Human Integrin beta 5 Alexa Fluor® 350-conjugated Antibody

Catalog #: AF3824U Datasheet / COA / SDS

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AF3824U-100UG

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Human Integrin beta 5 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin beta 5 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) Integrin beta 5 is observed, and less than 5% cross-reactivity with recombinant human (rh) Integrin&n

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO cell-derived recombinant human Integrin Î²5
Gly24-Asn719
Accession # P18084

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Integrin beta 5

Integrin beta 5 is a 100‑110 kDa type I transmembrane glycoprotein that forms a non-disulfide-linked heterodimer with Integrin alpha _V. Integrin alpha _V beta 5 is a receptor for vitronectin that is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, and select endothelium. The 696 amino acid residue Integrin beta 5 extracellular region contains a vWF-A domain and a cysteine-rich tandem repeat region. Human Integrin beta 5 shares 91% and 89% amino acid sequence homology with mouse and canine Integrin beta 5, respectively.

Entrez Gene IDs

3693 (Human); 16419 (Mouse); 257645 (Rat); 102123619 (Cynomolgus Monkey)

Alternate Names

FLJ26658; Integrin beta 5; integrin beta-5; integrin, beta 5; ITGB5