Human IL-3 Quantikine ELISA Kit

R&D Systems | Catalog # D3000

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (200 µL), Serum (200 µL), EDTA Plasma (200 µL), Heparin Plasma (200 µL), Citrate Plasma (200 µL)

Sensitivity

7.4 pg/mL

Assay Range

31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
View all IL-3 ELISA Kits »

Product Summary for Human IL-3 Quantikine ELISA Kit

The Quantikine Human IL-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-3 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-3 and antibodies raised against the recombinant factor. It has been shown to quantitate the recombinant factor accurately.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human IL-3

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 126 731 991 128 725 1423
Standard Deviation 5.7 23.7 30.4 10.1 45.9 81.5
CV% 4.5 3.2 3.1 7.9 6.3 5.7

Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 115 728 1460 124 737 1450
Standard Deviation 6.5 23.6 48.7 13.3 41.4 104
CV% 5.7 3.2 3.3 10.7 5.6 7.2

Recovery for Human IL-3 Quantikine ELISA Kit

The recovery of human IL-3 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 92 78-105
Citrate Plasma (n=0) 104 93-122
EDTA Plasma (n=0) 108 96-117
Heparin Plasma (n=0) 100 93-108
Serum (n=5) 104 96-118

Linearity

To assess the linearity of the assay, the following samples spiked with high concentrations of IL-3 were diluted with the appropriate Calibrator Diluent and then assayed.

Human IL-3 ELISA Linearity

Scientific Data Images for Human IL-3 Quantikine ELISA Kit

Human IL-3 ELISA Cell Culture Supernate Standard Curve

Human IL-3 ELISA Cell Culture Supernate Standard Curve

Human IL-3 ELISA Serum/Plasma Standard Curve

Human IL-3 ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-3

Interleukin-3 (IL-3) is a secreted cytokine that stimulates the proliferation and differentiation of pluripotent hematopoietic stem cells and various lineage-committed progenitors. It also regulates the activation of mature mast cells, basophils, eosinophils, and macrophages. It is produced by activated T cells, thymic epithelial cells, activated mast cells, keratinocytes, neurons, and astrocytes. IL-3 signals through a receptor complex that contains IL-3 R alpha/CD123 and the common beta chain which is also a component of the receptors for IL-5 and GM-CSF. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils, and various myeloid leukemic cells. Human IL-3 does not show activity on mouse cells.

Long Name

Interleukin 3

Alternate Names

IL3, MCGF, MULTI-CSF

Entrez Gene IDs

3562 (Human); 16187 (Mouse); 24495 (Rat)

Gene Symbol

IL3

Additional IL-3 Products

Product Documents for Human IL-3 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IL-3 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human IL-3 Quantikine ELISA Kit

Customer Reviews for Human IL-3 Quantikine ELISA Kit (3)

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  • Human IL-3 Quantikine ELISA Kit
    Name: Leslie Priddy
    Sample Tested: Serum
    Verified Customer | Posted 04/01/2018
  • Human IL-3 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: CHO Chinese hamster ovary cell line
    Verified Customer | Posted 12/07/2017
  • Human IL-3 Quantikine ELISA Kit
    Name: Prakasha Kempaiah
    Sample Tested: Serum
    Verified Customer | Posted 06/03/2016

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Protocols

View specific protocols for Human IL-3 Quantikine ELISA Kit (D3000):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent for Serum & Plasma Samples Only
  3.   For Serum & Plasma Samples Only: Add 50 µL of Assay Diluent to each well.
    4. 200 µL Standard, Control, or Sample
  4.   Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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FAQs

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