Human IL-1ra/IL-1F3 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
PDRA00B
SRA00B
DRA00B
Control Products Available
Human IL-1ra/IL-1F3 ELISA Calibrator Diluent RD5-33 (1X) Standard Curve
3 Images
Product Details
Citations (70)
FAQs
Supplemental Products
Reviews (4)

Human IL-1ra/IL-1F3 Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity
18.3 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human IL-1ra
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human IL-1ra Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-1ra in cell culture supernates, serum, and plasma. It contains E. coli-derived recombinant human IL-1ra as well as antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-1ra. Results obtained using natural human IL-1ra showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-1ra.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 66.9 607 1627 82.5 646 1663
Standard Deviation 4.88 30.2 60 9.04 51.4 111
CV% 7.3 5 3.7 11 8 6.7

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 64.9 538 1343 56.2 469 1243
Standard Deviation 3.67 22.1 55.7 5.79 31.9 70.4
CV% 5.6 4.1 4.1 10.3 6.8 5.7

Recovery

The recovery of IL-1ra spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 106 96-115
EDTA Plasma (n=4) 86 66-102
Heparin Plasma (n=4) 93 82-106
Serum (n=4) 90 81-99

Linearity

To assess the linearity of the assay, samples containing high concentrations of IL-1ra were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-1ra/IL-1F3 ELISA Linearity

Data Examples

Human IL-1ra/IL-1F3 ELISA Calibrator Diluent RD5-33 (1X) Standard Curve

Human IL-1ra/IL-1F3 ELISA Calibrator Diluent RD6-3 Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1ra/IL-1F3

Interleukin-1 receptor antagonist (IL-1ra; also known as IL-1F3) is a 22-25 kDa member of the IL-1 family of cytokines. Currently, there are 11 family members (IL-1F1-F11), nine of which form an IL-1 gene cluster on human Ch2 (1-3). Each IL-1 family member contains an IL-1 fold. This fold is generated by 12 packed beta -sheets that interact to form a beta -trefoil structure. Little amino acid (aa) homology is required to achieve this structure, and this explains the low aa identity among IL-1 family members. IL-1ra is a pure cytokine receptor antagonist that has no signal transduction-initiating activity (4). It is an acute phase protein that exists to dampen inflammation. IL-1( beta ) is initially produced by monocytes in response to a variety of stimuli. Circulating IL-1 then binds to widely expressed IL-1 type I receptors (IL-1 RI) and initiates a number of pro-inflammatory events. On endothelial cells (EC), IL-1 induces PGE2 and IL-6 release, generating fever, thrombocytosis, and hepatic acute phase protein production. In synovial joints, IL-1 induces chondrocyte NO production, an event that leads to reduced collagen synthesis and chondrocyte apoptosis. Finally, IL-1 increases neutrophil counts, both in blood and tissue, and thus is able to promote a pro-inflammatory environment in multiple locations (5-8). IL-1ra blocks IL-1 action through competitive inhibition. More correctly, although IL-1ra fills the IL-1 binding site in IL-1 RI, it is also unable to orchestrate the creation of a signal-transducing IL-1 RI:IL-1 R Accessory protein (IL-1 R AcP) heterodimer complex. Effective IL-1ra concentrations are generally 100-fold greater than local IL-1 concentrations. This is because the IL-1ra half-life is but 6 minutes, and very few IL-1 type I receptors need to be engaged by IL-1 to elicit a cellular response (5, 7, 9). 

