Human IL-22 Quantikine ELISA Kit

  (23 citations)
(2 Reviews)
    
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Product Details
Assay Procedure
Citations (23)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Urine (100 uL)
  • Sensitivity
    5.8 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
  • Specificity
    Natural and recombinant human IL-22
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC29 , Quantikine Immunoassay Control Set 860 for Human IL-22 - Please Inquire
Product Summary
The Quantikine Human IL-22 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human IL-22 in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human IL-22 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-22 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring IL-22.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 56.5 227 468 63 243 491
Standard Deviation 2.55 7 10.7 5.32 12.1 19.6
CV% 4.5 3.1 2.3 8.4 5 4

Recovery

The recovery of human IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 107 104-110
EDTA Plasma (n=4) 97 90-103
Heparin Plasma (n=4) 95 86-104
Serum (n=4) 92 87-99
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IL-22 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 IL-22 [HRP]
Product Datasheets

Certificate of Analysis Lookup

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Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-22
IL-22 (Interleukin-22) is a cytokine that induces the production of reactive oxygen species, IL-6, IL-10, and TNF-alpha as well as neutrophil infiltration during inflammation. It also supports the integrity of epithelial barriers and induces epithelial cell proliferation during wound healing. IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta. IL-10 R beta is a shared component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29. IL-22 additionally binds to IL-22BP which blocks the interaction of IL-22 with IL-22 R.
  • Long Name:
    Interleukin 22
  • Entrez Gene IDs:
    50616 (Human); 50929 (Mouse); 500836 (Rat)
  • Alternate Names:
    Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL-21; IL22; IL-22; IL-22IL21; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 21; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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Species
Sample Type
  1. Diminished plasma levels of common ?-chain cytokines in pulmonary tuberculosis and reversal following treatment
    Authors: NP Kumar, VV Banurekha, D Nair, S Babu
    PLoS ONE, 2017;12(4):e0176495.
    Species: Human
    Sample Type: Plasma
  2. Lacrimal gland-derived IL-22 regulates IL-17-mediated ocular mucosal inflammation
    Authors: YW Ji, SK Mittal, HS Hwang, EJ Chang, JH Lee, Y Seo, A Yeo, H Noh, HS Lee, SK Chauhan, HK Lee
    Mucosal Immunol, 2017;0(0):.
    Species: Human
    Sample Type: Cell Culture Supernates
  3. Alterations in the Fecal Microbiota of Patients with HIV-1 Infection: An Observational Study in A Chinese Population
    Sci Rep, 2016;6(0):30673.
    Species: Human
    Sample Type: Serum
  4. Mycobacteria bypass mucosal NF-kB signalling to induce an epithelial anti-inflammatory IL-22 and IL-10 response.
    Authors: Lutay N, Hakansson G, Alaridah N, Hallgren O, Westergren-Thorsson G, Godaly G
    PLoS ONE, 2014;9(1):e86466.
    Species: Human
    Sample Type: Cell Culture Supernates
  5. Co-culture of human bronchial fibroblasts and CD4+ T cells increases Th17 cytokine signature.
    Authors: Loubaki L, Hadj-Salem I, Fakhfakh R, Jacques E, Plante S, Boisvert M, Aoudjit F, Chakir J
    PLoS ONE, 2013;8(12):e81983.
    Species: Human
    Sample Type: Cell Culture Supernates
  6. IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation.
    Authors: Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P
    PLoS Biol, 2012;10(9):e1001395.
    Species: Human
    Sample Type: Serum
  7. Interleukin-22 predicts severity and death in advanced liver cirrhosis: a prospective cohort study.
    Authors: Kronenberger B, Rudloff I, Bachmann M, Brunner F, Kapper L, Filmann N, Waidmann O, Herrmann E, Pfeilschifter J, Zeuzem S, Piiper A, Muhl H
    BMC Med, 2012;10(0):102.
    Species: Human
    Sample Type: Serum
  8. Preclinical Evaluation of Local JAK1 and JAK2 Inhibition in Cutaneous Inflammation.
    Authors: Fridman JS, Scherle PA, Collins R, Burn T, Neilan CL, Hertel D, Contel N, Haley P, Thomas B, Shi J, Collier P, Rodgers JD, Shepard S, Metcalf B, Hollis G, Newton RC, Yeleswaram S, Friedman SM, Vaddi K
    J. Invest. Dermatol., 2011;131(9):1838-44.
    Species: Human
    Sample Type: Cell Culture Supernates
  9. Complement component C5a Promotes Expression of IL-22 and IL-17 from Human T cells and its Implication in Age-related Macular Degeneration.
    Authors: Liu B, Wei L, Meyerle C, Tuo J, Sen HN, Li Z, Chakrabarty S, Agron E, Chan CC, Klein ML, Chew E, Ferris F, Nussenblatt RB
    J Transl Med, 2011;9(0):111.
    Species: Human
    Sample Type: Serum
  10. IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
    Authors: Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
    Clin. Cancer Res., 2011;17(24):7529-38.
    Species: Human
