martínez-B, JF Muñoz-Vall
      Cytokine, 2017;95(0):88-96.
      Species: Human
      Sample Type: Serum
    4. Acute phase IL-10 plasma concentration associates with the high risk sources of cardiogenic stroke.
      Authors: Arponen O, Muuronen A, Taina M, Sipola P, Hedman M, Jakala P, Vanninen R, Pulkki K, Mustonen P
      PLoS ONE, 2015;10(4):e0120910.
      Species: Human
      Sample Type: Plasma
    5. Mucosal effects of tenofovir 1% gel.
      Authors: Hladik F, Burgener A, Ballweber L, Gottardo R, Vojtech L, Fourati S, Dai J, Cameron M, Strobl J, Hughes S, Hoesley C, Andrew P, Johnson S, Piper J, Friend D, Ball T, Cranston R, Mayer K, McElrath M, McGowan I
      Elife, 2015;4(0):.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. Genome-Wide Association Study Identifies That the ABO Blood Group System Influences Interleukin-10 Levels and the Risk of Clinical Events in Patients with Acute Coronary Syndrome.
      Authors: Johansson A, Alfredsson J, Eriksson N, Wallentin L, Siegbahn A
      PLoS ONE, 2015;10(11):e0142518.
      Species: Human
      Sample Type: Plasma
    7. Psychological stress exerts effects on pathogenesis of hepatitis B via type-1/type-2 cytokines shift toward type-2 cytokine response.
      Authors: He Y, Gao H, Li X, Zhao Y
      PLoS ONE, 2014;9(8):e105530.
      Species: Human
      Sample Type: Plasma
    8. The impact of two different transfusion strategies on patient immune response during major abdominal surgery: a preliminary report.
      Authors: Theodoraki K, Markatou M, Rizos D, Fassoulaki A
      J Immunol Res, 2014;2014(0):945829.
      Species: Human
      Sample Type: Plasma
    9. Whole-Body UVB Irradiation during Allogeneic Hematopoietic Cell Transplantation Is Safe and Decreases Acute Graft-versus-Host Disease.
      Authors: Kreutz M, Karrer S, Hoffmann P
      J. Invest. Dermatol., 2012;132(1):179-87.
      Species: Human
      Sample Type: Serum
    10. Time-course of changes in inflammatory response after whole-body cryotherapy multi exposures following severe exercise.
      Authors: Pournot H, Bieuzen F, Louis J, Fillard JR, Barbiche E, Hausswirth C
      PLoS ONE, 2011;6(7):e22748.
      Species: Human
      Sample Type: Plasma
    11. Inflammation associated with obesity: relationship with blood and bone marrow endothelial cells.
      Authors: McGuire TR, Brusnahan SK, Bilek LD, Jackson JD, Kessinger MA, Berger AM, Garvin KL, O'Kane BJ, Tuljapurkar SR, Sharp JG
      Obesity (Silver Spring), 2011;19(11):2130-6.
      Species: Human
      Sample Type: Plasma
    12. SIRT1 mRNA expression may be associated with energy expenditure and insulin sensitivity.
      Authors: Rutanen J, Yaluri N, Modi S, Pihlajamaki J, Vanttinen M, Itkonen P, Kainulainen S, Yamamoto H, Lagouge M, Sinclair DA, Elliott P, Westphal C, Auwerx J, Laakso M
      Diabetes, 2010;59(4):829-35.
      Species: Human
      Sample Type: Plasma
    13. Changes in cytokine levels during acute hyperinsulinemia in offspring of type 2 diabetic subjects.
      Authors: Ruotsalainen E, Stancakova A, Vauhkonen I, Salmenniemi U, Pihlajamaki J, Punnonen K, Laakso M
      Atherosclerosis, 2010;210(2):536-41.
      Species: Human
      Sample Type: Plasma
    14. Interleukin-18 is a strong predictor of cardiovascular events in elderly men with the metabolic syndrome: synergistic effect of inflammation and hyperglycemia.
      Authors: Troseid M, Seljeflot I, Hjerkinn EM, Arnesen H
      Diabetes Care, 2009;32(3):486-92.
      Species: Human
      Sample Type: Serum
    15. Age-dependent mobilization of circulating endothelial progenitor cells in infants and young children undergoing cardiac surgery with cardiopulmonary bypass.
      Authors: Sun Y, Yi D, Wang Y, Zheng R, Sun G, Wang J, Liu Y, Ren J, Wang Y, Zhang S, Gu C, Pei J
      Cytokine, 2009;47(3):206-13.
      Species: Human
      Sample Type: Plasma
    16. Two large preoperative doses of erythropoietin do not reduce the systemic inflammatory response to cardiac surgery.
      Authors: Poulsen TD, Andersen LW, Steinbruchel D, Gotze JP, Jorgensen OS, Olsen NV
      J. Cardiothorac. Vasc. Anesth., 2009;23(3):316-23.
      Species: Human
      Sample Type: Plasma
    17. Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
      Authors: Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G
      Transplantation, 2009;88(9):1109-16.
      Species: Human
      Sample Type: Urine
    18. In vivo transmigrated monocytes from patients with stable coronary artery disease have a reduced expression of CD11b.
