Human Integrin  beta 5 Antibody

Catalog # Availability Size / Price Qty
AF3824
AF3824-SP
Integrin  beta 5 in MG‑63 Human Cell Line.
3 Images
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human Integrin  beta 5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin  beta 5 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) Integrin beta 5 is observed, and less than 5% cross-reactivity with recombinant human (rh) Integrin  beta 1, rhIntegrin  beta 3, rhIntegrin  beta 4, rhIntegrin  beta 6 and rmIntegrin  beta 7 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO cell-derived recombinant human Integrin beta 5
Gly24-Asn719
Accession # P18084
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
HT‑29 human colon adenocarcinoma cell line
Simple Western
50 µg/mL
MG‑63 human osteosarcoma cell line
Flow Cytometry
2.5 µg/106 cells
MG‑63 human osteosarcoma cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Integrin  beta 5 antibody in MG-63 Human Cell Line by Immunocytochemistry (ICC). View Larger

Integrin beta 5 in MG‑63 Human Cell Line. Integrin beta 5 was detected in immersion fixed MG-63 human osteosarcoma cell line using Sheep Anti-Human Integrin beta 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3824) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western View Larger

Detection of Human Integrin  beta 5 by Simple WesternTM. Simple Western lane view shows lysates of MG‑63 human osteosarcoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin  beta 5 at approximately 129 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human Integrin  beta 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3824) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot View Larger

Detection of Human Integrin  beta 5 by Western Blot. Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Integrin  beta 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3824) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Integrin  beta 5 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 5

Integrin  beta 5 is a 100‑110 kDa type I transmembrane glycoprotein that forms a non-disulfide-linked heterodimer with Integrin  alpha V. Integrin  alpha V beta 5 is a receptor for vitronectin that is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, and select endothelium. The 696 amino acid residue Integrin  beta 5 extracellular region contains a vWF-A domain and a cysteine-rich tandem repeat region. Human Integrin  beta 5 shares 91% and 89% amino acid sequence homology with mouse and canine Integrin  beta 5, respectively.

Entrez Gene IDs
3693 (Human); 16419 (Mouse); 257645 (Rat); 102123619 (Cynomolgus Monkey)
Alternate Names
FLJ26658; Integrin beta 5; integrin beta-5; integrin, beta 5; ITGB5

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Citations for Human Integrin  beta 5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing
    Authors: S Abbasi, S Sinha, E Labit, NL Rosin, G Yoon, W Rahmani, A Jaffer, N Sharma, A Hagner, P Shah, R Arora, J Yoon, A Islam, A Uchida, CK Chang, JA Stratton, RW Scott, FMV Rossi, TM Underhill, J Biernaskie
    Cell Stem Cell, 2020-08-04;27(3):396-412.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Targeting cadherin-17 inactivates Ras/Raf/MEK/ERK signaling and inhibits cell proliferation in gastric cancer.
    Authors: Lin Z, Zhang C, Zhang M, Xu D, Fang Y, Zhou Z, Chen X, Qin N, Zhang X
    PLoS ONE, 2014-01-20;9(1):e85296.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  3. Development of a method for the purification and culture of rodent astrocytes.
    Authors: Foo LC, Allen NJ, Bushong EA, Ventura PB, Chung WS, Zhou L, Cahoy JD, Daneman R, Zong H, Ellisman MH, Barres BA
    Neuron, 2011-09-08;71(5):799-811.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human Integrin  beta 5 Antibody

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Human Integrin  beta 5 Antibody
By Anonymous on 06/02/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human

MDA-MB-231 cells were growm on collagen coated coverslips for 12 hours.