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Human IL-18 BPa Quantikine ELISA Kit

R&D Systems | Catalog # DBP180

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (10 µL)

Sensitivity

7.52 pg/mL

Assay Range

26.6-1700 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
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Product Summary for Human IL-18 BPa Quantikine ELISA Kit

The Quantikine Human IL-18 BPa Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-18 BPa in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human IL-18 BPa and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-18 BPa. Results obtained using natural human IL-18 BPa showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-18 BPa.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human IL-18 BPa

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of a known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to determine inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.

Cell Culture Supernates, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 123 404 789 136 434 841
Standard Deviation 5.50 9.49 21.1 9.47 32.8 59.1
CV% 4.5 2.3 2.7 7.0 7.6 7.0

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 109 368 740 115 362 692
Standard Deviation 5.73 16.4 15.8 13.5 29.1 48.3
CV% 5.3 4.5 2.1 11.7 8.0 7.0

Recovery for Human IL-18 BPa Quantikine ELISA Kit

The recovery of human IL-18 BPa spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 106 98-114
EDTA Plasma (n=4) 106 93-113
Heparin Plasma (n=4) 105 92-119
Serum (n=4) 102 92-113

Linearity

To assess the linearity of the assay, samples containing high concentrations of human IL-18 BPa were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human IL-18 BPa ELISA Linearity

Scientific Data Images for Human IL-18 BPa Quantikine ELISA Kit

Human IL-18 BPa ELISA Calibrator Diluent RD5-18 Standard Curve

Human IL-18 BPa ELISA Calibrator Diluent RD5-18 Standard Curve

Human IL-18 BPa ELISA Calibrator Diluent RD6-68 Standard Curve

Human IL-18 BPa ELISA Calibrator Diluent RD6-68 Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-18 BPa

Interleukin 18 binding proteins are secreted glycoproteins which antagonize IL-18 activity by binding IL-18 and blocking its biological activity. Alternative splicing of the IL-18 BP gene produces at least four isoforms that differ in affinity for IL-18. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig-like domain.

Long Name

Interleukin 18 Binding Protein Isoform a

Alternate Names

IL18 BPa, IL18BP

Entrez Gene IDs

10068 (Human); 84388 (Rat); 107126419 (Cynomolgus Monkey)

Gene Symbol

IL18BP

Additional IL-18 BPa Products

Product Documents for Human IL-18 BPa Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IL-18 BPa Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Related Research Areas

IL-1 Family

Citations for Human IL-18 BPa Quantikine ELISA Kit

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  • Human IL-18 BPa Quantikine ELISA Kit
    Name: Milos Lazic
    Sample Tested: Kupffer cells
    Verified Customer | Posted 05/08/2018
    Very good ELISA. Have been using it only in cell supernatants. Linear standard curve, easy to calculate IL-18 concentrations. Appropriate for concentration response curves. Used it instead of HTRF, due to higher sensitivity!
    Human IL-18 BPa Quantikine ELISA Kit DBP180

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Protocols

View specific protocols for Human IL-18 BPa Quantikine ELISA Kit (DBP180):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL of Conjugate to each well.
  9.   For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    For Cell Culture Supernate/Urine Samples: Cover with a new plate sealer, and incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.
    10. 50 µL Stop Solution
  10. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Human IL-18 BPa Quantikine ELISA Kit

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  • Q: Can DPB180 be used to detect IL-18 BPa when complexed with IL-18?

    A: DBP180 was not designed to detect IL-18BPa when in complex with IL-18. Internal testing suggests IL-18 interferes with detection of IL-18 BPa at concentrations greater than 1.7 ng/ml. 

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Associated Pathways

IL-1 Family Signaling Pathways IL-1 Family Signaling Pathway Thumbnail