Human Integrin  alpha 4 beta 7/LPAM-1 (Research Grade Vedolizumab Biosimilar) Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibody For Research Use Only
Catalog # Availability Size / Price Qty
FAB10078R-100UG
Detection of Integrin alpha 4 beta 7/LPAM-1 in Human PBMC by Flow Cytometry.
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Human Integrin  alpha 4 beta 7/LPAM-1 (Research Grade Vedolizumab Biosimilar) Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha 4 beta 7/LPAM‑1 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Vedolizumab. This product is for research use only.
Source
Recombinant Monoclonal Human IgG1 Clone # Hu117
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human Integrin alpha 4 beta 7/LPAM‑1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Human PBMC

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Integrin alpha 4 beta 7/LPAM-1 antibody in Human PBMC antibody by Flow Cytometry. View Larger

Detection of Integrin alpha 4 beta 7/LPAM-1 in Human PBMC by Flow Cytometry. Human PBMC were stained with (A) Human Anti-Human Integrin alpha 4 beta 7/LPAM-1 (Research Grade Vedolizumab Biosimilar) Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB10078R) or (B) no primary antibody, followed by Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (FAB100P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 4 beta 7/LPAM-1

Vedolizumab is a biosimilar that binds and blocks binds to integrin alpha 4 beta 7. This product can be used in flow cytometry or various assay formats to block or measure integrin alpha 4 beta 7.

Entrez Gene IDs
3676 (Human); 16402 (Mouse)
Alternate Names
269C wild type; CD49 antigen-like family member D; CD49d antigen; CD49d; CD49d/beta7; CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor; IA4; Integrin alpha 4 beta 7; integrin alpha 4; integrin alpha-4 subunit; integrin alpha-4; Integrin alpha-IV; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); LPAM1; LPAM-1; MGC90518; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha

Product Datasheets

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

FAQs

  1. What is the epitope of Human Integrin alpha 4 beta 7/LPAM-1 (Research Grade Vedolizumab Biosimilar) Antibody, Catalog # MAB10078? 

    • We have not mapped the epitope of this Biosimilar antibody, but the variable sequence is the same as the therapeutic antibody Vedolizumab.  According to the following literature, Vedolizumab binds to the extracellular portion of Integrin beta 7: https://academic.oup.com/ecco-jcc/article/10/12/1437/2706991, and https://rupress.org/jcb/article/196/1/131/36697.

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Reviews for Human Integrin  alpha 4 beta 7/LPAM-1 (Research Grade Vedolizumab Biosimilar) Alexa Fluor® 647-conjugated Antibody

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Human Integrin  alpha 4 beta 7/LPAM‑1 (Research Grade Vedolizumab Biosimilar) Alexa Fluor® 647‑conjugated Antibody
By Anonymous on 10/17/2019
Application: Flow Sample Tested: Blood lymphocytes Species: Cynomolgus Monkey