Human IL-1ra/IL-1F3 Quantikine ELISA Kit

(51 citations)   
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
  • Sensitivity
    18.3 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human IL-1ra
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC25, Quantikine Immunoassay Control Set 810 for Human IL-1ra - Please Inquire
Product Summary
The Quantikine Human IL-1ra Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-1ra in cell culture supernates, serum, and plasma. It contains E. coli-derived recombinant human IL-1ra as well as antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-1ra. Results obtained using natural human IL-1ra showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-1ra.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean66.9607162782.56461663
Standard Deviation4.8830.2609.0451.4111
CV%7.353.71186.7

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean64.9538134356.24691243
Standard Deviation3.6722.155.75.7931.970.4
CV%5.64.14.110.36.85.7

Recovery

The recovery of IL-1ra spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 106 96-115
EDTA Plasma (n=4) 86 66-102
Heparin Plasma (n=4) 93 82-106
Serum (n=4) 90 81-99
Linearity
To assess the linearity of the assay, samples containing high concentrations of IL-1ra were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-1ra/IL-1F3 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-1ra/IL-1F3
IL-1ra (IL-1 receptor antagonist) is a widely expressed acute phase protein that serves to dampen inflammation. IL-1ra binds to both IL-1 RI and IL-1 RII. It competitively inhibits IL-1 (alpha or beta) binding to IL-1 RI and does not trigger recruitment of the accessory molecule IL-1 R AcP or activation of IL-1 RI signaling. IL-1ra blocks the proinflammatory actions of IL-1 including Prostaglandin E2 and IL-6 release from endothelial cells, fever generation, thrombocytosis, the production of hepatic acute phase proteins, and neutrophil expansion and infiltration.
    • Long Name
      Interleukin 1 Receptor Antagonist
    • Entrez Gene IDs
      3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine);
    • Alternate Names
      DIRA; ICIL-1RA; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4;
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    51 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. A short report on the effect of decreased incubation time on the architectural profile of autologous conditioned serum (ACS)
      Authors: A Barreto
      Cytokine, 2017;0(0):.
      Species: Human
      Sample Type: Serum
    2. Stability of cytokines, chemokines and soluble activation markers in unprocessed blood stored under different conditions
      Cytokine, 2016;84(0):17-24.
      Species: Human
      Sample Type: Plasma
    3. Lipopolysaccharide-Induced Chorioamnionitis Promotes IL-1-Dependent Inflammatory FOXP3+ CD4+ T Cells in the Fetal Rhesus Macaque
      Authors: CM Rueda, P Presicce, CM Jackson, LA Miller, SG Kallapur, AH Jobe, CA Chougnet
      J Immunol, 2016;0(0):.
      Species: Primate - Macaca mulatta (Rhesus Macaque)
      Sample Type: Plasma
    4. Association between Inflammation and Cardiac Geometry in Chronic Kidney Disease: Findings from the CRIC Study.
      Authors: Gupta J, Dominic E, Fink J, Ojo A, Barrows I, Reilly M, Townsend R, Joffe M, Rosas S, Wolman M, Patel S, Keane M, Feldman H, Kusek J, Raj D
      PLoS ONE, 2015;10(4):e0124772.
      Species: Human
      Sample Type: Plasma
    5. Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma.
      Authors: Hengartner N, Fiedler J, Schrezenmeier H, Huber-Lang M, Brenner R
      PLoS ONE, 2015;10(1):e0116772.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. The effect of a Diabetes-Specific Cognitive Behavioral Treatment Program (DIAMOS) for patients with diabetes and subclinical depression: results of a randomized controlled trial.
      Authors: Hermanns N, Schmitt A, Gahr A, Herder C, Nowotny B, Roden M, Ohmann C, Kruse J, Haak T, Kulzer B
      Diabetes Care, 2015;38(4):551-60.
      Species: Human
      Sample Type: Serum
    7. Differential Patterns and Determinants of Cardiac Autonomic Nerve Dysfunction during Endotoxemia and Oral Fat Load in Humans.
