< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference observed with 1 or more available related molecules.
QC27, Quantikine Immunoassay Control Set 860 for Human IL-18 BPa - Please Inquire
The Quantikine Human IL-18 BPa Immunoassay is a 4.5 hour solid phase ELISA
designed to measure IL-18 BPa in cell culture supernates, cell lysates,
serum, and plasma. It contains NS0-expressed recombinant human IL-18 BPa
and antibodies raised against the recombinant factor. This immunoassay
has been shown to accurately quantitate recombinant human IL-18 BPa.
Results obtained using natural human IL-18 BPa showed linear curves that
were parallel to the standard curves obtained using the Quantikine kit
standards. These results indicate that this kit can be used to
determine relative mass values for natural human IL-18 BPa.
Intra-Assay Precision (Precision within an assay) Three samples of a known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays
to determine inter-assay precision. Assays were performed by at least
three technicians using two lots of kit components.
Serum, EDTA Plasma, Heparin Plasma
Cell Culture Supernates, Urine
The recovery of human IL-18 BPa spiked to levels throughout the range
of the assay in various matrices was evaluated.
Average % Recovery
Cell Culture Supernates (n=4)
EDTA Plasma (n=4)
Heparin Plasma (n=4)
To assess the linearity of the assay, samples containing high concentrations of human IL-18 BPa were serially diluted with the
appropriate Calibrator Diluent to produce samples with values within
the dynamic range of the assay.
Preparation and Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-18 BPa
Interleukin 18 binding proteins are secreted glycoproteins which antagonize IL-18 activity by binding IL-18 and blocking its biological activity. Alternative splicing of the IL-18 BP gene produces at least four isoforms that differ in affinity for IL-18. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig-like domain.
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
Prepare all reagents, standard dilutions, and samples as directed in the product insert.
Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
100 µL Assay Diluent
Add 100 µL of Assay Diluent to each well.
50 µL Standard, Control, or Sample
Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
200 µL Conjugate
Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
Aspirate and wash 4 times.
200 µL Substrate Solution
Add 200 µL of Conjugate to each well.
For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 30 minutes. PROTECT FROM LIGHT. For Cell Culture Supernate/Urine Samples: Cover with a new plate sealer, and incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.
50 µL Stop Solution
Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.