Human IL-13 Quantikine ELISA Kit

R&D Systems | Catalog # D1300B

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)

Sensitivity

57.4 pg/mL

Assay Range

125-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Human IL-13 Quantikine ELISA Kit

The Quantikine Human IL-13 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-13 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-13 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-13 accurately. Results obtained using natural human IL-13 showed dose response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-13.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human IL-13

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 360 1031 2002 319 932 1895
Standard Deviation 12.9 27.5 43.2 22.2 52.6 108
CV% 3.6 2.7 2.2 7.0 5.6 5.7

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 352 960 1919 365 1087 2199
Standard Deviation 11.1 21.3 33.3 25.8 63.1 105
CV% 3.2 2.2 1.7 7.1 5.8 4.8

Recovery for Human IL-13 Quantikine ELISA Kit

The recovery of IL-13 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 108 100-120
EDTA Plasma (n=4) 106 98-120
Heparin Plasma (n=4) 104 94-120
Serum (n=4) 102 95-113

Linearity

To assess linearity of the assay, samples were spiked with high concentrations of IL-13 and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human IL-13 ELISA Linearity

Scientific Data Images for Human IL-13 Quantikine ELISA Kit

Human IL-13 ELISA Calibrator Diluent RD5R Standard Curve

Human IL-13 ELISA Calibrator Diluent RD5R Standard Curve

Human IL-13 ELISA Calibrator Diluent RD6-64 Standard Curve

Human IL-13 ELISA Calibrator Diluent RD6-64 Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-13

IL-13 (Interleukin-13) plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. It promotes B cell activation, immunoglobulin class switching to IgE, and the upregulation of CD23/Fc epsilon RII. IL-13 signals through a receptor complex containing IL-13 R alpha 1 and IL-4 R alpha. This complex also functions as the type 2 IL-4 receptor. IL-13 R alpha 2 binds IL-13 and prevents IL-13 from signaling through the IL-13 R alpha 1/IL-4 R alpha complex. IL-13-bound IL-13 R alpha 2 can directly promote tumor cell invasiveness and the development of tissue fibrosis.

Long Name

Interleukin 13

Alternate Names

IL13, NC30, P600

Entrez Gene IDs

3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)

Gene Symbol

IL13

Additional IL-13 Products

Product Documents for Human IL-13 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IL-13 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human IL-13 Quantikine ELISA Kit

Customer Reviews for Human IL-13 Quantikine ELISA Kit (4)

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Showing  1 - 4 of 4 reviews Showing All
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  • Human IL-13 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 10/29/2020
    Clear instructions, Good results
    Human IL-13 Quantikine ELISA Kit D1300B
  • Human IL-13 Quantikine ELISA Kit
    Name: Evan Williams
    Sample Tested: EDTA Plasma and Sputum
    Verified Customer | Posted 10/28/2020
    Kit was easy to use. Il-13 was detectable in human sputum, but not plasma.
    Human IL-13 Quantikine ELISA Kit D1300B
  • Human IL-13 Quantikine ELISA Kit
    Name: Gerre Lafond
    Sample Tested: CHO Chinese hamster ovary cell line
    Verified Customer | Posted 05/17/2018
  • Human IL-13 Quantikine ELISA Kit
    Name: Alisha Freeman
    Sample Tested: A375 human melanoma cell line
    Verified Customer | Posted 11/27/2017

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Protocols

View specific protocols for Human IL-13 Quantikine ELISA Kit (D1300B):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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