Human IL-4 Magnetic Luminex® Performance Assay

Name: Human IL-4 Magnetic Luminex® Performance Assay
Brand: R&D Systems
SKU: LUHM204
Price: 210 USD
Availability: InStock

R&D Systems | Catalog # LUHM204

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Product Documents

Luminex High Performance Assays

Human IL-4 Magnetic Luminex® Performance Assay is specifically designed for multiplex detection of human IL-4 with a panel of related analytes in serum, plasma, or tissue culture supernatants. Luminex High Performance Assays go through rigorous development, validation, and quality control testing to ensure the best performance of your multiplex assay.

This assay is customizable, select some or all the analytes in the entire panel. Customize your panel with the Luminex Assay Customization Tool.

Build Now

Build Your Assay
Product Features
Performance Validation
Kit Contents
Precision and Accuracy
Which Luminex Assay is Right for Me
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Panel

Human Cytokine Panel A

Assay Type

Magnetic bead-based multiplex assay for the Luminex® platform

Format

1 x 96-well microplate and magnetic antibody-coated beads

Species

Human

Analytes Detected

IL-4

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.

View all IL-4 Luminex Assays »

Build Your Assay

Make Selections Below to Configure Your Custom Luminex® Assay

Configured Assays

Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.

If overlapping bead regions occur in analyte selections, send your Luminex configuration code to our team for resolution.

How would you like these analytes mixed?

Premixed

User Mixed

Please select at least one mixing option to see the kits.

Would you like to split based on sample dilution?

Do not split

Split based on serum

Split based on plasma

Split based on cell culture supernates

Product Features

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

Simultaneously profile Human IL-4 with a panel of related analytes in one sample
Magnetic format allows for easier wash steps
Undergoes similar validation testing as Quantikine® ELISA Kits
Precise and Specific
Requires a small sample volume (<50 µL)
Run your assay in just 3-5 hours
Mass-calibrated standards for consistent results with every new lot of material
Enough reagents for one 96-well plate
Quick turn-around time: Kits are typically shipped within 2-3 days of ordering*

*Please allow additional time for international orders.

Performance Validation

R&D Systems Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

Base Kit Contents

Standard Cocktail(s)
Bead Diluent
Biotin Antibody Diluent
Calibrator Diluent
Wash Buffer
Streptavidin-PE
One flat-bottom 96-well Microplate
Foil Plate Sealers (4)
Mixing Bottle
Standard Value Card**

**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

Bead Set Contents

User-mixed antibody-coated Magnetic beads in individual vials
User-mixed cocktail of biotinylated detection antibody

Precision

	Intra-Assay Precision			Inter-Assay Precision
Intra-assay Precision (precision within an assay) - Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (precision between assays) - Three samples of known concentration were tested in twenty separate assays to assess precision between assays.
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	23	57	369	65	430	835
Standard deviation	0.98	1.9	11.2	7.1	68.3	78.9
CV (%)	4.3	3.3	3.0	10.9	15.9	9.4

Recovery

Sample Type	Average % Recovery	Range (%)
Samples containing and/or spiked with human IL-4 were evaluated for recovery.
Cell culture supernate	81	69-93
Serum	103	77-126
EDTA plasma	106	84-123
Heparin plasma	104	94-114

Linearity

		Cell culture supernate	Serum	EDTA plasma	Heparin plasma
Samples containing and/or spiked with human IL-4 were serially diluted to evaluate assay linearity.
1:2	Average % of Expected	84	105	98	95
1:2	Range (%)	74-97	93-119	93-106	90-102
1:4	Average % of Expected	86	106	94	91
1:4	Range (%)	74-98	94-121	90-101	85-100
1:8	Average % of Expected	94	103	90	96
1:8	Range (%)	83-108	93-120	76-100	87-107

Which Luminex Assay is Right for Me?

Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays.

High Performance Assays are optimized and validated as complete panels with a focused set of analytes. You can choose from Fixed Panels, Premixed options, or Configurable User Mix formats depending on your needs.

Discovery Assays deliver maximum flexibility, enabling the creation of fully customized panels tailored to your research needs. With universal reagents validated for compatibility across all analytes in the menu, they support endless customization possibilities.

Additional Information About Luminex Assays

All R&D Systems Luminex Assays are designed for use with any Luminex instrument including MAGPIX, Luminex 200, FLEXMAP 3D, and the INTELLIFLEX.

Luminex assays use color-coded, magnetic microparticles coated with analyte-specific antibodies to enable multiplex quantitation. During the assay, standards and samples are added to the wells, where target analytes bind to the capture antibodies. A biotinylated detection antibody cocktail is then introduced, followed by a Streptavidin-PE conjugate that generates a fluorescent signal proportional to the amount of analyte present.

Each microparticle is identified by a unique signature and the signal intensity is measured using a Luminex instrument. These assays are designed to deliver reliable and reproducible results with validated antibody pairs and optimized protocols. They are well suited for applications that require sensitivity, accuracy, and confidence in your data while enabling efficient multiplexing to measure multiple targets simultaneously.

Scientific Data Images for Human IL-4 Magnetic Luminex® Performance Assay

Quantist software graph of signal intensity versus concentration for IL-4 in the Human Cytokine A Luminex Panel

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-4

IL-4 (Interleukin-4) is a cytokine that is primarily expressed by Th2-biased CD4⁺ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naÃ¯ve CD4⁺ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. The type I receptor for IL-4, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals.