Human IL-13 R alpha 2 Antibody

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Detection of Human IL‑13 R alpha 2 by Western Blot.
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Product Details
Citations (8)
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Human IL-13 R alpha 2 Antibody Summary

Species Reactivity
Detects human IL-13 R alpha 2 in direct ELISAs and Western blots. In Western blots, this antibody shows approximately 5% cross‑reactivity with recombinant human (rh) IL‑4 R and rhIL‑9 R and no cross-reactivity with rhIL‑5 R alpha, rhIL‑5 R beta, rhIL‑13 R alpha 1, or recombinant mouse IL‑13 R alpha 2.
Monoclonal Mouse IgG1 Clone # 83834
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human IL‑13 R alpha 2
Accession # Q14627
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IL-13 Ra2 antibody by Western Blot. View Larger

Detection of Human IL‑13 R alpha 2 by Western Blot. Western blot shows lysates of human placenta tissue and human testis tissue. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human IL-13 Ra2 Monoclonal Antibody (Catalog # MAB614) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-13 Ra2 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-13 R alpha 2

Two type I membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with high affinity. The lower affinity IL-13 binding protein is now referred to as IL-13 R alpha 1 and is also known as CD213a. IL-13 R alpha 1 combines with IL-4 R alpha to form a high affinity receptor complex capable of transducing an IL-13-dependent proliferative signal. The higher affinity IL-13 binding protein, now referred to as IL-13 R alpha 2, does not induce a signal and acts as a decoy receptor.

Long Name
Interleukin 13 Receptor alpha 2
Entrez Gene IDs
3598 (Human); 16165 (Mouse)
Alternate Names
cancer/testis antigen 19; CD213a2 antigen; CD213a2; CT19; IL-13 R alpha 2; IL-13 receptor subunit alpha-2; IL13BP; IL13R alpha 2; IL-13R subunit alpha-2; IL13R; IL-13R; IL13RA2; IL-13Ra2; IL-13R-alpha-2; interleukin 13 binding protein; interleukin 13 receptor alpha 2 chain; interleukin 13 receptor, alpha 2; interleukin-13 receptor subunit alpha-2; Interleukin-13-binding protein

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Citations for Human IL-13 R alpha 2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Silencing IL-13R alpha 2 Promotes Glioblastoma Cell Death via Endogenous Signaling
    Authors: Linda C. Hsi, Suman Kundu, Juan Palomo, Bo Xu, Ryan Ficco, Michael A. Vogelbaum et al.
    Molecular Cancer Therapeutics
  2. Differential expression of the alpha2 chain of the interleukin-13 receptor in metastatic human prostate cancer ARCaPM cells
    Authors: Hui He, Jianchun Xu, Peter S. Nelson, Fray F. Marshall, Leland W. K. Chung, Haiyen E. Zhau et al.
    The Prostate
  3. Cigarette smoke extracts induce overexpression of the proto-oncogenic gene interleukin-13 receptor ?2 through activation of the PKA-CREB signaling pathway to trigger malignant transformation of lung vascular endothelial cells and angiogenesis
    Authors: Mei Meng
    Cell. Signal, 2016-12-13;0(0):.
  4. Role of IL13RA2 in Sunitinib Resistance in Clear Cell Renal Cell Carcinoma.
    Authors: Shibasaki N, Yamasaki T, Kanno T, Arakaki R, Sakamoto H, Utsunomiya N, Inoue T, Tsuruyama T, Nakamura E, Ogawa O, Kamba T
    PLoS ONE, 2015-06-26;10(6):e0130980.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Suppression of human glioma xenografts with second-generation IL13R-specific chimeric antigen receptor-modified T cells.
    Authors: Kong S, Sengupta S, Tyler B, Bais A, Ma Q, Doucette S, Zhou J, Sahin A, Carter B, Brem H, Junghans R, Sampath P
    Clin Cancer Res, 2012-09-10;18(21):5949-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Level of expression of IL-13R alpha 2 impacts receptor distribution and IL-13 signaling.
    Authors: Daines MO, Tabata Y, Walker BA, Chen W, Warrier MR, Basu S, Hershey GK
    J. Immunol., 2006-06-15;176(12):7495-501.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  7. Soluble interleukin-13Ralpha2 decoy receptor inhibits Hodgkin's lymphoma growth in vitro and in vivo.
    Authors: Trieu Y, Wen XY, Skinnider BF, Bray MR, Li Z, Claudio JO, Masih-Khan E, Zhu YX, Trudel S, McCart JA, Mak TW, Stewart AK
    Cancer Res., 2004-05-01;64(9):3271-5.
    Species: Human
    Sample Types: Cell Culture Supernates, Cell Lysates
    Applications: ELISA Development, Western Blot
  8. Identification of the alpha 2 chain of interleukin‐13 receptor as a potential biomarker for predicting castration resistance of prostate cancer using patient‐derived xenograft models
    Authors: Takahiro Nagai, Naoki Terada, Masato Fujii, Yasuhisa Nagata, Kozue Nakahara, Shoichiro Mukai et al.
    Cancer Rep (Hoboken)


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