      Authors: Ziegler D, Strom A, Strassburger K, Nowotny B, Zahiragic L, Nowotny P, Carstensen-Kirberg M, Herder C, Szendroedi J, Roden M
      PLoS ONE, 2015;10(4):e0124242.
      Species: Human
      Sample Type: Serum
    8. IL-1 receptor antagonist reduces endotoxin-induced airway inflammation in healthy volunteers.
      Authors: Hernandez M, Mills K, Almond M, Todoric K, Aleman M, Zhang H, Zhou H, Peden D
      J Allergy Clin Immunol, 2015;135(2):379-85.
      Species: Human
      Sample Type: Plasma
    9. Role of diacylglycerol activation of PKCtheta in lipid-induced muscle insulin resistance in humans.
      Authors: Szendroedi J, Yoshimura T, Phielix E, Koliaki C, Marcucci M, Zhang D, Jelenik T, Muller J, Herder C, Nowotny P, Shulman G, Roden M
      Proc Natl Acad Sci U S A, 2014;111(26):9597-602.
      Species: Human
      Sample Type: Plasma
    10. Race modifies the association between adiposity and inflammation in patients with chronic kidney disease: findings from the chronic renal insufficiency cohort study.
      Authors: Wing M, Yang W, Teal V, Navaneethan S, Tao K, Ojo A, Guzman N, Reilly M, Wolman M, Rosas S, Cuevas M, Fischer M, Lustigova E, Master S, Xie D, Appleby D, Joffe M, Kusek J, Feldman H, Raj D
      Obesity (Silver Spring), 2014;22(5):1359-66.
      Species: Human
      Sample Type: Plasma
    11. The expression of cytokines and chemokines in the blood of patients with severe weight loss from anorexia nervosa: an exploratory study.
      Authors: Pisetsky D, Trace S, Brownley K, Hamer R, Zucker N, Roux-Lombard P, Dayer J, Bulik C
      Cytokine, 2014;69(1):110-5.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Proteomic analysis of saliva in HIV-positive heroin addicts reveals proteins correlated with cognition.
      Authors: Dominy, Stephen, Brown, Joseph N, Ryder, Mark I, Gritsenko, Marina, Jacobs, Jon M, Smith, Richard
      PLoS ONE, 2014;9(4):e89366.
      Species: Human
      Sample Type: Saliva
    13. Patterns of obesity development before the diagnosis of type 2 diabetes: the Whitehall II cohort study.
      Authors: Vistisen D, Witte D, Tabak A, Herder C, Brunner E, Kivimaki M, Faerch K
      PLoS Med, 2014;11(2):e1001602.
      Species: Human
      Sample Type: Serum
    14. The multifaceted effects of polysaccharides isolated from Dendrobium huoshanense on immune functions with the induction of interleukin-1 receptor antagonist (IL-1ra) in monocytes.
      Authors: Lin J, Chang Y, Yang W, Yu A, Wong C
      PLoS ONE, 2014;9(4):e94040.
      Species: Human
      Sample Type: Cell Culture Supernates
    15. beta-glucan induces reactive oxygen species production in human neutrophils to improve the killing of Candida albicans and Candida glabrata isolates from vulvovaginal candidiasis.
      Authors: Bonfim-Mendonca P, Ratti B, Godoy J, Negri M, Lima N, Fiorini A, Hatanaka E, Consolaro M, de Oliveira Silva S, Svidzinski T
      PLoS ONE, 2014;9(9):e107805.
      Species: Human
      Sample Type: Cell Culture Supernates
    16. The impact of concomitant pulmonary infection on immune dysregulation in Pneumocystis jirovecii pneumonia.
      Authors: Chou C, Lin F, Tsai H, Chang S
      BMC Pulm Med, 2014;14(0):182.
      Species: Human
      Sample Type: BALF
    17. Association between traffic-related air pollution, subclinical inflammation and impaired glucose metabolism: results from the SALIA study.
      Authors: Teichert T, Vossoughi M, Vierkotter A, Sugiri D, Schikowski T, Schulte T, Roden M, Luckhaus C, Herder C, Kramer U
      PLoS ONE, 2013;8(12):e83042.
