Human Integrin alpha L/CD11a PerCP-conjugated Antibody

Name: Human Integrin alpha L/CD11a PerCP-conjugated Antibody
Brand: R&D Systems
SKU: FAB35951C

Catalog #: FAB35951C Datasheet / COA / SDS

Discontinued Product

FAB35951C has been discontinued.

View all Integrin alpha L/CD11a products.

Detection of Integrin alpha L/CD11a in Whole Blood Monocytes by Flow Cytometry.

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All Integrin alpha L/CD11a Antibodies

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Human Integrin alpha L/CD11a PerCP-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin alpha L/CD11a in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Integrin alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, alpha 6, alpha 8, alpha 9, alpha 10, alpha 11, alpha M, alpha V, alpha X, rhIntegrin beta 3, rhIntegrin alpha 6(x1)+ beta 4, alpha 7(x2)+ beta 1, alpha D+ beta 2, alpha E+ beta 7, recombinant mouse Integrin alpha 2b or alpha L is observed.

Source

Monoclonal Mouse IgG_2A Clone # CR38

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Fibronectin purified human monocytes

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Integrin alpha L/CD11a in Whole Blood Monocytes by Flow Cytometry. Whole blood monocytes were stained with Mouse Anti-Human Integrin aL/CD11a PerCP-conjugated Monoclonal Antibody (Catalog # FAB35951C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Integrin alpha L/CD11a

Integrin subunit alpha L/CD11a is a 180 kDa type I TM glycoprotein that interacts only with Integrin beta 2/CD18 to form LFA-1, a leukocyte adhesion protein which binds endothelial cell ICAM. Human Integrin alpha L contains a 1064 aa extracellular domain (ECD), a 20 aa TM sequence and a 58 aa cytoplasmic domain. The ECD contains seven repeats that form a beta-propeller structure and one inserted vWA domain (I domain) containing a metal ion-dependent adhesion site (MIDAS). Human and mouse Integrin alpha L ECD share 74% aa identity. A second isoform has a 53 aa insert in the ECD.

Entrez Gene IDs

3683 (Human); 16408 (Mouse); 308995 (Rat)

Alternate Names

CD11 antigen-like family member A; CD11a antigen; CD11a; CD11Aantigen CD11A (p180), lymphocyte function-associated antigen 1, alphapolypeptide; Integrin alpha L; integrin alpha-L; integrin gene promoter; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen1; alpha polypeptide); ITGAL; Leukocyte adhesion glycoprotein LFA-1 alpha chain; Leukocyte function-associated molecule 1 alpha chain; LFA-1 alpha; LFA-1; LFA1A; LFA-1A; Ly-15; Ly-21; lymphocyte function-associated antigen 1