Intracellular Staining by Flow Cytometry
|Detection of IL‑19 in Human Peripheral Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were treated with LPS and stained with Mouse Anti-Human IL‑19 PE‑conjugated Monoclonal Antibody (Catalog # IC1035P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Intracellular Molecules.|
Human Interleukin 19 (IL-19) is a member of the IL-10 family of related cytokines. Its gene contains two alternate translation initiation sites, generating precursors of 215 amino acids (aa) and 177 aa, respectively. Both isoforms are processed to 17 kDa, 153 aa mature molecules. IL-19 contains seven helices and is secreted as a 28-32 kDa monomer. There are two potential N-linked glycosylation sites, both of which are likely utilized. Mature human IL-19 shares 69% aa sequence identity with the mature mouse homologue. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells. IL-19 expression is more widespread than initially thought and is now known to be secreted by keratinocytes, monocytes, astrocytes, endothelial cells, B cells, macrophages and tracheal epithelium. IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (IL-20 R alpha, also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R beta or IL-20 R2). This receptor complex is also shared by IL-20 and IL-24. Functionally, IL-19 appears to act often in an autocrine manner, with keratinocytes, macrophages and endothelium all producing and responding to IL-19. In general, IL-19 generates anti-inflammatory activities.