Human Integrin  beta 5 Biotinylated Antibody

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Integrin  beta 5 in MCF‑7 Human Cell Line.
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Product Details
Citations (1)
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Human Integrin  beta 5 Biotinylated Antibody Summary

Species Reactivity
Detects human Integrin beta 5 in Western blots. In Western blots, less than 5% cross-reactivity with Integrin beta 1, -beta 2, -beta 3, recombinant mouse Integrin beta 4, -beta 6, and -beta 7 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
Chinese hamster ovary cell line CHO cell-derived recombinant human Integrin beta 5
Accession # P18084
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human Integrin  beta 5
Flow Cytometry
2.5 µg/106 cells
MG‑63 human osteosarcoma cell line
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Integrin  beta 5 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

Integrin beta 5 in MCF‑7 Human Cell Line. Integrin beta 5 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human Integrin beta 5 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3824) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to transmembrane proteins. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 5

Integrin  beta 5 is a 100‑110 kDa type I transmembrane glycoprotein that forms a non-disulfide-linked heterodimer with Integrin  alpha V. Integrin  alpha V beta 5 is a receptor for vitronectin that is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, and select endothelium. The 696 amino acid residue Integrin  beta 5 extracellular region contains a vWF-A domain and a cysteine-rich tandem repeat region. Human Integrin  beta 5 shares 91% and 89% amino acid sequence homology with mouse and canine Integrin  beta 5, respectively.

Entrez Gene IDs
3693 (Human); 16419 (Mouse); 257645 (Rat); 102123619 (Cynomolgus Monkey)
Alternate Names
FLJ26658; Integrin beta 5; integrin beta-5; integrin, beta 5; ITGB5

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Citation for Human Integrin  beta 5 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Integrin &alphav&beta5 heterodimer is a specific marker of human pancreatic beta cells
    Authors: JV Schiesser, T Loudovaris, HE Thomas, AG Elefanty, EG Stanley
    Scientific Reports, 2021-04-15;11(1):8315.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, ICC, IHC


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