Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all IQGAP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IQGAP1
Lys505-Ser717
Accession # P46940

Specificity

Detects endogenous human IQGAP1 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human IQGAP1 Antibody

Detection of Human IQGAP1 antibody by Western Blot.

Detection of Human IQGAP1 by Western Blot.

Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HepG2 human hepatocellular carcinoma cell line, U937 human histiocytic lymphoma cell line, and MRC-5 human embryonic lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human IQGAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5069) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IQGAP1 at approximately 195 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human IQGAP1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HUVEC human umbilical vein endothelial cells, HepG2 human hepatocellular carcinoma cell line, U937 human histiocytic lymphoma cell line, and MRC-5 human embryonic lung fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IQGAP1

IQGAP1 (IQ motif with Ras GAP-related domain 1) is a 195 kDa member of the IQGAP family of proteins. It serves as an effector protein for Rho family members, and regulates actin polymerization. Human IQGAP1 is 1657 amino acids (aa) in length. It contains one actin-binding CH domain (aa 45‑159), a Pro-binding WWP region (aa 685‑711), four consecutive calmodulin-binding IQ motifs (aa 745‑864), and a noncatalytic Ras-GAP domain (aa 1004‑1237). Amino acids 763‑863 mediate homodimerization. There are at least three IQGAP1 splice variants. One shows a two Lys substitution for aa 270‑1657, a second shows a ten Lys substitution for aa 299‑1657, and a third shows a three Lys substitution for aa 941‑1657.

Long Name

IQ Motif Containing GTPase Activating Protein 1

Alternate Names

p195, SAR1

Entrez Gene IDs

8826 (Human); 544963 (Mouse)

Gene Symbol

IQGAP1

UniProt

P46940

Additional IQGAP1 Products

Product Documents for Human IQGAP1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IQGAP1 Antibody

For research use only

Related Research Areas

Cancer Biomarkers

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