Human IL-1 beta /IL-1F2 PerCP-conjugated Antibody

Catalog # Availability Size / Price Qty
IC201C
Detection of IL‑1 beta /IL‑1F2 in LPS-treated Peripheral Blood Mononuclear Cell Monocytes by Flow Cytometry.
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Human IL-1 beta /IL-1F2 PerCP-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-1 beta /IL-1F2 in Western blots. Shows less than 5% cross-reactivity with recombinant mouse (rm) IL‑1 beta and rpIL‑1 beta and no cross-reactivity with rrIL‑1 beta, rmIL‑1 alpha, rhIL‑1ra, rmIL‑1ra, or rrIL‑1 alpha.
Source
Monoclonal Mouse IgG1 Clone # 8516
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL-1 beta /IL-1F2
aa 117-269
Accession # P01584
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL-1 beta /IL-1F2 antibody in LPS-treated Peripheral Blood Mononuclear Cell Monocytes antibody by Flow Cytometry. View Larger

Detection of IL‑1 beta /IL‑1F2 in LPS-treated Peripheral Blood Mononuclear Cell Monocytes by Flow Cytometry. Peripheral blood mononuclear cell monocytes, resting (open histogram) or treated with LPS (filled histogram), were stained with Mouse Anti-Human IL-1 beta /IL-1F2 PerCP-conjugated Monoclonal Antibody (Catalog # IC201C) or isotype control antibody (Catalog # IC002C). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

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