Human IL-1ra is synthesized as a 177 aa precursor that contains a 25 aa signal sequence and a 152 aa mature region (10, 11). Although it contains an IL-1 cytokine fold, it apparently lacks two structural motifs that allow for activation of the IL-1 receptor heterodimer. First, and following binding to IL-1 RI, the presence of Ile 51-His 54 and Lys 145 of the mature molecule preclude recruitment of IL-1 R AcP. Second, there is no identifiable C-terminal lectin segment that is hypothesized to help recruit an accessory signaling component (1, 12, 13). Mature human IL-1ra is 77% and 82% aa identical to mouse and canine IL-1ra, respectively, and human IL-1ra inhibits IL-1 activity on mouse cells (10). A number of cell types express IL-1ra, including monocytes (11), Sertoli cells (14), hepatocytes (15), adipocytes (16), synovial fibroblasts (17), mast cells (18), pancreatic beta -cells (19), and intestinal epithelial cells (20). There are at least three intracellular IL-1ra isoforms (icIL-1ra1, 2, and 3). All show N-terminal variation, and all contain amino acids 35-177 of the secreted precursor (21-23). Intracellular IL-1ra1 is of particular interest, because it is reported to be "secreted" by endothelial cells and binds to the IL-1 RI in an antagonist fashion (23-25). Intracellular IL-1ra1 is 159 aa in length and shows a 3 aa substitution for the first 21 aa's of the signal sequence of IL-1ra (21).

Long Name:
Interleukin 1 Receptor Antagonist
Entrez Gene IDs:
3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine)
Alternate Names:
DIRA; ICIL-1ra; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human IL-1ra/IL-1F3 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