      Authors: Paulsson JM, Dadfar E, Held C, Jacobson SH, Lundahl J
      Clin. Exp. Immunol., 2008;153(2):196-204.
      Species: Human
      Sample Type: Serum
    19. Duffy antigen modifies the chemokine response in human endotoxemia.
      Authors: Mayr FB, Spiel AO, Leitner JM, Firbas C, Kliegel T, Jilma-Stohlawetz P, Derendorf H, Jilma B
      Crit. Care Med., 2008;36(1):159-65.
      Species: Human
      Sample Type: Plasma
    20. Angiopoietin-2, marker and mediator of endothelial activation with prognostic significance early after trauma?
      Authors: Ganter MT, Cohen MJ, Brohi K, Chesebro BB, Staudenmayer KL, Rahn P, Christiaans SC, Bir ND, Pittet JF
      Ann. Surg., 2008;247(2):320-6.
      Species: Human
      Sample Type: Plasma
    21. Co-occurrence of IgA antibodies against ethanol metabolites and tissue transglutaminase in alcohol consumers: correlation with proinflammatory cytokines and markers of fibrogenesis.
      Authors: Koivisto H, Hietala J, Anttila P, Niemela O
      Dig. Dis. Sci., 2007;53(2):500-5.
      Species: Human
      Sample Type: Serum
    22. Recombinant human antithrombin inhibits thrombin formation and interleukin 6 release in human endotoxemia.
      Authors: Leitner JM, Firbas C, Mayr FB, Reiter RA, Steinlechner B, Jilma B
      Clin. Pharmacol. Ther., 2006;79(1):23-34.
      Species: Human
      Sample Type: Plasma
    23. Dysregulated monocyte iron homeostasis and erythropoietin formation in patients with anemia of chronic disease.
      Authors: Theurl I, Mattle V, Seifert M, Mariani M, Marth C, Weiss G
      Blood, 2006;107(10):4142-8.
      Species: Human
      Sample Type: Serum
    24. Anti-inflammatory cytokines in gingival crevicular fluid in patients with periodontitis and rheumatoid arthritis: a preliminary report.
      Authors: Bozkurt FY, Yetkin Ay Z, Berker E, Tepe E, Akkus S
      Cytokine, 2006;35(3):180-5.
      Species: Human
      Sample Type: Gingival Crevicular Fluid (GCF)
    25. The activity of N-acetyl-beta-D-glucosaminidase and tumor necrosis factor-alpha at early stage of diabetic retinopathy development in type 1 diabetes mellitus children.
      Authors: Mysliwiec M, Zorena K, Balcerska A, Mysliwska J, Lipowski P, Raczynska K
      Clin. Biochem., 2006;39(8):851-6.
      Species: Human
      Sample Type: Serum
    26. Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
      Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
      Microbes Infect., 2005;7(3):427-36.
      Species: Human
      Sample Type: Serum
    27. Strong inflammatory cytokine response in male and strong anti-inflammatory response in female kidney transplant recipients with urinary tract infection.
      Authors: Sadeghi M, Daniel V, Naujokat C, Wiesel M, Hergesell O, Opelz G
      Transpl. Int., 2005;18(2):177-85.
      Species: Human
      Sample Type: Plasma
    28. Interleukin-8, monocyte chemoattractant protein-1 and IL-10 in the vitreous fluid of patients with proliferative diabetic retinopathy.
      Authors: Hernandez C, Segura RM, Fonollosa A, Carrasco E, Francisco G, Simo R
      Diabet. Med., 2005;22(6):719-22.
      Species: Human
      Sample Type: Vitreous Humor
    29. Cytokines and adhesion molecules in multiple sclerosis patients treated with interferon-beta1b.
      Authors: Jensen J, Krakauer M, Sellebjerg F
      Cytokine, 2005;29(1):24-30.
      Species: Human
      Sample Type: Plasma
    30. Immunodetection of a hepatitis C virus (HCV) antigen and Thl/Th2 cytokines in cerebrospinal fluid of meningitis patients.
      Authors: Attallah AM, Ibrahim GG
      J Immunoassay Immunochem, 2004;25(4):313-20.
      Species: Human
      Sample Type: Serum
    31. Escape from cardiomyocyte apoptosis by enterovirus persistence due to elevated soluble Fas-receptors.
      Authors: Alter P, Maisch B
      Z Kardiol, 2004;93(7):524-32.
      Species: Human
      Sample Type: Serum
    32. Comparative effects of AT1-antagonism and angiotensin-converting enzyme inhibition on markers of inflammation and platelet aggregation in patients with coronary artery disease.
      Authors: Schieffer B, Bunte C, Witte J, Hoeper K, Boger RH, Schwedhelm E, Drexler H
      J. Am. Coll. Cardiol., 2004;44(2):362-8.
      Species: Human
      Sample Type: Serum
    33. Low levels of interferon-gamma in nasal fluid accompany raised levels of T-helper 2 cytokines in children with ongoing allergic rhinitis.
      Authors: Benson M, Strannegard IL, Wennergren G, Strannegard O
      Pediatr Allergy Immunol, 2000;11(1):20-8.
      Species: Human
      Sample Type: Nasal Lavage
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