Human Integrin  alpha 4 beta 7/LPAM‑1 (Research Grade Vedolizumab Biosimilar) PE‑conjugated Antibody

R&D Systems | Catalog # FAB10078P

Recombinant Monoclonal Antibody For Research Use Only
R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu117
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Product Specifications

Immunogen

Human Integrin alpha 4 beta 7/LPAM‑1

Specificity

Detects human Integrin alpha 4 beta 7/LPAM‑1 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Vedolizumab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Scientific Data Images

Detection of Integrin alpha 4 beta 7/LPAM-1 antibody in Human PBMC antibody by Flow Cytometry.

Detection of Integrin alpha 4 beta 7/LPAM-1 in Human PBMC by Flow Cytometry.

Human PBMC were stained with (A) Human Anti-Human Integrin alpha 4 beta 7/LPAM-1 (Research Grade Vedolizumab Biosimilar) PE-conjugated Monoclonal Antibody (Catalog # FAB10078P) or (B) no primary antibody, followed by Mouse anti-Human CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB100A). View our protocol for Staining Membrane-associated Proteins.

Applications

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human PBMC

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 4 beta 7/LPAM-1

Vedolizumab is a biosimilar that binds and blocks binds to integrin alpha 4 beta 7. This product can be used in flow cytometry or various assay formats to block or measure integrin alpha 4 beta 7.

Alternate Names

CD49d/beta7, LPAM-1, LPAM1

Entrez Gene IDs

3676 (Human); 16402 (Mouse)

Gene Symbol

ITGA4

Additional Integrin alpha 4 beta 7/LPAM-1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices

For research use only

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FAQs

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  • Q: What is the epitope of Human Integrin alpha 4 beta 7/LPAM-1 (Research Grade Vedolizumab Biosimilar) Antibody, Catalog # MAB10078? 

    A: We have not mapped the epitope of this Biosimilar antibody, but the variable sequence is the same as the therapeutic antibody Vedolizumab.  According to the following literature, Vedolizumab binds to the extracellular portion of Integrin beta 7: https://academic.oup.com/ecco-jcc/article/10/12/1437/2706991, and https://rupress.org/jcb/article/196/1/131/36697.

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