Human IL-1 beta /IL-1F2 (Research Grade Canakinumab Biosimilar) Antibody

Recombinant Monoclonal Antibody For Research Use Only
Catalog # Availability Size / Price Qty
MAB10349-100
MAB10349-SP
Human IL-1 beta /IL-1F2 (Research Grade Canakinumab Biosimilar) Antibody in Intracellular Staining by Flow Cytometry
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Human IL-1 beta /IL-1F2 (Research Grade Canakinumab Biosimilar) Antibody Summary

Species Reactivity
Human
Specificity
Detects Human IL‑1 beta /IL‑1F2 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Canakinumab. This product is for research use only.
Source
Recombinant Monoclonal Human IgG1 Clone # Hu110
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human IL‑1 beta /IL‑1F2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of IL-1 beta / IL-1F2 in Human blood monocytes by Flow Cytometry. View Larger

Detection of IL-1 beta / IL-1F2 in Human blood monocytes by Flow Cytometry. Human peripheral blood monocytes, either (A) treated with 500 ng/mL LPS overnight, or (B) untreated, were stained with Human anti-Human IL-1 beta/IL-1F2 (Research Grade Canakinumab Biosimilar) Antibody (Catalog # MAB10349) followed by APC-conjugated anti-Human IgG secondary antibody (Catalog # F0135) and Mouse anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). To facilitate intracellular staining, cells were fixed with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

 Canakinumab is a biosimilar directed against human IL-1 beta.

Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Product Datasheets

FAQs

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Reconstitution Buffers

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