70 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Quartz Dust Exposure Affects NLRP3 Inflammasome Activation and Plasma Levels of IL-18 and IL-1Ra in Iron Foundry Workers
    Authors: A Hedbrant, L Andersson, IL Bryngelsso, D Eklund, H Westberg, E Särndahl, A Persson
    Mediators Inflamm., 2020;2020(0):8490908.
    Species: Human
    Sample Types: Plasma
  2. Interferon and anti-TNF therapies differentially modulate amygdala reactivity which predicts associated bidirectional changes in depressive symptoms
    Authors: KA Davies, E Cooper, V Voon, J Tibble, M Cercignani, NA Harrison
    Mol. Psychiatry, 2020;0(0):.
    Species: Human
    Sample Types: Plasma
  3. Monounsaturated fat rapidly induces hepatic gluconeogenesis and whole-body insulin resistance
    Authors: T Sarabhai, S Kahl, J Szendroedi, DF Markgraf, OP Zaharia, C Barosa, C Herder, F Wickrath, P Bobrov, JH Hwang, JG Jones, M Roden
    JCI Insight, 2020;5(10):.
    Species: Human
    Sample Types: Serum
  4. All-source and source-specific air pollution and 10-year diabetes Incidence: Total effect and mediation analyses in the Heinz Nixdorf recall study
    Authors: S Lucht, F Hennig, S Moebus, S Ohlwein, C Herder, B Kowall, KH Jöckel, B Hoffmann
    Environ Int, 2020;136(0):105493.
    Species: Human
    Sample Types: Serum
  5. Air pollution and diabetes-related biomarkers in non-diabetic adults: A pathway to impaired glucose metabolism?
    Authors: S Lucht, F Hennig, S Moebus, D Führer-Sak, C Herder, KH Jöckel, B Hoffmann,
    Environ Int, 2019;124(0):370-392.
    Species: Human
    Sample Types:
  6. Acute transient cognitive dysfunction and acute brain injury induced by systemic inflammation occur by dissociable IL-1-dependent mechanisms
    Authors: DT Skelly, ÉW Griffin, CL Murray, S Harney, C O'Boyle, E Hennessy, MA Dansereau, A Nazmi, L Tortorelli, JN Rawlins, DM Bannerman, C Cunningham
    Mol. Psychiatry, 2019;24(10):1533-1548.
    Species: Mouse
    Sample Types: Plasma
  7. Prognostic impact of Interleukin-1 receptor antagonist in patients with documented coronary artery disease
    Authors: N Schofer, S Ludwig, N Rübsamen, R Schnabel, KJ Lackner, HJ Ruprecht, C Bickel, U Landmesser, S Blankenber, T Zeller
    Int. J. Cardiol., 2018;0(0):.
    Species: Human
    Sample Types: Plasma
  8. Macrophage-derived IL-1?/NF-?B signaling mediates parenteral nutrition-associated cholestasis
    Authors: KC El Kasmi, PM Vue, AL Anderson, MW Devereaux, S Ghosh, N Balasubram, SA Fillon, C Dahrenmoel, A Allawzi, C Woods, S McKenna, CJ Wright, L Johnson, A D'Alessand, JA Reisz, E Nozik-Gray, FJ Suchy, RJ Sokol
    Nat Commun, 2018;9(1):1393.
    Species: Human
    Sample Types: Serum
  9. Fatal septic shock caused by Paracoccidioides brasiliensis phylogenetic species S1 in a young immunocompetent patient: a case report
    Authors: PM Macedo, R Almeida-Pa, MA Almeida, RA Coelho, MA Oliveira F, DM Medeiros, A Gomes-Silv, JR Lima, AM Da-Cruz, RM Zancopé-Ol, ACFD Valle
    Rev. Soc. Bras. Med. Trop., 2018;51(1):111-114.
    Species: Human
    Sample Types: Plasma
  10. Association between pro- and anti-inflammatory cytokines and depressive symptoms in patients with diabetes-potential differences by diabetes type and depression scores
    Authors: C Herder, A Schmitt, F Budden, A Reimer, B Kulzer, M Roden, T Haak, N Hermanns
    Transl Psychiatry, 2018;7(11):1.
    Species: Human
    Sample Types: Serum
  11. Aggression, impulsivity and inflammatory markers as risk factors for suicidal behavior
    Authors: W Coryell, H Wilcox, SJ Evans, GN Pandey, L Jones-Bran, F Dickerson, R Yolken
    J Psychiatr Res, 2018;106(0):38-42.
    Species: Human
    Sample Types: Plasma
  12. IL-1 signaling mediates intrauterine inflammation and chorio-decidua neutrophil recruitment and activation