    Sample Type: Serum
  11. The IL23R R381Q Gene Variant Protects against Immune-Mediated Diseases by Impairing IL-23-Induced Th17 Effector Response in Humans.
    Authors: Di Meglio P, Di Cesare A, Laggner U
    PLoS ONE, 2011;6(2):e17160.
    Species: Human
    Sample Type: Cell Culture Supernates
  12. Kinetics of circulating Th17 cytokines and adipokines in psoriasis patients.
    Authors: Nakajima H, Nakajima K, Tarutani M, Morishige R, Sano S
    Arch. Dermatol. Res., 2011;303(6):451-5.
    Species: Human
    Sample Type: Serum
  13. Activation of the aryl hydrocarbon receptor reveals distinct requirements for IL-22 and IL-17 production by human T helper cells.
    Authors: Ramirez JM, Brembilla NC, Sorg O, Chicheportiche R, Matthes T, Dayer JM, Saurat JH, Roosnek E, Chizzolini C
    Eur. J. Immunol., 2010;40(9):2450-9.
    Species: Human
    Sample Type: Cell Culture Supernates
  14. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
    Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Allergy Clin. Immunol., 2009;123(1):59-66.e4.
    Species: Human
    Sample Type: Cell Culture Supernates
  15. Prostaglandin E2 regulates Th17 cell differentiation and function through cyclic AMP and EP2/EP4 receptor signaling.
    Authors: Boniface K, Bak-Jensen KS, Li Y, Blumenschein WM, McGeachy MJ, McClanahan TK, McKenzie BS, Kastelein RA, Cua DJ, de Waal Malefyt R
    J. Exp. Med., 2009;206(3):535-48.
    Species: Human
    Sample Type: Cell Culture Supernates
  16. The Th17 cytokine IL-22 induces IL-20 production in keratinocytes: a novel immunological cascade with potential relevance in psoriasis.
    Authors: Wolk K, Witte E, Warszawska K, Schulze-Tanzil G, Witte K, Philipp S, Kunz S, Docke WD, Asadullah K, Volk HD, Sterry W, Sabat R
    Eur. J. Immunol., 2009;39(12):3570-81.
    Species: Human
    Sample Type: Serum
  17. Dexamethasone suppresses interleukin-22 associated with bacterial infection in vitro and in vivo.
    Authors: Ziesche E, Scheiermann P, Bachmann M, Sadik CD, Hofstetter C, Zwissler B, Pfeilschifter J, Muhl H
    Clin. Exp. Immunol., 2009;157(3):370-6.
    Species: Human
    Sample Type: Cell Culture Supernates
  18. The role of interleukin-22 in hepatitis C virus infection.
    Authors: Dambacher J, Beigel F, Zitzmann K, Heeg MH, Goke B, Diepolder HM, Auernhammer CJ, Brand S
    Cytokine, 2008;41(3):209-16.
    Species: Human
    Sample Type: Serum
  19. Chronically inflamed human tissues are infiltrated by highly differentiated Th17 lymphocytes.
    Authors: Pene J, Chevalier S, Preisser L, Venereau E, Guilleux MH, Ghannam S, Moles JP, Danger Y, Ravon E, Lesaux S, Yssel H, Gascan H
    J. Immunol., 2008;180(0):7423.
    Species: Human
    Sample Type: Cell Culture Supernates
  20. IL-22 mediates mucosal host defense against Gram-negative bacterial pneumonia.
    Authors: Aujla SJ, Chan YR, Zheng M, Fei M, Askew DJ, Pociask DA, Reinhart TA, McAllister F, Edeal J, Gaus K, Husain S, Kreindler JL, Dubin PJ, Pilewski JM, Myerburg MM, Mason CA, Iwakura Y, Kolls JK
    Nat. Med., 2008;14(3):275-81.
    Species: Human
    Sample Type: Cell Culture Supernates
  21. Linking genetic susceptibility to Crohn's disease with Th17 cell function: IL-22 serum levels are increased in Crohn's disease and correlate with disease activity and IL23R genotype status.
    Authors: Schmechel S, Konrad A, Diegelmann J, Glas J, Wetzke M, Paschos E, Lohse P, Goke B, Brand S
    Inflamm. Bowel Dis., 2008;14(2):204-12.
    Species: Human
    Sample Type: Serum
  22. IL-22 induces lipopolysaccharide-binding protein in hepatocytes: a potential systemic role of IL-22 in Crohn's disease.
    Authors: Wolk K, Witte E, Hoffmann U, Doecke WD, Endesfelder S, Asadullah K, Sterry W, Volk HD, Wittig BM, Sabat R
    J. Immunol., 2007;178(9):5973-81.
    Species: Human
    Sample Type: Cell Culture Supernates
  23. IL-22 regulates the expression of genes responsible for antimicrobial defense, cellular differentiation, and mobility in keratinocytes: a potential role in psoriasis.
    Authors: Wolk K, Witte E, Wallace E, Docke WD, Kunz S, Asadullah K, Volk HD, Sterry W, Sabat R
    Eur. J. Immunol., 2006;36(5):1309-23.
    Species: Human
    Sample Type: Plasma
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Quantikine Immunoassay Control Set 860 for Human IL-22

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Average Rating: 5 (Based on 2 reviews)

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  Anonymous 11/30/2017
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Sample Tested B16-F10 mouse melanoma cell line
 Human IL-22 Quantikine ELISA Kit D2200 Array D2200
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  Anonymous 09/07/2017
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 Human IL-22 Quantikine ELISA Kit D2200
: Human IL-22 Quantikine ELISA Kit [D2200].

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Sample Tested Serum

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Other Experimental Details The IL-22 Quantikine ELISA performed as expected with low % CV's, and reproducible results.

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