Human IL-3 Quantikine ELISA Kit

  (15 citations)
(3 Reviews)
    
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Product Details
Assay Procedure
Citations (15)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL)
  • Sensitivity
    7.4 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human IL-3
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC21 , Quantikine Immunoassay Control Group 4 - Please Inquire
Product Summary
The Quantikine Human IL-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-3 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-3 and antibodies raised against the recombinant factor. It has been shown to quantitate the recombinant factor accurately.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 126 731 991 128 725 1423
Standard Deviation 5.7 23.7 30.4 10.1 45.9 81.5
CV% 4.5 3.2 3.1 7.9 6.3 5.7

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 115 728 1460 124 737 1450
Standard Deviation 6.5 23.6 48.7 13.3 41.4 104
CV% 5.7 3.2 3.3 10.7 5.6 7.2

Recovery

The recovery of human IL-3 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 92 78-105
Citrate Plasma (n=) 104 93-122
EDTA Plasma (n=) 108 96-117
Heparin Plasma (n=) 100 93-108
Serum (n=5) 104 96-118
Linearity
To assess the linearity of the assay, the following samples spiked with high concentrations of IL-3 were diluted with the appropriate Calibrator Diluent and then assayed.
 IL-3 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-3
Interleukin-3 (IL-3) is a secreted cytokine that stimulates the proliferation and differentiation of pluripotent hematopoietic stem cells and various lineage-committed progenitors. It also regulates the activation of mature mast cells, basophils, eosinophils, and macrophages. It is produced by activated T cells, thymic epithelial cells, activated mast cells, keratinocytes, neurons, and astrocytes. IL-3 signals through a receptor complex that contains IL-3 R alpha/CD123 and the common beta chain which is also a component of the receptors for IL-5 and GM-CSF. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils, and various myeloid leukemic cells. Human IL-3 does not show activity on mouse cells.
  • Long Name:
    Interleukin 3
  • Entrez Gene IDs:
    3562 (Human); 16187 (Mouse); 24495 (Rat)
  • Alternate Names:
    Hematopoietic growth factor; IL3; IL-3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent for Serum & Plasma Samples Only
  4.   For Serum & Plasma Samples Only: Add 50 µL of Assay Diluent to each well.

  5. 200 µL Standard, Control, or Sample
  6.   Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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Species
Sample Type
  1. Hematological parameters, and hematopoietic growth factors: EPO and IL-3 in response to whole-body cryostimulation (WBC) in military academy students.
    Authors: Szygula Z, Lubkowska A, Giemza C, Skrzek A, Bryczkowska I, Dolegowska B
    PLoS ONE, 2014;9(4):e93096.
    Species: Human
    Sample Type: Plasma
  2. The systemic cytokine environment is permanently altered in multiple myeloma.
    Authors: Zheng M, Zhang Z, Bemis K, Belch A, Pilarski L, Shively J, Kirshner J
    PLoS ONE, 2013;8(3):e58504.
    Species: Human
    Sample Type: Plasma
  3. Chronic immune activation in HIV-1 infection contributes to reduced interferon alpha production via enhanced CD40:CD40 ligand interaction.
    Authors: Donhauser N, Pritschet K, Helm M, Harrer T, Schuster P, Ries M, Bischof G, Vollmer J, Smola S, Schmidt B
    PLoS ONE, 2012;7(3):e33925.
    Species: Human
    Sample Type: Plasma
  4. Interleukin-3 is elevated in patients with coronary artery disease and predicts restenosis after percutaneous coronary intervention.
    Authors: Rudolph T, Schaps KP, Steven D, Koester R, Rudolph V, Berger J, Terres W, Meinertz T, Kaehler J
    Int. J. Cardiol., 2009;132(3):392-7.
    Species: Human
    Sample Type: Serum
  5. Functional interaction of plasmacytoid dendritic cells with multiple myeloma cells: a therapeutic target.
    Authors: Chauhan D, Singh AV, Brahmandam M, Carrasco R, Bandi M, Hideshima T, Bianchi G, Podar K, Tai YT, Mitsiades C, Raje N, Jaye DL, Kumar SK, Richardson P, Munshi N, Anderson KC
    Cancer Cell, 2009;16(4):309-23.
    Species: Human
    Sample Type: Cell Culture Supernates
  6. HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells.
    Authors: Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A
    J. Exp. Med., 2009;206(1):169-81.
    Species: Human
    Sample Type: Cell Culture Supernates
  7. Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
    Authors: Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G
    Transplantation, 2009;88(9):1109-16.
    Species: Human
    Sample Type: Urine
  8. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008;36(8):1035-46.
    Species: Human
    Sample Type: Cell Culture Supernates
  9. Short communication: decreasing soluble CD30 and increasing IFN-gamma plasma levels are indicators of effective highly active antiretroviral therapy.
    Authors: Sadeghi M, Susal C, Daniel V, Naujokat C, Zimmermann R, Huth-Kuhne A, Opelz G
    AIDS Res. Hum. Retroviruses, 2007;23(7):886-90.
    Species: Human
    Sample Type: Plasma
  10. cIAP-2 and survivin contribute to cytokine-mediated delayed eosinophil apoptosis.
    Authors: Vassina EM, Yousefi S, Simon D, Zwicky C, Conus S, Simon HU
    Eur. J. Immunol., 2006;36(7):1975-84.
    Species: Human
    Sample Type: Serum
  11. Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts.
    Authors: Hatfield K, Ryningen A, Corbascio M, Bruserud O
    Int. J. Cancer, 2006;119(10):2313-21.
    Species: Human
    Sample Type: Cell Culture Supernates
  12. Strong inflammatory cytokine response in male and strong anti-inflammatory response in female kidney transplant recipients with urinary tract infection.
    Authors: Sadeghi M, Daniel V, Naujokat C, Wiesel M, Hergesell O, Opelz G
    Transpl. Int., 2005;18(2):177-85.
    Species: Human
    Sample Type: Plasma
  13. Single administration of stem cell factor, FLT-3 ligand, megakaryocyte growth and development factor, and interleukin-3 in combination soon after irradiation prevents nonhuman primates from myelosuppression: long-term follow-up of hematopoiesis.
    Authors: Drouet M, Mourcin F, Grenier N, Leroux V, Denis J, Mayol JF, Thullier P, Lataillade JJ, Herodin F
    Blood, 2004;103(3):878-85.
  14. Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte macrophage-colony-stimulating factor.
    Authors: Bovolenta C, Pilotti E, Mauri M, Turci M, Ciancianaini P, Fisicaro P, Bertazzoni U, Poli G, Casoli C
    Blood, 2002;99(1):224-31.
    Species: Human
    Sample Type: Cell Culture Supernates
  15. Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
    Authors: Kadowaki N, Antonenko S, Lau JY, Liu YJ
    J. Exp. Med., 2000;192(2):219-26.
    Species: Human
    Sample Type: Cell Culture Supernates
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