Human Integrin beta 7 (also Integrin beta P and M290 IEL Ag) is a 120 kDa member of the Integrin beta chain family of molecules. It is a type I transmembrane glycoprotein that contains a 704 amino acid (aa) extracellular domain (ECD) (aa 20-723) and a 52 aa cytoplasmic tail. The ECD contains one von Willebrand factor A domain (aa 150-389) and four cysteine-rich EGF-like repeats. One splice variant exists that shows a deletion of aa 501-648. Integrin beta 7 forms a nondisulfide linked heterodimer with alpha 4 and alpha E integrins. alpha 4 beta 7 binds fibronectin, VCAM-1, and MADCAM1, while alpha E beta 7 binds E-cadherin. The ECD of human beta 7 is 87% aa identical to the ECD of mouse beta 7.
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 7 long isoform
Glu20-His723
Accession # P26010
Glu20-His723
Accession # P26010
Specificity
Detects human Integrin beta 7 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Integrin beta 7 is observed and less than 1% cross-reactivity with recombinant human (rh) Integrin beta 2, rhIntegrin beta 5, and rhIntegrin beta 6 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Integrin beta 7 Antibody
Detection of Integrin beta 7 in Human Lymphocytes by Flow Cytometry.
Human blood derived lymphocytes were stained with Human Integrin beta 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4669, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).
Applications for Human Integrin beta 7 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human lymphocytes
Sample: Human lymphocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Human Integrin beta 7
Sample: Recombinant Human Integrin beta 7
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Integrin beta 7
Alternate Names
ITGB7
Gene Symbol
ITGB7
UniProt
Additional Integrin beta 7 Products
Product Documents for Human Integrin beta 7 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Integrin beta 7 Antibody
For research use only
Related Research Areas
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