Human IL-2 QuantiGlo ELISA Kit

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  • Assay Length
    5.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
  • Sensitivity
    0.25 pg/mL
  • Assay Range
    1.7 - 1,250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human IL-2
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC191 , QuantiGlo Immunoassay Control Set 732 for Human IL-2 - Please Inquire
Product Summary
The QuantiGlo Human IL-2 Chemiluminescent Immunoassay is a 5.5 hour solid phase ELISA designed to measure IL-2 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-2 and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human IL-2. Results obtained using natural human IL-2 showed linear curves that were parallel to the standard curves obtained using the recombinant QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-2.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 5.8 175 884 5.9 165 795
Standard Deviation 0.19 5.6 23 0.46 11 59
CV% 3.3 3.2 2.6 7.8 6.7 7.4

Recovery

The recovery of IL-2 spiked to three different levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 102 99-105
EDTA Plasma (n=4) 97 95-100
Heparin Plasma (n=4) 97 94-104
Serum (n=4) 98 96-101
Linearity
To assess the linearity of the assay, four samples containing or spiked with high concentrations of IL-2 in various matrices were diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 IL-2 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-2
IL-2 (Interleukin 2) is a critical cytokine in T cell biology. It promotes T cell activation and expansion, the development, maintenance and function of regulatory T cells (Treg), and the differentiation of CD8+ T cells into terminal effector cells and memory cells. IL-2 signals through a receptor complex composed of CD25/IL-2 R alpha, IL-2 R beta, and the Common gamma Chain (gamma c). IL-2 R beta is also a component of the IL-15 receptor complex. Gamma c is also a signaling subunit in the receptors for IL-4, -7, -9, -15, and -21.
  • Long Name:
    Interleukin 2
  • Entrez Gene IDs:
    3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
  • Alternate Names:
    aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF
Related Research Areas

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QuantiGlo Immunoassay Control Set 732 for Human IL-2

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Average Rating: 5 (Based on 1 review)

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Sample Tested HEK293T human embryonic kidney cell line,HeLa human cervical epithelial carcinoma cell line,HOS human osteosarcoma cell line

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