Insulin-like 6 (INSL6) is a member of the insulin family that is expressed in the testis by germ cells in the seminiferous tubules. Intracellular INSL6 is processed to a complex of two disulfide-linked peptides for secretion. Human and mouse INSL6 share 43% aa identity in the 213 aa, 28 kDa unprocessed form and 53% aa identity in the 64 aa, 8 kDa processed form.
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 421401
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Product Specifications
Immunogen
E. coli-derived recombinant human INSL6
Arg21-Tyr213
Accession # Q9Y581
Arg21-Tyr213
Accession # Q9Y581
Specificity
Detects human INSL6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) INSL3 and rhINSL4 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Human INSL6 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human INSL6
Sample: Recombinant Human INSL6
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: INSL6
Long Name
Insulin-like 6
Alternate Names
RIF1
Gene Symbol
INSL6
UniProt
Additional INSL6 Products
Product Documents for Human INSL6 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human INSL6 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: Western Blot Figures
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- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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