      Species: Human
      Sample Type: Plasma
    18. Inflammation induced by MMP-9 enhances tumor regression of experimental breast cancer.
      Authors: Leifler K, Svensson S, Abrahamsson A, Bendrik C, Robertson J, Gauldie J, Olsson A, Dabrosin C
      J Immunol, 2013;190(8):4420-30.
      Species: Human
      Sample Type: Cell Culture Supernates
    19. Opium addiction increases interleukin 1 receptor antagonist (IL-1Ra) in the coronary artery disease patients.
      Authors: Saadat, Habiboll, Ziai, Seyed Al, Ghanemnia, Maryam, Namazi, Mohammad, Safi, Morteza, Vakili, Hosein, Dabbagh, Ali, Gholami, Omid
      PLoS ONE, 2012;7(9):e44939.
      Species: Human
      Sample Type: Plasma
    20. Altered monocyte activation markers in Tourette's syndrome: a case-control study.
      Authors: Matz J, Krause DL, Dehning S, Riedel M, Gruber R, Schwarz MJ, Muller N
      BMC Psychiatry, 2012;12(0):29.
      Species: Human
      Sample Type: Serum
    21. Time-course of changes in inflammatory response after whole-body cryotherapy multi exposures following severe exercise.
      Authors: Pournot H, Bieuzen F, Louis J, Fillard JR, Barbiche E, Hausswirth C
      PLoS ONE, 2011;6(7):e22748.
      Species: Human
      Sample Type: Plasma
    22. SIRT1 mRNA expression may be associated with energy expenditure and insulin sensitivity.
      Authors: Rutanen J, Yaluri N, Modi S, Pihlajamaki J, Vanttinen M, Itkonen P, Kainulainen S, Yamamoto H, Lagouge M, Sinclair DA, Elliott P, Westphal C, Auwerx J, Laakso M
      Diabetes, 2010;59(4):829-35.
      Species: Human
      Sample Type: Plasma
    23. Accelerated increase in serum interleukin-1 receptor antagonist starts 6 years before diagnosis of type 2 diabetes: Whitehall II prospective cohort study.
      Authors: Carstensen M, Herder C, Kivimaki M, Jokela M, Roden M, Shipley MJ, Witte DR, Brunner EJ, Tabak AG
      Diabetes, 2010;59(5):1222-7.
      Species: Human
      Sample Type: Serum
    24. The detrimental effects of IFN-alpha on vasculogenesis in lupus are mediated by repression of IL-1 pathways: potential role in atherogenesis and renal vascular rarefaction.
      Authors: Thacker SG, Berthier CC, Mattinzoli D, Rastaldi MP, Kretzler M, Kaplan MJ
      J. Immunol., 2010;185(7):4457-69.
      Species: Human
      Sample Type: Serum
    25. Changes in cytokine levels during acute hyperinsulinemia in offspring of type 2 diabetic subjects.
      Authors: Ruotsalainen E, Stancakova A, Vauhkonen I, Salmenniemi U, Pihlajamaki J, Punnonen K, Laakso M
      Atherosclerosis, 2010;210(2):536-41.
      Species: Human
      Sample Type: Plasma
    26. In vivo regulation of interleukin 1beta in patients with cryopyrin-associated periodic syndromes.
      Authors: Lachmann HJ, Lowe P, Felix SD, Rordorf C, Leslie K, Madhoo S, Wittkowski H, Bek S, Hartmann N, Bosset S, Hawkins PN, Jung T
      J. Exp. Med., 2009;206(5):1029-36.
      Species: Human
      Sample Type: Plasma
    27. Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
      Authors: Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G
      Transplantation, 2009;88(9):1109-16.