    Authors: P Presicce, CW Park, P Senthamara, S Bhattachar, C Jackson, F Kong, CM Rueda, E DeFranco, LA Miller, DA Hildeman, N Salomonis, CA Chougnet, AH Jobe, SG Kallapur
    JCI Insight, 2018;3(6):.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Plasma
  13. A short report on the effect of decreased incubation time on the architectural profile of autologous conditioned serum (ACS)
    Authors: A Barreto
    Cytokine, 2017;0(0):.
    Species: Human
    Sample Types: Serum
  14. Interleukin-1 receptor antagonist, a biomarker of response to anti-TB treatment in HIV/TB co-infected patients
    Authors: J Nouhin, P Pean, Y Madec, MF Chevalier, C Didier, L Borand, FX Blanc, D Scott-Alga, D Laureillar, L Weiss
    J. Infect, 2017;0(0):.
    Species: Human
    Sample Types: Plasma
  15. Inflammation and Arterial Stiffness in Chronic Kidney Disease: Findings From the CRIC Study
    Authors: E Peyster, J Chen, HI Feldman, AS Go, J Gupta, N Mitra, Q Pan, A Porter, M Rahman, D Raj, M Reilly, MR Wing, W Yang, RR Townsend,
    Am. J. Hypertens., 2017;30(4):400-408.
    Species: Human
    Sample Types: Plasma
  16. Genetic Regulation of Adipose Gene Expression and Cardio-Metabolic Traits
    Authors: M Civelek, Y Wu, C Pan, CK Raulerson, A Ko, A He, C Tilford, NK Saleem, A Stan?áková, LJ Scott, C Fuchsberge, HM Stringham, AU Jackson, N Narisu, PS Chines, KS Small, J Kuusisto, BW Parks, P Pajukanta, T Kirchgessn, FS Collins, PS Gargalovic, M Boehnke, M Laakso, KL Mohlke, AJ Lusis
    Am. J. Hum. Genet, 2017;100(3):428-443.
    Species: Human
    Sample Types: Plasma
  17. Intranasal Administration of Interleukin-1 Receptor Antagonist in a Transient Focal Cerebral Ischemia Rat Model
    Authors: JH Lee, EH Kam, JM Kim, SY Kim, EJ Kim, SY Cheon, BN Koo
    Biomol Ther (Seoul), 2017;25(2):149-157.
    Species: Rat
    Sample Types: Serum
  18. Downregulation of tumor suppressive microRNAs in vivo in dense breast tissue of postmenopausal women
    Authors: A Abrahamsso, A Capodanno, A Rzepecka, C Dabrosin
    Oncotarget, 2017;8(54):92134-92142.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Stability of cytokines, chemokines and soluble activation markers in unprocessed blood stored under different conditions
    Cytokine, 2016;84(0):17-24.
    Species: Human
    Sample Types: Plasma
  20. Lipopolysaccharide-Induced Chorioamnionitis Promotes IL-1-Dependent Inflammatory FOXP3+ CD4+ T Cells in the Fetal Rhesus Macaque
    Authors: CM Rueda, P Presicce, CM Jackson, LA Miller, SG Kallapur, AH Jobe, CA Chougnet
    J Immunol, 2016;0(0):.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Plasma
  21. A novel approach for emerging and antibiotic resistant infections: Innate defense regulators as an agnostic therapy
    Authors: JR North, S Takenaka, A Rozek, A Kielczewsk, S Opal, LA Morici, BB Finlay, CJ Schaber, R Straube, O Donini
    J. Biotechnol., 2016;226(0):24-34.
    Species: Primate
    Sample Types: Plasma
  22. Association study of arcuate nucleus neuropeptide Y neuron receptor gene variation and serum NPY levels in clozapine treated patients with schizophrenia
    Authors: E Leinonen
    Eur. Psychiatry, 2016;40(0):13-19.
    Species: Human
    Sample Types: Serum
  23. Differential Patterns and Determinants of Cardiac Autonomic Nerve Dysfunction during Endotoxemia and Oral Fat Load in Humans.
    Authors: Ziegler D, Strom A, Strassburger K, Nowotny B, Zahiragic L, Nowotny P, Carstensen-Kirberg M, Herder C, Szendroedi J, Roden M
    PLoS ONE, 2015;10(4):e0124242.
    Species: Human
    Sample Types: Serum
  24. IL-1 receptor antagonist reduces endotoxin-induced airway inflammation in healthy volunteers.
    Authors: Hernandez M, Mills K, Almond M, Todoric K, Aleman M, Zhang H, Zhou H, Peden D
    J Allergy Clin Immunol, 2015;135(2):379-85.
