• Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
  • Sensitivity
    57.4 pg/mL
  • Assay Range
    125.0 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human IL-13
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC20, Quantikine Immunoassay Control Group 3 - Please Inquire
Product Summary
The Quantikine Human IL-13 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-13 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-13 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-13 accurately. Results obtained using natural human IL-13 showed dose response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-13.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample112233112233
n202020202020202020202020
Mean36028780010312002160231929781893218951662
Standard Deviation12.91839.327.543.263.122.233.679.352.6108162
CV%3.66.34.92.72.23.9711.39.75.65.79.7

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean352960191936510872199
Standard Deviation11.121.333.325.863.1105
CV%3.22.21.77.15.84.8

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean33587817373278981906
Standard Deviation215440.920.634124
CV%6.36.22.46.33.86.5

Recovery

The recovery of IL-13 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 108 100-120
EDTA Plasma (n=4) 106 98-120
Heparin Plasma (n=4) 104 94-120
Serum (n=4) 102 95-113
Linearity
To assess linearity of the assay, samples were spiked with high concentrations of IL-13 and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-13 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-13
IL-13 (Interleukin-13) plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. It promotes B cell activation, immunoglobulin class switching to IgE, and the upregulation of CD23/Fc epsilon RII. IL-13 signals through a receptor complex containing IL-13 R alpha 1 and IL-4 R alpha. This complex also functions as the type 2 IL-4 receptor. IL-13 R alpha 2 binds IL-13 and prevents IL-13 from signaling through the IL-13 R alpha 1/IL-4 R alpha complex. IL-13-bound IL-13 R alpha 2 can directly promote tumor cell invasiveness and the development of tissue fibrosis.
    • Long Name
      Interleukin 13
    • Entrez Gene IDs
      3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate);
    • Alternate Names
      ALRHMGC116789; BHR1interleukin-13; IL13; IL-13; IL-13MGC116788; interleukin 13; MGC116786; NC30; P600;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    19 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. IL-33 reflects dynamics of disease activity in patients with autoimmune hemolytic anemia by regulating autoantibody production.
      Authors: Bu X, Zhang T, Wang C, Ren T, Wen Z
      J Transl Med, 2015;13(0):381.
      Species: Human
      Sample Type: Cell Culture Supernates
    2. Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis.
      Authors: Takei S, Hoshino T, Matsunaga K, Sakazaki Y, Sawada M, Oda H, Takenaka S, Imaoka H, Kinoshita T, Honda S, Ida H, Fukuda TA, Aizawa H
      Arthritis Res. Ther., 2011;13(2):R52.
      Species: Human
      Sample Type: Serum
    3. Thymic stromal lymphopoietin-induced human asthmatic airway epithelial cell proliferation through an IL-13-dependent pathway.
      Authors: Semlali A, Jacques E, Koussih L, Gounni AS, Chakir J
      J. Allergy Clin. Immunol., 2010;125(4):844-50.
      Species: Human
      Sample Type: Cell Culture Supernates
    4. Mast cell-dependent contraction of human airway smooth muscle cell-containing collagen gels: influence of cytokines, matrix metalloproteases, and serine proteases.
      Authors: Margulis A, Nocka KH, Brennan AM, Deng B, Fleming M, Goldman SJ, Kasaian MT
      J. Immunol., 2009;183(3):1739-50.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
      Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
      J. Allergy Clin. Immunol., 2009;123(1):59-66.e4.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. Detection of pancreatic cancer using antibody microarray-based serum protein profiling.
