Human IL‑3R alpha /CD123 Biotinylated Antibody

Name: Human IL‑3R alpha /CD123 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF841
Price: 489 USD
Availability: InStock

R&D Systems | Catalog # BAF841

Submit a review

Product Documents

View Available Conjugates

100% Guarantee

Product Specifications
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all IL-3R alpha Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑3 R alpha /CD123
Extracellular domain

Specificity

Detects human IL‑3 R alpha /CD123 in Western blots. In Western blots, less than 5% cross‑reactivity with recombinant human (rh) IL-1 sRI, rhIL‑1 sRII, rhIL-2 sR beta, rhIL-2 sR gamma, rhIL-5 sR alpha, and rhIL-10 sR is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human IL‑3R alpha /CD123 Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human IL‑3 R alpha /CD123 (Catalog # 301-R3)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-3R alpha

Interleukin 3 is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity human IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta subunit does not bind IL-3 by itself but is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF. Both the alpha and the beta subunits are members of the cytokine receptor superfamily.