Human Integrin  alpha 4 beta 7/LPAM-1 (Research Grade Vedolizumab Biosimilar) Antibody

Recombinant Monoclonal Antibody For Research Use Only
Catalog # Availability Size / Price Qty
MAB10078-100
MAB10078-SP
Detection of Integrin  alpha 4 beta 7/LPAM‑1 in Human PBMC by Flow Cytometry.
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Human Integrin  alpha 4 beta 7/LPAM-1 (Research Grade Vedolizumab Biosimilar) Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha 4 beta 7/LPAM‑1 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Vedolizumab. This product is for research use only.
Source
Recombinant Monoclonal Human IgG1 Clone # Hu117
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human Integrin alpha 4 beta 7/LPAM‑1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Integrin a4 beta 7/LPAM-1 antibody in Human PBMC antibody by Flow Cytometry. View Larger

Detection of Integrin alpha 4 beta 7/LPAM‑1 in Human PBMC by Flow Cytometry. Human PBMC were stained with (A) Human Anti-Human Integrin a4 beta 7/LPAM-1 (Research Grade Vedolizumab Biosimilar) Monoclonal Antibody (Catalog # MAB10078) or (B) no primary antibody, followed by anti-Human IgG APC-conjugated Secondary Antibody (Catalog # F0135) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 4 beta 7/LPAM-1

Vedolizumab is a biosimilar that binds and blocks binds to integrin alpha 4 beta 7. This product can be used in flow cytometry or various assay formats to block or measure integrin alpha 4 beta 7.

Entrez Gene IDs
3676 (Human); 16402 (Mouse)
Alternate Names
269C wild type; CD49 antigen-like family member D; CD49d antigen; CD49d; CD49d/beta7; CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor; IA4; Integrin alpha 4 beta 7; integrin alpha 4; integrin alpha-4 subunit; integrin alpha-4; Integrin alpha-IV; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); LPAM1; LPAM-1; MGC90518; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha

Product Datasheets

FAQs

  1. Does this Biosimilar antibody have the same sequence as the therapeutic antibody?

    • The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody.

  2. What is a Biosimilar antibody?

    • A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

  3. What is the epitope of Human Integrin alpha 4 beta 7/LPAM-1 (Research Grade Vedolizumab Biosimilar) Antibody, Catalog # MAB10078? 

    • We have not mapped the epitope of this Biosimilar antibody, but the variable sequence is the same as the therapeutic antibody Vedolizumab.  According to the following literature, Vedolizumab binds to the extracellular portion of Integrin beta 7: https://academic.oup.com/ecco-jcc/article/10/12/1437/2706991, and https://rupress.org/jcb/article/196/1/131/36697.

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Reconstitution Buffers

Reviews for Human Integrin  alpha 4 beta 7/LPAM-1 (Research Grade Vedolizumab Biosimilar) Antibody

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Human Integrin  alpha 4 beta 7/LPAM-1 (Research Grade Vedolizumab Biosimilar) Antibody
By Anonymous on 10/17/2019
Application: Flow Sample Tested: Large intestine Species: Cynomolgus Monkey