Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Zebrafish

Applications

Validated:

Western Blot, Neutralization

Cited:

Western Blot, Neutralization, Flow Cytometry, Bioassay, Blocking, Differentiation, In vivo assay, Functional Assay, Stimulation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 34019
View all IL-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL‑4
His25-Ser153
Accession # P05112

Specificity

Detects human IL-4 in Western blots. In Western blots, no cross-reactivity with recombinant mouse IL‑4, recombinant porcine IL‑4, recombinant cotton rat IL‑4, recombinant rat IL‑4, or recombinant human IL‑13 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL‑4 Antibody

Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 Antibody.

Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 Antibody.

Recombinant Human IL-4 (Catalog # 204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-4 Monoclonal Antibody (Catalog # MAB204). The ND50 is typically 0.5-1.5 µg/mL.
Detection of IL-4 by Western Blot

Detection of IL-4 by Western Blot

IL-12 does not inhibit 1,25(OH)2D3 production or VDR expression and function in CD4+ T cells. Relative CYP27B1 (A), VDR (C), and CYP24A1 (E) expression in T cells activated for 72 h in the presence of 100 nM 25(OH)D3 and the indicated concentration of IL-12. Data are normalized to activated T cells incubated with 100 nM 25(OH)D3 in the absence of IL-12 (mean + SEM, n ≥ 6). (B) 1,25(OH)2D3 production in T cells activated for 72 h in the presence of 100 nM 25(OH)D3 and the indicated concentration of IL-12 (mean + SEM, n ≥ 4). (D) Representative Western blot (lower panel) and quantification (upper panel) of VDR with GAPDH as loading control from T cells activated for 72 h in the presence of 100 nM 25(OH)D3 and in the presence or absence of 10 ng/ml IL-12 (mean + SEM, n = 4). Western blots including protein ladder are shown in the Figure S1 in Supplementary Material. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28620394), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human IL‑4 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human IL‑4 (Catalog # 204-IL)

Neutralization

Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.5-1.5 µg/mL in the presence of 0.5 ng/mL Recombinant Human IL‑4.

Reviewed Applications

Read 2 reviews rated 4 using MAB204 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39%, and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5-7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma  chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on non-hematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10-13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

References

  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894.
  4. Redfield, C. et al. (1991) Biochemistry 30:11029.
  5. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
  6. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
  7. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
  8. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  9. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  10. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  11. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  12. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  13. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  14. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

Long Name

Interleukin 4

Alternate Names

BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL4, IL4E12

Entrez Gene IDs

3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 751514 (Feline); 574281 (Primate); 100302454 (Rabbit)

Gene Symbol

IL4

UniProt

P05112

Additional IL-4 Products

Product Documents for Human IL‑4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL‑4 Antibody

For research use only

Citations for Human IL‑4 Antibody

Customer Reviews for Human IL‑4 Antibody (2)

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2 Customer Ratings
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  • Human IL-4 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/06/2019
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human IL‑4 Antibody MAB204
  • Human IL-4 Antibody
    Name: Priyank raje
    Application: ELISA
    Sample Tested: T cells
    Species: Human and Rat
    Verified Customer | Posted 12/03/2017

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