      Authors: Ingvarsson J, Wingren C, Carlsson A, Ellmark P, Wahren B, Engstrom G, Harmenberg U, Krogh M, Peterson C, Borrebaeck CA
      Proteomics, 2008;8(11):2211-9.
      Species: Human
      Sample Type: Serum
    7. Development of an in vitro potency bioassay for therapeutic IL-13 antagonists: the A-549 cell bioassay.
      Authors: Miller R, Sadhukhan R, Wu C
      J. Immunol. Methods, 2008;334(1):134-41.
      Species: Human
      Sample Type: Cell Culture Supernates
    8. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
      Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
      Eur. J. Immunol., 2008;38(4):1012-23.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. Mycobacterium bovis Bacillus Calmette-Guerin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells.
      Authors: Yokoi T, Amakawa R, Tanijiri T, Sugimoto H, Torii Y, Amuro H, Son Y, Tajima K, Liu YJ, Ito T, Fukuhara S
      Int. Immunol., 2008;20(10):1321-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. IL-13 involvement in eosinophilic esophagitis: transcriptome analysis and reversibility with glucocorticoids.
      Authors: Blanchard C, Mingler MK, Vicario M, Abonia JP, Wu YY, Lu TX, Collins MH, Putnam PE, Wells SI, Rothenberg ME
      J. Allergy Clin. Immunol., 2007;120(6):1292-300.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
      Authors: Lin YL, Liang YC, Chiang BL
      J. Leukoc. Biol., 2007;82(6):1473-80.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Expression of interleukin-18 and caspase-1 in cutaneous T-cell lymphoma.
      Authors: Yamanaka K, Clark R, Dowgiert R, Hurwitz D, Shibata M, Rich BE, Hirahara K, Jones DA, Eapen S, Mizutani H, Kupper TS
      Clin. Cancer Res., 2006;12(2):376-82.
      Species: Human
      Sample Type: Plasma
    13. A network-based analysis of the late-phase reaction of the skin.
      Authors: Benson M, Langston MA, Adner M, Andersson B, Torinssson-Naluai A, Cardell LO
      J. Allergy Clin. Immunol., 2006;118(1):220-5.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. Chemokines indicate allergic bronchopulmonary aspergillosis in patients with cystic fibrosis.
      Authors: Hartl D, Latzin P, Zissel G, Krane M, Krauss-Etschmann S, Griese M
      Am. J. Respir. Crit. Care Med., 2006;173(12):1370-6.
      Species: Human
      Sample Type: Serum
    15. Relationship between cytokines and the embryotoxicity of hydrosalpingeal fluid.
      Authors: Bedaiwy MA, Falcone T, Goldberg JM, Attaran M, Sharma R, Miller K, Nelson DR, Agarwal A
      J. Assist. Reprod. Genet., 2005;22(4):161-5.
      Species: Human
      Sample Type: Hypersalpingeal Fluid (HSF)
    16. Differential expression of interleukins IL-13 and IL-15 in normal ovarian tissue and ovarian carcinomas.
      Authors: Ripley D, Shoup B, Majewski A
      Gynecol. Oncol., 2004;92(3):761-8.
      Species: Human
      Sample Type: Tissue Homogenates
    17. Differential expression of interleukins IL-13 and IL-15 in normal ovarian tissue and ovarian carcinomas.
      Authors: Ripley D, Shoup B, Majewski A
      Gynecol. Oncol., 2004;92(3):761-8.
      Species: Human
      Sample Type: Tissue Homogenates
    18. Implications of serum TNF-beta and IL-13 in the treatment response of childhood nephrotic syndrome.
      Authors: Tain YL, Chen TY, Yang KD
      Cytokine, 2003;21(3):155-9.
      Species: Human
      Sample Type: Serum
    19. Modulation of human immune response by Echinococcus granulosus antigen B and its possible role in evading host defenses.
      Authors: Rigano R, Profumo E, Bruschi F, Carulli G, Azzara A, Ioppolo S, Buttari B, Ortona E, Margutti P, Teggi A, Siracusano A
      Infect. Immun., 2001;69(1):288-96.
      Species: Human
      Sample Type: Cell Culture Supernates
    Expand to show all 19 Citations
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    Sample TestedA375 human melanoma cell line

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