Human Integrin  beta 5 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF3824

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Integrin  beta 5 in MCF‑7 Human Cell Line.
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Citations (1)
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Human Integrin  beta 5 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin beta 5 in Western blots. In Western blots, less than 5% cross-reactivity with Integrin beta 1, -beta 2, -beta 3, recombinant mouse Integrin beta 4, -beta 6, and -beta 7 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO cell-derived recombinant human Integrin beta 5
Gly24-Asn719
Accession # P18084
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Integrin  beta 5
Flow Cytometry
2.5 µg/106 cells
MG‑63 human osteosarcoma cell line
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Integrin  beta 5 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

Integrin beta 5 in MCF‑7 Human Cell Line. Integrin beta 5 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human Integrin beta 5 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3824) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to transmembrane proteins. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 5

Integrin  beta 5 is a 100‑110 kDa type I transmembrane glycoprotein that forms a non-disulfide-linked heterodimer with Integrin  alpha V. Integrin  alpha V beta 5 is a receptor for vitronectin that is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, and select endothelium. The 696 amino acid residue Integrin  beta 5 extracellular region contains a vWF-A domain and a cysteine-rich tandem repeat region. Human Integrin  beta 5 shares 91% and 89% amino acid sequence homology with mouse and canine Integrin  beta 5, respectively.

Entrez Gene IDs
3693 (Human); 16419 (Mouse); 257645 (Rat); 102123619 (Cynomolgus Monkey)
Alternate Names
FLJ26658; Integrin beta 5; integrin beta-5; integrin, beta 5; ITGB5

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Citation for Human Integrin  beta 5 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Integrin &alphav&beta5 heterodimer is a specific marker of human pancreatic beta cells
    Authors: JV Schiesser, T Loudovaris, HE Thomas, AG Elefanty, EG Stanley
    Scientific Reports, 2021-04-15;11(1):8315.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, ICC, IHC

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