Human IL-1 beta /IL-1F2 PerCP-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human IL-1 beta /IL-1F2 in Western blots. Shows less than 5% cross-reactivity with recombinant mouse (rm) IL‑1 beta and rpIL‑1 beta and no cross-reactivity with rrIL‑1 beta, rmIL‑1 alpha, rhIL‑1ra, rmIL‑1ra, or rrIL‑1 alpha.
  • Source
    Monoclonal Mouse IgG1 Clone # 8516
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human IL-1 beta /IL-1F2
    aa 117-269
    Accession # P01584
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑1 beta /IL‑1F2 in LPS-treated Peripheral Blood Mononuclear Cell Monocytes by Flow Cytometry. Peripheral blood mononuclear cell monocytes, resting (open histogram) or treated with LPS (filled histogram), were stained with Mouse Anti-Human IL‑1 beta /IL‑1F2 PerCP‑conjugated Monoclonal Antibody (Catalog # IC201C) or isotype control antibody (Catalog # IC002C). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-1 beta/IL-1F2

IL-1 is a name that defines two 17 kDa pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), both of which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related single-chain polypeptides that share approximately 21% amino acid (aa) identity in human. Although IL-1 ( alpha and beta ) references two distinct molecules, the term IL-1 is also generally applied to an eleven-member protein family that exhibits an Ala-xxx-Asp motif in its primary structure (1,3). IL-1 alpha and beta are not redundant. IL-1 alpha is constitutively expressed, contains an NLS, is active as a proform, and may present as a membrane-bound form due to myristoylation and glycosylation (4). Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, and they bind to the same cell surface receptor, the functional 80 kDa IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra, a third member of the IL-1 subfamily that also includes IL-33, functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (2,3). The human IL-1 beta cDNA encodes a 269 aa precursor that contains a 116 aa propeptide that is cleaved intracellularly by the inflammasome-associated cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate an active 153 aa cytokine (3,5,6). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67-78% aa sequence identity with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.

  • References:
    1. Dinarello, C.A. (2013) Semin. Immunol. 25:389.
    2. Palomo, J. et al. (2015) Cytokine. 76:25.
    3. Garlanda, C. et al. (2013) Immunity 39:1003.
    4. Rider, P. et al. (2013)  Semin. Immunol. 25:430.
    5. Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. USA 81:7907.
    6. Afonia, I.S. et al.  (2015)  Immunity 42:991.
  • Long Name:
    Interleukin 1 beta
  • Entrez Gene IDs:
    3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
  • Alternate Names:
    catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PerCP-conjugated Antibody

Ctrl IC002C  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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