    Species: Human
    Sample Types: Plasma
  25. Association between Inflammation and Cardiac Geometry in Chronic Kidney Disease: Findings from the CRIC Study.
    Authors: Gupta J, Dominic E, Fink J, Ojo A, Barrows I, Reilly M, Townsend R, Joffe M, Rosas S, Wolman M, Patel S, Keane M, Feldman H, Kusek J, Raj D
    PLoS ONE, 2015;10(4):e0124772.
    Species: Human
    Sample Types: Plasma
  26. The effect of a Diabetes-Specific Cognitive Behavioral Treatment Program (DIAMOS) for patients with diabetes and subclinical depression: results of a randomized controlled trial.
    Authors: Hermanns N, Schmitt A, Gahr A, Herder C, Nowotny B, Roden M, Ohmann C, Kruse J, Haak T, Kulzer B
    Diabetes Care, 2015;38(4):551-60.
    Species: Human
    Sample Types: Serum
  27. Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma.
    Authors: Hengartner N, Fiedler J, Schrezenmeier H, Huber-Lang M, Brenner R
    PLoS ONE, 2015;10(1):e0116772.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. The expression of cytokines and chemokines in the blood of patients with severe weight loss from anorexia nervosa: an exploratory study.
    Authors: Pisetsky D, Trace S, Brownley K, Hamer R, Zucker N, Roux-Lombard P, Dayer J, Bulik C
    Cytokine, 2014;69(1):110-5.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Race modifies the association between adiposity and inflammation in patients with chronic kidney disease: findings from the chronic renal insufficiency cohort study.
    Authors: Wing M, Yang W, Teal V, Navaneethan S, Tao K, Ojo A, Guzman N, Reilly M, Wolman M, Rosas S, Cuevas M, Fischer M, Lustigova E, Master S, Xie D, Appleby D, Joffe M, Kusek J, Feldman H, Raj D
    Obesity (Silver Spring), 2014;22(5):1359-66.
    Species: Human
    Sample Types: Plasma
  30. Proteomic analysis of saliva in HIV-positive heroin addicts reveals proteins correlated with cognition.
    Authors: Dominy, Stephen, Brown, Joseph N, Ryder, Mark I, Gritsenko, Marina, Jacobs, Jon M, Smith, Richard
    PLoS ONE, 2014;9(4):e89366.
    Species: Human
    Sample Types: Saliva
  31. Patterns of obesity development before the diagnosis of type 2 diabetes: the Whitehall II cohort study.
    Authors: Vistisen D, Witte D, Tabak A, Herder C, Brunner E, Kivimaki M, Faerch K
    PLoS Med, 2014;11(2):e1001602.
    Species: Human
    Sample Types: Serum
  32. The multifaceted effects of polysaccharides isolated from Dendrobium huoshanense on immune functions with the induction of interleukin-1 receptor antagonist (IL-1ra) in monocytes.
    Authors: Lin J, Chang Y, Yang W, Yu A, Wong C
    PLoS ONE, 2014;9(4):e94040.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Role of diacylglycerol activation of PKCtheta in lipid-induced muscle insulin resistance in humans.
    Authors: Szendroedi J, Yoshimura T, Phielix E, Koliaki C, Marcucci M, Zhang D, Jelenik T, Muller J, Herder C, Nowotny P, Shulman G, Roden M
    Proc Natl Acad Sci U S A, 2014;111(26):9597-602.
    Species: Human
    Sample Types: Plasma
  34. The impact of concomitant pulmonary infection on immune dysregulation in Pneumocystis jirovecii pneumonia.
    Authors: Chou C, Lin F, Tsai H, Chang S
    BMC Pulm Med, 2014;14(0):182.
    Species: Human
    Sample Types: BALF
  35. beta-glucan induces reactive oxygen species production in human neutrophils to improve the killing of Candida albicans and Candida glabrata isolates from vulvovaginal candidiasis.
    Authors: Bonfim-Mendonca P, Ratti B, Godoy J, Negri M, Lima N, Fiorini A, Hatanaka E, Consolaro M, de Oliveira Silva S, Svidzinski T
    PLoS ONE, 2014;9(9):e107805.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. Inflammation induced by MMP-9 enhances tumor regression of experimental breast cancer.
