Detects human IL‑4 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-9 R, recombinant mouse IL-4 R, rhIL-5 R alpha, rhIL-5 R beta, rhIL-13 R alpha 1, or rhIL-13 R alpha 2 is observed.
Monoclonal Mouse IgG2A Clone # 25463
Protein A or G purified from ascites
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑4 R Gly24-His232 Accession # P24394
Supplied in a saline solution containing BSA and Sodium Azide.
PerCP (Peridinin-chlorophyll Protein Complex)
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of IL‑4 R alpha in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IL‑4 R alpha PerCP‑conjugated Monoclonal Antibody (Catalog # FAB230C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-4 R alpha
Interleukin 4 receptor (IL-4 R) is the transmembrane ligand binding subunit of the heterodimeric IL-4 receptor complex. In the type I receptor complex, IL-4 R associates with the common gamma chain, whereas in the type II receptor complex, it associates with IL-13 R alpha 1. IL-4 R is expressed by B cells, T cells, monocytes and macrophages.
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