      Species: Human
      Sample Type: Urine
    28. Toll-like receptor-mediated production of IL-1Ra is negatively regulated by GSK3 via the MAPK ERK1/2.
      Authors: Rehani K, Wang H, Garcia CA, Kinane DF, Martin M
      J. Immunol., 2009;182(1):547-53.
      Species: Human
      Sample Type: Cell Culture Supernates
    29. IL-1 mediates pulmonary and systemic inflammatory responses to chorioamnionitis induced by lipopolysaccharide.
      Authors: Kallapur SG, Nitsos I, Moss TJ, Polglase GR, Pillow JJ, Cheah FC, Kramer BW, Newnham JP, Ikegami M, Jobe AH
      Am. J. Respir. Crit. Care Med., 2009;179(10):955-61.
      Species: Ovine
      Sample Type: Amniotic Fluid
    30. RAS mutations affect tumor necrosis factor-induced apoptosis in colon carcinoma cells via ERK-modulatory negative and positive feedback circuits along with non-ERK pathway effects.
      Authors: Kreeger PK, Mandhana R, Alford SK, Haigis KM, Lauffenburger DA
      Cancer Res., 2009;69(20):8191-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    31. Self-esteem levels and cardiovascular and inflammatory responses to acute stress.
      Authors: O'Donnell K, Brydon L, Wright CE, Steptoe A
      Brain Behav. Immun., 2008;0(0):.
      Species: Human
      Sample Type: Plasma
    32. Evidence for a pathogenetic role of interleukin-18 in cutaneous lupus erythematosus.
      Authors: Wang D, Drenker M, Eiz-Vesper B, Werfel T, Wittmann M
      Arthritis Rheum., 2008;58(10):3205-15.
      Species: Human
      Sample Type: Cell Culture Supernates
    33. PRO 2000 elicits a decline in genital tract immune mediators without compromising intrinsic antimicrobial activity.
      Authors: Keller MJ, Guzman E, Hazrati E, Kasowitz A, Cheshenko N, Wallenstein S, Cole AL, Cole AM, Profy AT, Wira CR, Hogarty K, Herold BC
      AIDS, 2007;21(4):467-76.
      Species: Human
      Sample Type: Vaginal Lavage
    34. Regulation of hepatocyte growth factor secretion by fibroblasts in patients with acute lung injury.
      Authors: Quesnel C, Marchand-Adam S, Fabre A, Marchal-Somme J, Philip I, Lasocki S, Lecon V, Crestani B, Dehoux M
      Am. J. Physiol. Lung Cell Mol. Physiol., 2007;294(2):L334-43.
      Species: Human
      Sample Type: BALF
    35. Decreased levels of peritoneal interleukin-1 receptor antagonist in patients with endometriosis and disease-related dysmenorrhea.
      Authors: Zhang X, Wen J, Deng L, Lin J
      Fertil. Steril., 2007;88(3):594-9.
      Species: Human
      Sample Type: Serum
    36. P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells.
      Authors: Wilson HL, Varcoe RW, Stokes L, Holland KL, Francis SE, Dower SK, Surprenant A, Crossman DC
      Br. J. Pharmacol., 2007;151(1):115-27.
      Species: Human
      Sample Type: Cell Culture Supernates
    37. Short communication: decreasing soluble CD30 and increasing IFN-gamma plasma levels are indicators of effective highly active antiretroviral therapy.
      Authors: Sadeghi M, Susal C, Daniel V, Naujokat C, Zimmermann R, Huth-Kuhne A, Opelz G
      AIDS Res. Hum. Retroviruses, 2007;23(7):886-90.
      Species: Human
      Sample Type: Plasma
    38. First in human phase I trial of 852A, a novel systemic toll-like receptor 7 agonist, to activate innate immune responses in patients with advanced cancer.
      Authors: Dudek AZ, Yunis C, Harrison LI, Kumar S, Hawkinson R, Cooley S, Vasilakos JP, Gorski KS, Miller JS
      Clin. Cancer Res., 2007;13(23):7119-25.
      Species: Human
      Sample Type: Serum
    39. Low concentration of interleukin-1beta induces FLICE-inhibitory protein-mediated beta-cell proliferation in human pancreatic islets.
      Authors: Maedler K, Schumann DM, Sauter N, Ellingsgaard H, Bosco D, Baertschiger R, Iwakura Y, Oberholzer J, Wollheim CB, Gauthier BR, Donath MY
      Diabetes, 2006;55(10):2713-22.
