Human IQGAP1 Antibody

Catalog # Availability Size / Price Qty
AF5069
AF5069-SP
Detection of Human IQGAP1 by Western Blot.
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Human IQGAP1 Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human IQGAP1 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IQGAP1
Lys505-Ser717
Accession # P46940
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human IQGAP1 antibody by Western Blot. View Larger

Detection of Human IQGAP1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HepG2 human hepatocellular carcinoma cell line, U937 human histiocytic lymphoma cell line, and MRC-5 human embryonic lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human IQGAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5069) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IQGAP1 at approximately 195 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IQGAP1

IQGAP1 (IQ motif with Ras GAP-related domain 1) is a 195 kDa member of the IQGAP family of proteins. It serves as an effector protein for Rho family members, and regulates actin polymerization. Human IQGAP1 is 1657 amino acids (aa) in length. It contains one actin-binding CH domain (aa 45‑159), a Pro-binding WWP region (aa 685‑711), four consecutive calmodulin-binding IQ motifs (aa 745‑864), and a noncatalytic Ras-GAP domain (aa 1004‑1237). Amino acids 763‑863 mediate homodimerization. There are at least three IQGAP1 splice variants. One shows a two Lys substitution for aa 270‑1657, a second shows a ten Lys substitution for aa 299‑1657, and a third shows a three Lys substitution for aa 941‑1657.

Long Name
IQ Motif Containing GTPase Activating Protein 1
Entrez Gene IDs
8826 (Human); 544963 (Mouse)
Alternate Names
HUMORFA01; IQ motif containing GTPase activating protein 1; IQGAP1; KIAA0051ras GTPase-activating-like protein IQGAP1; p195; p195RasGAP-like with IQ motifs; SAR1

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