Human Integrin  beta 5 Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Integrin  beta 5 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) Integrin beta 5 is observed, and less than 5% cross-reactivity with recombinant human (rh) Integrin  beta 1, rhIntegrin  beta 3, rhIntegrin  beta 4, rhIntegrin  beta 6 and rmIntegrin  beta 7 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO cell-derived recombinant human Integrin beta 5
    Gly24-Asn719
    Accession # P18084
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Integrin  beta 5
  • Flow Cytometry
    2.5 µg/106 cells
    MG‑63 human osteosarcoma cell line
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Integrin  beta 5 in MG‑63 Human Cell Line. Integrin  beta 5 was detected in immersion fixed MG‑63 human osteosarcoma cell line using Sheep Anti-Human Integrin  beta 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3824) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 5

Integrin  beta 5 is a 100‑110 kDa type I transmembrane glycoprotein that forms a non-disulfide-linked heterodimer with Integrin  alpha V. Integrin  alpha V beta 5 is a receptor for vitronectin that is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, and select endothelium. The 696 amino acid residue Integrin  beta 5 extracellular region contains a vWF-A domain and a cysteine-rich tandem repeat region. Human Integrin  beta 5 shares 91% and 89% amino acid sequence homology with mouse and canine Integrin  beta 5, respectively.

  • Entrez Gene IDs:
    3693 (Human); 16419 (Mouse); 257645 (Rat)
  • Alternate Names:
    FLJ26658; Integrin beta 5; integrin beta-5; integrin, beta 5; ITGB5
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Targeting cadherin-17 inactivates Ras/Raf/MEK/ERK signaling and inhibits cell proliferation in gastric cancer.
    Authors: Lin Z, Zhang C, Zhang M, Xu D, Fang Y, Zhou Z, Chen X, Qin N, Zhang X
    PLoS ONE, 2014;9(1):e85296.
    Species: Human
    Sample Type: Whole Cells
    Application: WB
  2. Development of a method for the purification and culture of rodent astrocytes.
    Authors: Foo LC, Allen NJ, Bushong EA, Ventura PB, Chung WS, Zhou L, Cahoy JD, Daneman R, Zong H, Ellisman MH, Barres BA
    Neuron, 2011;71(5):799-811.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
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