Human IL-4 QuantiGlo ELISA Kit

Name: Human IL-4 QuantiGlo ELISA Kit
Brand: R&D Systems
SKU: Q4000

R&D Systems | Catalog # Q4000

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Product Documents

Discontinued Product

Q4000 has been discontinued. View all IL-4 products.

Product Summary
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

5.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (150 µL), Serum (150 µL), EDTA Plasma (150 µL), Heparin Plasma (150 µL), Citrate Plasma (150 µL)

Sensitivity

1 pg/mL

Assay Range

1.6-5000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)

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Product Summary for Human IL-4 QuantiGlo ELISA Kit

The QuantiGlo Human IL-4 Chemiluminescent Immunoassay is a 5.5 hour solid phase ELISA designed to measure human IL-4 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-4 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-4. Results obtained using natural human IL-4 showed dose response curves that were parallel to the standard curves obtained using the recombinant QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-4.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Chemiluminescent - Luminometer (Luminol)

Species

Human

Specificity

Natural and recombinant human IL-4

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Four samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Four samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates

	Intra-Assay Precision				Inter-Assay Precision
Sample	1	2	3	4	1	2	3	4
n	20	20	20	20	40	40	40	40
Mean (pg/mL)	2.38	10.1	125	1306	2.80	11.9	137	1400
Standard Deviation	0.21	0.85	8.13	100	0.41	1.0	7.2	60
CV%	8.8	8.4	6.5	7.7	14.6	8.4	5.3	4.3

Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision				Inter-Assay Precision
Sample	1	2	3	4	1	2	3	4
n	20	20	20	20	40	40	40	40
Mean (pg/mL)	3.63	18.9	177	1666	3.7	15.9	166	1547
Standard Deviation	0.34	0.56	8.39	78.1	0.43	1.3	13.0	117
CV%	9.4	3.0	4.7	4.7	11.6	8.2	7.8	7.6

Recovery for Human IL-4 QuantiGlo ELISA Kit

The recovery of human IL-4 spiked to three different levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=9)	103	87-115
Citrate Plasma (n=6)	91	83-100
EDTA Plasma (n=6)	92	78-106
Heparin Plasma (n=6)	92	82-102
Serum (n=6)	93	76-109

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of IL-4 in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line

Scientific Data Images for Human IL-4 QuantiGlo ELISA Kit

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IL-4

IL-4 (Interleukin-4) is a cytokine that is primarily expressed by Th2-biased CD4⁺ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naÃ¯ve CD4⁺ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. The type I receptor for IL-4, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals.