Detects human IL-13 R alpha 2 in direct ELISAs and Western blots. In Western blots, this antibody shows approximately 5% cross‑reactivity with recombinant human (rh) IL‑4 R and rhIL‑9 R and no cross-reactivity with rhIL‑5 R alpha, rhIL‑5 R beta, rhIL‑13 R alpha 1, or recombinant mouse IL‑13 R alpha 2.
Monoclonal Mouse IgG1 Clone # 83834
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human IL‑13 R alpha 2 Cys22-Leu342 Accession # Q14627
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human IL‑13 R alpha 2 by Western Blot.
Western blot shows lysates of human placenta tissue and human testis tissue. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human IL‑13 R alpha 2 Monoclonal Antibody (Catalog # MAB614) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL‑13 R alpha 2 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-13 R alpha 2
Two type I membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with high affinity. The lower affinity IL-13 binding protein is now referred to as IL-13 R alpha 1 and is also known as CD213a. IL-13 R alpha 1 combines with IL-4 R alpha to form a high affinity receptor complex capable of transducing an IL-13-dependent proliferative signal. The higher affinity IL-13 binding protein, now referred to as IL-13 R alpha 2, does not induce a signal and acts as a decoy receptor.
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