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Human IL-4 Antibody (34019)

R&D Systems | Catalog # MAB204

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Zebrafish

Applications

Validated:

Western Blot, Neutralization

Cited:

Western Blot, Neutralization, Flow Cytometry, Bioassay, Blocking, Differentiation, In vivo assay, Functional Assay, Stimulation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 34019
View all IL-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL‑4
His25-Ser153
Accession # P05112

Specificity

Detects human IL-4 in Western blots. In Western blots, no cross-reactivity with recombinant mouse IL‑4, recombinant porcine IL‑4, recombinant cotton rat IL‑4, recombinant rat IL‑4, or recombinant human IL‑13 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL-4 Antibody (34019)

Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 Antibody.

Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 Antibody.

Recombinant Human IL-4 (Catalog # 204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-4 Monoclonal Antibody (Catalog # MAB204). The ND50 is typically 0.5-1.5 µg/mL.
Detection of IL-4 by Western Blot

Detection of IL-4 by Western Blot

IL-12 does not inhibit 1,25(OH)2D3 production or VDR expression and function in CD4+ T cells. Relative CYP27B1 (A), VDR (C), and CYP24A1 (E) expression in T cells activated for 72 h in the presence of 100 nM 25(OH)D3 and the indicated concentration of IL-12. Data are normalized to activated T cells incubated with 100 nM 25(OH)D3 in the absence of IL-12 (mean + SEM, n ≥ 6). (B) 1,25(OH)2D3 production in T cells activated for 72 h in the presence of 100 nM 25(OH)D3 and the indicated concentration of IL-12 (mean + SEM, n ≥ 4). (D) Representative Western blot (lower panel) and quantification (upper panel) of VDR with GAPDH as loading control from T cells activated for 72 h in the presence of 100 nM 25(OH)D3 and in the presence or absence of 10 ng/ml IL-12 (mean + SEM, n = 4). Western blots including protein ladder are shown in the Figure S1 in Supplementary Material. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28620394), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human IL-4 Antibody (34019)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human IL‑4 (Catalog # 204-IL)

Neutralization

Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.5-1.5 µg/mL in the presence of 0.5 ng/mL Recombinant Human IL‑4.

Reviewed Applications

Read 2 reviews rated 4 using MAB204 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39%, and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5-7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma  chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on non-hematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10-13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

References

  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894.
  4. Redfield, C. et al. (1991) Biochemistry 30:11029.
  5. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
  6. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
  7. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
  8. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  9. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  10. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  11. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  12. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  13. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  14. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

Long Name

Interleukin 4

Alternate Names

BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL4, IL4E12

Entrez Gene IDs

3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 751514 (Feline); 574281 (Primate); 100302454 (Rabbit)

Gene Symbol

IL4

UniProt

P05112

Additional IL-4 Products

Product Documents for Human IL-4 Antibody (34019)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL-4 Antibody (34019)

For research use only

Citations for Human IL-4 Antibody (34019)

Customer Reviews for Human IL-4 Antibody (34019) (2)

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2 Customer Ratings
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  • Human IL-4 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/06/2019
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human IL-4 Antibody (34019) MAB204
  • Human IL-4 Antibody
    Name: Priyank raje
    Application: ELISA
    Sample Tested: T cells
    Species: Human and Rat
    Verified Customer | Posted 12/03/2017

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