    Authors: Leifler K, Svensson S, Abrahamsson A, Bendrik C, Robertson J, Gauldie J, Olsson A, Dabrosin C
    J Immunol, 2013;190(8):4420-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. Association between traffic-related air pollution, subclinical inflammation and impaired glucose metabolism: results from the SALIA study.
    Authors: Teichert T, Vossoughi M, Vierkotter A, Sugiri D, Schikowski T, Schulte T, Roden M, Luckhaus C, Herder C, Kramer U
    PLoS ONE, 2013;8(12):e83042.
    Species: Human
    Sample Types: Plasma
  38. Opium addiction increases interleukin 1 receptor antagonist (IL-1Ra) in the coronary artery disease patients.
    Authors: Saadat, Habiboll, Ziai, Seyed Al, Ghanemnia, Maryam, Namazi, Mohammad, Safi, Morteza, Vakili, Hosein, Dabbagh, Ali, Gholami, Omid
    PLoS ONE, 2012;7(9):e44939.
    Species: Human
    Sample Types: Plasma
  39. Altered monocyte activation markers in Tourette's syndrome: a case-control study.
    Authors: Matz J, Krause DL, Dehning S, Riedel M, Gruber R, Schwarz MJ, Muller N
    BMC Psychiatry, 2012;12(0):29.
    Species: Human
    Sample Types: Serum
  40. Time-course of changes in inflammatory response after whole-body cryotherapy multi exposures following severe exercise.
    Authors: Pournot H, Bieuzen F, Louis J, Fillard JR, Barbiche E, Hausswirth C
    PLoS ONE, 2011;6(7):e22748.
    Species: Human
    Sample Types: Plasma
  41. The detrimental effects of IFN-alpha on vasculogenesis in lupus are mediated by repression of IL-1 pathways: potential role in atherogenesis and renal vascular rarefaction.
    Authors: Thacker SG, Berthier CC, Mattinzoli D, Rastaldi MP, Kretzler M, Kaplan MJ
    J. Immunol., 2010;185(7):4457-69.
    Species: Human
    Sample Types: Serum
  42. Accelerated increase in serum interleukin-1 receptor antagonist starts 6 years before diagnosis of type 2 diabetes: Whitehall II prospective cohort study.
    Authors: Carstensen M, Herder C, Kivimaki M, Jokela M, Roden M, Shipley MJ, Witte DR, Brunner EJ, Tabak AG
    Diabetes, 2010;59(5):1222-7.
    Species: Human
    Sample Types: Serum
  43. Changes in cytokine levels during acute hyperinsulinemia in offspring of type 2 diabetic subjects.
    Authors: Ruotsalainen E, Stancakova A, Vauhkonen I, Salmenniemi U, Pihlajamaki J, Punnonen K, Laakso M
    Atherosclerosis, 2010;210(2):536-41.
    Species: Human
    Sample Types: Plasma
  44. SIRT1 mRNA expression may be associated with energy expenditure and insulin sensitivity.
    Authors: Rutanen J, Yaluri N, Modi S, Pihlajamaki J, Vanttinen M, Itkonen P, Kainulainen S, Yamamoto H, Lagouge M, Sinclair DA, Elliott P, Westphal C, Auwerx J, Laakso M
    Diabetes, 2010;59(4):829-35.
    Species: Human
    Sample Types: Plasma
  45. Toll-like receptor-mediated production of IL-1Ra is negatively regulated by GSK3 via the MAPK ERK1/2.
    Authors: Rehani K, Wang H, Garcia CA, Kinane DF, Martin M
    J. Immunol., 2009;182(1):547-53.
    Species: Human
    Sample Types: Cell Culture Supernates
  46. In vivo regulation of interleukin 1beta in patients with cryopyrin-associated periodic syndromes.
    Authors: Lachmann HJ, Lowe P, Felix SD, Rordorf C, Leslie K, Madhoo S, Wittkowski H, Bek S, Hartmann N, Bosset S, Hawkins PN, Jung T
    J. Exp. Med., 2009;206(5):1029-36.
    Species: Human
    Sample Types: Plasma
  47. IL-1 mediates pulmonary and systemic inflammatory responses to chorioamnionitis induced by lipopolysaccharide.
    Authors: Kallapur SG, Nitsos I, Moss TJ, Polglase GR, Pillow JJ, Cheah FC, Kramer BW, Newnham JP, Ikegami M, Jobe AH
    Am. J. Respir. Crit. Care Med., 2009;179(10):955-61.
    Species: Ovine
    Sample Types: Amniotic Fluid
  48. Validity of multiplex-based assays for cytokine measurements in serum and plasma from "non-diseased" subjects: comparison with ELISA.
