Pro-IL-18 (pro-Interleukin 18; also known as pro-IGIF and pro-IL-1 gamma ) is a 24 kDa member of the IL-1 family of molecules. It is widely expressed, being produced by keratinocytes, intestinal epithelium, T cells, macrophages and osteoblasts. Human Pro-IL-18 is 193 amino acids (aa) in length. Although mature IL-18 induces IFN-gamma secretion by NK and T cells, Pro-IL-18 appears to have little intrinsic activity. Generally, active IL-18 is considered to arise from caspase-1 cleavage of Pro-IL-18 between Asp36-Tyr37. This generates an 18 kDa mature C-terminal fragment, and a 4 kDa (predicted) N-terminal prosegment that runs at 6 kDa in SDS-PAGE. Other proteases are known to process Pro-IL-18. Caspase-3 cleavage after Asp68 generates an inactive 14 kDa mature segment, Merpin beta -subunit cleavage after Asn52 generates a marginally active 17 kDa mature segment, while parasite Cys protease cleavage after Val47 generates an inactive 17 kDa mature molecule. One splice variant shows a deletion of aa 27-30. Over aa 2-36, human Pro-IL-18 shares 63% aa identity with mouse Pro-IL-18.
Human IL‑18/IL‑1F4 Propeptide PE‑conjugated Antibody
R&D Systems | Catalog # IC646P
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 74801
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Product Specifications
Immunogen
E. coli-derived recombinant human IL‑18/IL‑1F4
Accession # Q14116
Accession # Q14116
Specificity
Detects the pro region of human IL-18/IL-1F4 in direct ELISAs and Western blots. Does not cross‑react with recombinant human IL-18, recombinant mouse IL-18 or recombinant rat IL-18.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human IL‑18/IL‑1F4 Propeptide PE‑conjugated Antibody
Detection of IL‑18/IL‑1F4 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) either untreated (upper panel) or treated with LPS for 24 hours (lower panel) were stained with Mouse Anti-Human IL-18/IL-1F4 PE-conjugated Monoclonal Antibody (Catalog # IC646P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of IL‑18/IL‑1F4 in THP-1 Human Cell Line by Flow Cytometry.
THP-1 human monocytic leukemia cell line was stained with Mouse Anti-Human IL-18/IL-1F4 PE-conjugated Monoclonal Antibody (Catalog # IC646P, filled histogram) or isotype control antibody (IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.Applications for Human IL‑18/IL‑1F4 Propeptide PE‑conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Human THP-1 cell line, or peripheral blood mononuclear cells (PBMCs) treated with LPS were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: Human THP-1 cell line, or peripheral blood mononuclear cells (PBMCs) treated with LPS were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Reviewed Applications
Read 3 reviews rated 3 using IC646P in the following applications:
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-18/IL-1F4
Long Name
Interleukin 18
Alternate Names
IGIF, IL-1F4, IL-1g, IL18
Gene Symbol
IL18
UniProt
Additional IL-18/IL-1F4 Products
Product Documents for Human IL‑18/IL‑1F4 Propeptide PE‑conjugated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human IL‑18/IL‑1F4 Propeptide PE‑conjugated Antibody
For research use only
Citations for Human IL‑18/IL‑1F4 Propeptide PE‑conjugated Antibody
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Customer Reviews for Human IL‑18/IL‑1F4 Propeptide PE‑conjugated Antibody (3)
3 out of 5
3 Customer Ratings
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Application: Flow CytometrySample Tested: Blood mononuclear cells (PBMCs)Species: HumanVerified Customer | Posted 07/06/2025
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Application: ELISASample Tested: Peripheral blood mononuclear cells (PBMCs)Species: HumanVerified Customer | Posted 05/14/2022
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Application: Flow CytometrySample Tested: Blood mononuclear cells (PBMCs)Species: HumanVerified Customer | Posted 03/08/2022
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Protocols
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