hh Human IL-4 Antibody MAB204-100: R&D Systems

Human IL-4 Antibody

(8 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-4 in Western blots. In Western blots, no cross-reactivity with recombinant mouse IL‑4, recombinant porcine IL‑4, recombinant cotton rat IL‑4, recombinant rat IL‑4, or recombinant human IL‑13 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 34019
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human IL‑4
    His25-Ser153
    Accession # P05112
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human IL‑4 (Catalog # 204-IL)
  • Neutralization
    Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.5-1.5 µg/mL in the presence of 0.5 ng/mL Recombinant Human IL‑4.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 Antibody. Recombinant Human IL‑4 (Catalog # 204-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑4 Monoclonal Antibody (Catalog # MAB204). The ND50 is typically 0.5-1.5 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39%, and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5-7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma  chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on non-hematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10-13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

  • References:
    1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
    2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
    3. Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894.
    4. Redfield, C. et al. (1991) Biochemistry 30:11029.
    5. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
    6. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
    7. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
    8. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
    9. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
    10. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
    11. Corthay, A. (2006) Scand. J. Immunol. 64:93.
    12. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
    13. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
    14. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
  • Long Name:
    Interleukin 4
  • Entrez Gene IDs:
    3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
  • Alternate Names:
    B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Vitamin D up-regulates the vitamin D receptor by protecting it from proteasomal degradation in human CD4+ T cells.
    Authors: Kongsbak M, von Essen M, Boding L, Levring T, Schjerling P, Lauritsen J, Woetmann A, Odum N, Bonefeld C, Geisler C
    PLoS ONE, 2014;9(5):e96695.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Impaired development of human Th1 cells in patients with deficient expression of STAT4.
    Authors: Chang HC, Han L, Goswami R, Nguyen ET, Pelloso D, Robertson MJ, Kaplan MH
    Blood, 2009;113(23):5887-90.
    Species: Human
    Sample Type: Whole Cells
    Application: Other: Cell differentiation
  3. Normally occurring NKG2D+CD4+ T cells are immunosuppressive and inversely correlated with disease activity in juvenile-onset lupus.
    Authors: Dai Z, Turtle CJ, Booth GC, Riddell SR, Gooley TA, Stevens AM, Spies T, Groh V
    J. Exp. Med., 2009;206(4):793-805.
    Species: Human
    Sample Type: Whole Cells
    Application: Blocking
  4. Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells.
    Authors: Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F
    Nat. Immunol., 2007;8(9):942-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. Cytokine milieu of atopic dermatitis skin subverts the innate immune response to vaccinia virus.
    Authors: Howell MD, Gallo RL, Boguniewicz M, Jones JF, Wong C, Streib JE, Leung DY
    Immunity, 2006;24(3):341-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006;120(2):220-31.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  7. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006;241(1):7-13.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  8. Cutting edge: immune cells as sources and targets of the IL-10 family members?
    Authors: Wolk K, Kunz S, Asadullah K, Sabat R
    J. Immunol., 2002;168(11):5397-402.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
Isotype Controls
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

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Mouse IgG2B Antibody

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Rat Anti-Mouse IgG2B PE-conjugated Antibody

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Average Rating: 5 (Based on 1 review)

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ApplicationELISA
Sample TestedT cells

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