    Authors: Dossus L, Becker S, Achaintre D, Kaaks R, Rinaldi S
    J. Immunol. Methods, 2009;350(1):125-32.
    Species: Human
    Sample Types: Serum
  49. RAS mutations affect tumor necrosis factor-induced apoptosis in colon carcinoma cells via ERK-modulatory negative and positive feedback circuits along with non-ERK pathway effects.
    Authors: Kreeger PK, Mandhana R, Alford SK, Haigis KM, Lauffenburger DA
    Cancer Res., 2009;69(20):8191-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
    Authors: Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G
    Transplantation, 2009;88(9):1109-16.
    Species: Human
    Sample Types: Urine
  51. Self-esteem levels and cardiovascular and inflammatory responses to acute stress.
    Authors: O'Donnell K, Brydon L, Wright CE, Steptoe A
    Brain Behav. Immun., 2008;0(0):.
    Species: Human
    Sample Types: Plasma
  52. Evidence for a pathogenetic role of interleukin-18 in cutaneous lupus erythematosus.
    Authors: Wang D, Drenker M, Eiz-Vesper B, Werfel T, Wittmann M
    Arthritis Rheum., 2008;58(10):3205-15.
    Species: Human
    Sample Types: Cell Culture Supernates
  53. Short communication: decreasing soluble CD30 and increasing IFN-gamma plasma levels are indicators of effective highly active antiretroviral therapy.
    Authors: Sadeghi M, Susal C, Daniel V, Naujokat C, Zimmermann R, Huth-Kuhne A, Opelz G
    AIDS Res. Hum. Retroviruses, 2007;23(7):886-90.
    Species: Human
    Sample Types: Plasma
  54. PRO 2000 elicits a decline in genital tract immune mediators without compromising intrinsic antimicrobial activity.
    Authors: Keller MJ, Guzman E, Hazrati E, Kasowitz A, Cheshenko N, Wallenstein S, Cole AL, Cole AM, Profy AT, Wira CR, Hogarty K, Herold BC
    AIDS, 2007;21(4):467-76.
    Species: Human
    Sample Types: Vaginal Lavage Fluid
  55. Decreased levels of peritoneal interleukin-1 receptor antagonist in patients with endometriosis and disease-related dysmenorrhea.
    Authors: Zhang X, Wen J, Deng L, Lin J
    Fertil. Steril., 2007;88(3):594-9.
    Species: Human
    Sample Types: Serum
  56. Regulation of hepatocyte growth factor secretion by fibroblasts in patients with acute lung injury.
    Authors: Quesnel C, Marchand-Adam S, Fabre A, Marchal-Somme J, Philip I, Lasocki S, Lecon V, Crestani B, Dehoux M
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;294(2):L334-43.
    Species: Human
    Sample Types: BALF
  57. P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells.
    Authors: Wilson HL, Varcoe RW, Stokes L, Holland KL, Francis SE, Dower SK, Surprenant A, Crossman DC
    Br. J. Pharmacol., 2007;151(1):115-27.
    Species: Human
    Sample Types: Cell Culture Supernates
  58. First in human phase I trial of 852A, a novel systemic toll-like receptor 7 agonist, to activate innate immune responses in patients with advanced cancer.
    Authors: Dudek AZ, Yunis C, Harrison LI, Kumar S, Hawkinson R, Cooley S, Vasilakos JP, Gorski KS, Miller JS
    Clin. Cancer Res., 2007;13(23):7119-25.
    Species: Human
    Sample Types: Serum
  59. Low concentration of interleukin-1beta induces FLICE-inhibitory protein-mediated beta-cell proliferation in human pancreatic islets.
    Authors: Maedler K, Schumann DM, Sauter N, Ellingsgaard H, Bosco D, Baertschiger R, Iwakura Y, Oberholzer J, Wollheim CB, Gauthier BR, Donath MY
    Diabetes, 2006;55(10):2713-22.
    Species: Human
    Sample Types: Cell Culture Supernates
  60. Effects of the active metabolite of leflunomide, A77 1726, on cytokine release and the MAPK signalling pathway in human rheumatoid arthritis synoviocytes.
    Authors: Vergne-Salle&lt;/LastName&gt;&lt;F P&lt;/Initial, Vergne-Salle P, Leger DY, Bertin P, Treves R, Beneytout JL, Liagre B
    Cytokine, 2005;31(5):335-48.
    Species: Human
    Sample Types: Cell Culture Supernates
