Human Integrin beta 3/CD61 Biotinylated Antibody

Catalog #: BAF2266 Datasheet / COA / SDS

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BAF2266

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin beta 3/CD61 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin beta 3/CD61 in Western blots. In Western blots, less than 5% cross‑reactivity with recombinant human (rh) Integrin beta 1, rhIntegrin beta 2 and recombinant mouse Integrin beta 6 is observed under reducing conditions.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 3/CD61
Gly27-Asp718
Accession # P05106

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Integrin beta 3/CD61

Flow Cytometry

2.5 µg/10⁶ cells

Human peripheral blood mononuclear cells or platelets

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 3/CD61

Integrin beta 3, also called CD61, associates with the Integrin alpha IIb or alpha V subunits to form heterodimeric receptors for collagens, fibronectin, vitronectin, fibrinogen, Von willebrand factor or thrombospondin. alpha IIb/ beta 3, also known as GPIIb IIIa (Glycoprotein IIb-IIIa), is expressed on platelets. alpha V/ beta 3, also known as vitronectin receptor, is expressed on endothelial cells, some B cells, platelets and monocytes.

Entrez Gene IDs

3690 (Human); 16416 (Mouse); 29302 (Rat); 102117791 (Cynomolgus Monkey)

Alternate Names

CD61 antigen; CD61; GP3A; GP3Aplatelet glycoprotein IIIa; GPIIIA; GPIIIaintegrin beta-3; INGRB3; Integrin beta 3; integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); ITG B3; ITGB3; Platelet membrane glycoprotein IIIa