Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 133617
View all IL-17RA/IL-17R Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-17 R
Leu33-Trp320
Accession # Q96F46

Specificity

Detects human IL-17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL‑17 R, rmIL‑17B R, or recombinant human IL‑17B R is observed. In Western blots, no cross-reactivity with rmIL-17 R is observed

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IL-17RA/IL-17R APC-conjugated Antibody

Detection of IL-17 R antibody in K562 Human Cell Line antibody by Flow Cytometry.

Detection of IL-17 R in K562 Human Cell Line by Flow Cytometry.

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 R APC-conjugated Monoclonal Antibody (Catalog # FAB177A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
IL-17 RA/IL-17 R Antibody Specificity is Shown by Flow Cytometry in Knockout Cell Line.

IL-17 RA/IL-17 R Specificity is Shown by Flow Cytometry in Knockout Cell Line.

IL-17 RA/IL-17 R knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 RA/IL-17 R APC-conjugated Monoclonal Antibody (Catalog # FAB177A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). No staining in the IL-17 RA/IL-17 R knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Applications for Human IL-17RA/IL-17R APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: K562 human chronic myelogenous leukemia cell line

Knockout Validated

IL-17 RA/IL-17 R is specifically detected in K562 myelogenous leukemia parental cell line but is not detectable in IL-17 RA/IL-17 R knockout K562 cell line.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-17RA/IL-17R

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. Human IL-17 R is a 120 kDa, 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. Within the ECD, human IL-17 R shares 72% aa sequence identity with mouse and rat IL-17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.

References

  1. Yao, Z. et al. (1997) Cytokine 9:794.
  2. Schwander, R. et al. (2000) J. Exp. Med. 191:1233.

Long Name

Interleukin 17 Receptor

Alternate Names

CD217, Cdw217, IL-17 R, IL-17 RA, IL17RA

Entrez Gene IDs

23765 (Human); 16172 (Mouse); 312679 (Rat); 486759 (Canine); 101925679 (Cynomolgus Monkey)

Gene Symbol

IL17RA

UniProt

Q96F46

Additional IL-17RA/IL-17R Products

Product Documents for Human IL-17RA/IL-17R APC-conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human IL-17RA/IL-17R APC-conjugated Antibody

For research use only

Related Research Areas

Cytokines and Receptors Research

Citations for Human IL-17RA/IL-17R APC-conjugated Antibody

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