Human IL-13 R alpha 2 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human IL-13 R alpha 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse IL-13 R alpha 2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL‑13 R alpha 2
    Accession # Q14627
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑13 R alpha 2 in A375 Human Cell Line by Flow Cytometry. A375 human melanoma cell line was stained with Goat Anti-Human IL‑13 R alpha 2 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB614A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-13 R alpha 2
Interleukin-13 Receptor alpha 2 (IL-13 R alpha 2), also CD213 alpha 2, is a 55-57 kDa member of the type I cytokine receptor family of molecules. Human IL-13 R alpha 2 is a 354 amino acid (aa) type I transmembrane glycoprotein that contains a 317 aa extracellular domain (ECD) characterized by three fibronectin type III domains (aa 27-343). It is expressed by a limited number of cell types, principally those associated with the respiratory system. These cells include fibroblasts, type II alveolar and visceral smooth muscle cells, pseudostratified ciliated epithelium, goblet cells and possibly monocytes. Notably, IL-13 R alpha 2 is often found to be intracellular, rather than plasma membrane expressed, and this may reflect storage deposits that can be quickly accessed when needed. IL-13 R alpha 2 is one of two IL-13Rs that bind IL-13. The principal, but low affinity receptor for IL-13 is a 65 kDa transmembrane protein termed IL-13 R alpha 1. Following binding to IL-13, this protein complexes with an adjacent IL-4 R alpha receptor on the cell surface to form a high affinity, functional receptor signaling complex for IL-13. Within this system, IL-13 R alpha 2 has been described as a "decoy receptor" that binds to circulating IL-13, rendering it unavailable for IL-13 R alpha 1 binding. Supporting this view is the fact that there does not appear to be any canonical signaling motif in IL-13 R alpha 2s short cytoplasmic tail, and the fact that soluble IL-13 R alpha 2 will bind to IL-13 and neutralize its effects on target cells in culture. The latter point is of particular importance when it is recognized that IL-13 R alpha 2 has a naturally circulating soluble splice form in mouse. Studies in human, however, suggest a more complicated situation. First, it is unclear if human actually has circulating IL-13 R alpha 2. Unlike in mouse, there is no potential alternative splice form in human that could generate a soluble isoform. And while MMP-8 has been identified as a potential mediator of IL-13 R alpha 2 proteolytic cleavage, such activity would appear to generate rather small quantities of soluble IL-13 R alpha 2. As a transmembrane protein, IL-13 R alpha 2 is suggested to interact with IL-4 R alpha, and this likely will result in a downregulation of IL-13 activity. Thus, IL-13 R alpha 2, while not a decoy receptor, could act as a negative modulator of IL-13 signaling. On the other hand, the cytoplasmic tail of IL-13 R alpha 2 is now believed to stimulate AP-1 activity, and the IL-13:IL-13 R alpha 2 transmembrane complex has been identified as a component of a larger signaling receptor complex for the GH-18 (Glycosyl Hydrolase 18) family member YKL40/Chi3L1. As such, IL-13 R alpha 2 is likely to exhibit multiple actions in a system and species-specific manner. Over aa 27-342, human and mouse IL-13 R alpha 2 share 64% aa sequence identity.
  • Long Name:
    Interleukin 13 Receptor alpha 2
  • Entrez Gene IDs:
    3598 (Human); 16165 (Mouse)
  • Alternate Names:
    cancer/testis antigen 19; CD213a2 antigen; CD213a2; CT19; IL-13 R alpha 2; IL-13 receptor subunit alpha-2; IL13BP; IL13R alpha 2; IL-13R subunit alpha-2; IL13R; IL-13R; IL13RA2; IL-13Ra2; IL-13R-alpha-2; interleukin 13 binding protein; interleukin 13 receptor alpha 2 chain; interleukin 13 receptor, alpha 2; interleukin-13 receptor subunit alpha-2; Interleukin-13-binding protein
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Goat IgG APC-conjugated Antibody

Ctrl IC108A 2  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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