      Species: Human
      Sample Type: Cell Culture Supernates
    40. Strong inflammatory cytokine response in male and strong anti-inflammatory response in female kidney transplant recipients with urinary tract infection.
      Authors: Sadeghi M, Daniel V, Naujokat C, Wiesel M, Hergesell O, Opelz G
      Transpl. Int., 2005;18(2):177-85.
      Species: Human
      Sample Type: Plasma
    41. Circulating and synovial levels of IGF-I, cytokines, physical function and anthropometry differ in women awaiting total knee arthroplasty when compared to men.
      Authors: Pagura SM, Thomas SG, Woodhouse LJ, Ezzat S, Marks P
      J. Orthop. Res., 2005;23(2):397-405.
      Species: Human
      Sample Type: Synovial Fluid
    42. Experimental heatstroke in baboon: analysis of the systemic inflammatory response.
      Authors: Bouchama A, Ollivier V, Roberts G, Al Mohanna F, de Prost D, Eldali A, Saussereau E, El-Sayed R, Chollet-Martin S
      Shock, 2005;24(4):332-5.
      Species: Primate - Papio hamadryas (Hamadyras Baboon)
      Sample Type: Plasma
    43. Levels of soluble cytokine factors in temporomandibular joint effusions seen on magnetic resonance images.
      Authors: Kaneyama K, Segami N, Sun W, Sato J, Fujimura K
      Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2005;99(4):411-8.
      Species: Human
      Sample Type: Synovial Fluid
    44. Effects of the active metabolite of leflunomide, A77 1726, on cytokine release and the MAPK signalling pathway in human rheumatoid arthritis synoviocytes.
      Authors: Vergne-Salle&lt;/LastName&gt;&lt;F P&lt;/Initial, Vergne-Salle P, Leger DY, Bertin P, Treves R, Beneytout JL, Liagre B
      Cytokine, 2005;31(5):335-48.
      Species: Human
      Sample Type: Cell Culture Supernates
    45. Determinants of plasma leptin concentration in postmenopausal women.
      Authors: Brooks-Asplund EM, Tupper CE, Daun JM, Kenney WL, Cannon JG
      Mech. Ageing Dev., 2004;125(2):117-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    46. Effects of scaling and root planing on the amounts of interleukin-1 and interleukin-1 receptor antagonist and the mRNA expression of interleukin-1beta in gingival crevicular fluid and gingival tissues.
      Authors: Yoshinari N, Kawase H, Mitani A, Ito M, Sugiishi S, Matsuoka M, Shirozu N, Ishihara Y, Bito B, Hiraga M, Arakawa K, Noguchi T
      J. Periodont. Res., 2004;39(3):158-67.
      Species: Human
      Sample Type: Gingival Crevicular Fluid (GCF)
    47. IL-1 beta, IL-1 receptor antagonist and soluble type II IL-1 receptor in synovial fluid of patients with temporomandibular disorders.
      Authors: Tominaga K, Habu M, Sukedai M, Hirota Y, Takahashi T, Fukuda J
      Arch. Oral Biol., 2004;49(6):493-9.
      Species: Human
      Sample Type: Synovial Fluid
    48. CD14 is an acute-phase protein.
      Authors: Bas S, Gauthier BR, Spenato U, Stingelin S, Gabay C
      J. Immunol., 2004;172(7):4470-9.
      Species: Human
      Sample Type: Serum
    49. Guiding the vaginal microbicide trials with biomarkers of inflammation.
      Authors: Fichorova RN
      J. Acquir. Immune Defic. Syndr., 2004;37(0):S184-93.
      Species: Human
      Sample Type: Vaginal Lavage
    50. Impact of increased occlusal contact, interleukin-1 genotype, and periodontitis severity on gingival crevicular fluid IL-1beta levels.
      Authors: McDevitt MJ, Russell CM, Schmid MJ, Reinhardt RA
      J. Periodontol., 2003;74(9):1302-7.
      Species: Human
      Sample Type: Gingival Crevicular Fluid (GCF)
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