Human Integrin beta 3/CD61 Antibody

R&D Systems | Catalog # AF2266

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Flow Cytometry, Simple Western, CyTOF-ready

Cited:

Western Blot, Interferent Testing, Westen Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Integrin beta 3/CD61 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 3/CD61
Gly27-Asp718
Accession # P05106

Specificity

Detects human Integrin beta 3/CD61 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Integrin beta 2 and recombinant mouse (rm) Integrin beta 6 is observed and less than 1% cross-reactivity with rhIntegrin beta 1 and rmIntegrin  beta 4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Integrin beta 3/CD61 Antibody

Detection of Human Integrin beta 3/CD61 antibody by Western Blot.

Detection of Human Integrin beta 3/CD61 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Integrin beta 3/ CD61 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2266) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Integrin beta 3/CD61 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Integrin  beta 3/CD61 by Simple WesternTM.

Simple Western shows lysates of Exosome Standards (HT‑29) (NBP3-11685) and U‑87 MG human glioblastoma/astrocytoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Integrin  beta 3/CD61 at approximately 116 kDa (as indicated) using 20 µg/mL of Goat Anti-Human Integrin  beta 3/CD61 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2266). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Applications for Human Integrin beta 3/CD61 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells

Simple Western

20 µg/mL
Sample: Exosome Standards (HT-29) (Catalog # NBP3-11685) and U‑87 MG human glioblastoma/astrocytoma cell line

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and DU145 human prostate carcinoma cell line

Reviewed Applications

Read 3 reviews rated 4.7 using AF2266 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin beta 3/CD61

Integrin beta 3, also called CD61, associates with the Integrin alpha IIb or alpha V subunits to form heterodimeric receptors for collagens, fibronectin, vitronectin, fibrinogen, Von willebrand factor or thrombospondin. alpha IIb/ beta 3, also known as GPIIb IIIa (Glycoprotein IIb-IIIa), is expressed on platelets. alpha V/ beta 3, also known as vitronectin receptor, is expressed on endothelial cells, some B cells, platelets and monocytes.

Alternate Names

CD61, GP3A, GPIIIA, INGRB3, ITGB3

Entrez Gene IDs

3690 (Human); 16416 (Mouse); 29302 (Rat); 102117791 (Cynomolgus Monkey)

Gene Symbol

ITGB3

UniProt

P05106

Additional Integrin beta 3/CD61 Products

Product Documents for Human Integrin beta 3/CD61 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Integrin beta 3/CD61 Antibody

For research use only

Citations for Human Integrin beta 3/CD61 Antibody

Customer Reviews for Human Integrin beta 3/CD61 Antibody (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human Integrin  beta 3/CD61 Antibody
    Name: Nick Dordai
    Application: Immunocytochemistry
    Sample Tested: SUM159
    Species: Human
    Verified Customer | Posted 12/01/2022
    Immunofluorescence (IF): ITGB3 Antibody [AF2266 ] was used (1:100) for labeling and microscopy analysis of the distribution of ITGB3 proteins in SUM159 cells.
    Human Integrin beta 3/CD61 Antibody AF2266
  • Human Integrin  beta 3/CD61 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: human MDAMB-231 whole cell lysate and SUM-159PT
    Species: Human
    Verified Customer | Posted 10/20/2022
    Western Blot: whole cell lysates from MDA-MB-231 or SUM159PT cells was loaded with 50 ug/lane. 10% SDS-PAGE. ITGB3 Antibody (AF2266) was used for primary antibody: 1:1000, 4℃, overnight.
    Human Integrin beta 3/CD61 Antibody AF2266
  • Human Integrin  beta 3/CD61 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Human
    Verified Customer | Posted 09/14/2019
    Human Integrin beta 3/CD61 Antibody AF2266

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