  61. Circulating and synovial levels of IGF-I, cytokines, physical function and anthropometry differ in women awaiting total knee arthroplasty when compared to men.
    Authors: Pagura SM, Thomas SG, Woodhouse LJ, Ezzat S, Marks P
    J. Orthop. Res., 2005;23(2):397-405.
    Species: Human
    Sample Types: Synovial Fluid
  62. Experimental heatstroke in baboon: analysis of the systemic inflammatory response.
    Authors: Bouchama A, Ollivier V, Roberts G, Al Mohanna F, de Prost D, Eldali A, Saussereau E, El-Sayed R, Chollet-Martin S
    Shock, 2005;24(4):332-5.
    Species: Primate - Papio hamadryas (Hamadyras Baboon)
    Sample Types: Plasma
  63. Strong inflammatory cytokine response in male and strong anti-inflammatory response in female kidney transplant recipients with urinary tract infection.
    Authors: Sadeghi M, Daniel V, Naujokat C, Wiesel M, Hergesell O, Opelz G
    Transpl. Int., 2005;18(2):177-85.
    Species: Human
    Sample Types: Plasma
  64. Levels of soluble cytokine factors in temporomandibular joint effusions seen on magnetic resonance images.
    Authors: Kaneyama K, Segami N, Sun W, Sato J, Fujimura K
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2005;99(4):411-8.
    Species: Human
    Sample Types: Synovial Fluid
  65. Guiding the vaginal microbicide trials with biomarkers of inflammation.
    Authors: Fichorova RN
    J. Acquir. Immune Defic. Syndr., 2004;37(0):S184-93.
    Species: Human
    Sample Types: Vaginal Lavage Fluid
  66. CD14 is an acute-phase protein.
    Authors: Bas S, Gauthier BR, Spenato U, Stingelin S, Gabay C
    J. Immunol., 2004;172(7):4470-9.
    Species: Human
    Sample Types: Serum
  67. Effects of scaling and root planing on the amounts of interleukin-1 and interleukin-1 receptor antagonist and the mRNA expression of interleukin-1beta in gingival crevicular fluid and gingival tissues.
    Authors: Yoshinari N, Kawase H, Mitani A, Ito M, Sugiishi S, Matsuoka M, Shirozu N, Ishihara Y, Bito B, Hiraga M, Arakawa K, Noguchi T
    J. Periodont. Res., 2004;39(3):158-67.
    Species: Human
    Sample Types: Gingival Crevicular Fluid (GCF)
  68. Determinants of plasma leptin concentration in postmenopausal women.
    Authors: Brooks-Asplund EM, Tupper CE, Daun JM, Kenney WL, Cannon JG
    Mech. Ageing Dev., 2004;125(2):117-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  69. IL-1 beta, IL-1 receptor antagonist and soluble type II IL-1 receptor in synovial fluid of patients with temporomandibular disorders.
    Authors: Tominaga K, Habu M, Sukedai M, Hirota Y, Takahashi T, Fukuda J
    Arch. Oral Biol., 2004;49(6):493-9.
    Species: Human
    Sample Types: Synovial Fluid
  70. Impact of increased occlusal contact, interleukin-1 genotype, and periodontitis severity on gingival crevicular fluid IL-1beta levels.
    Authors: McDevitt MJ, Russell CM, Schmid MJ, Reinhardt RA
    J. Periodontol., 2003;74(9):1302-7.
    Species: Human
    Sample Types: Gingival Crevicular Fluid (GCF)

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs

ELISA Controls

Supplemental ELISA Products

Reviews for Human IL-1ra/IL-1F3 Quantikine ELISA Kit

Average Rating: 5 (Based on 4 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human IL-1ra/IL-1F3 Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human IL-1ra/IL-1F3 Quantikine ELISA Kit
By Anonymous on 02/28/2020
Application: Sample Tested: Cancer cell lysates

Human IL-1ra/IL-1F3 Quantikine ELISA Kit
By Anonymous on 02/27/2020
Application: Sample Tested: Plasma

Human IL-1ra/IL-1F3 Quantikine ELISA Kit
By Anonymous on 02/27/2020
Application: Sample Tested: Plasma

easy instructions, clear and reliable results


Human IL-1ra/IL-1F3 Quantikine ELISA Kit
By Anonymous on 05/01/2018
Application: Sample Tested: Cell Culture Media