Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all ITM2A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ITM2A
Pro80-Glu263
Accession # O43736

Specificity

Detects human ITM2A in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) ITM2B is observed, and less than 1% cross-reactivity with rhITM2C is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human ITM2A Antibody

Detection of Human ITM2A by Western Blot.

Western Blot shows lysates of A‑673 human Ewing's sarcoma cell line. PVDF membrane was probed with 2 µg/ml of Sheep Anti-Human ITM2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4876) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ITM2A at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human ITM2A Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: A-673 human Ewing's sarcoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ITM2A

ITM2A (Integral membrane protein 2A; also protein E25) is a 43-45 kDa member of the ITM2 family of proteins. It is expressed by chondrocytes involved in endochondrial ossification, skeletal muscle, adipose tissue-derived stem cells, and CD3-activated CD4+ and CD8+ T cells. ITM2A is found embedded in both plasma membrane and Golgi complex. Human ITM2A is a 263 amino acid (aa) type II transmembrane glycoprotein. The ECD (aa 75-263) contains one BRICHOS domain (aa 133‑227). Proteolytic cleavage by furin is typical for BRICHOS domain-containing proteins. ITM2A may exist as a dimer. There are two potential splice variants, one that shows a deletion of aa 55-81, and a second that shows a deletion of aa 38-81. Full-length human ITM2A shares 95% aa identity with mouse ITM2A.

Long Name

Integral Membrane Protein 2A

Alternate Names

BRICD2A, E25A

Entrez Gene IDs

9452 (Human); 16431 (Mouse); 317218 (Rat)

Gene Symbol

ITM2A

UniProt

O43736

Additional ITM2A Products

Product Documents for Human ITM2A Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ITM2A Antibody

For research use only

Related Research Areas

Chondrogenesis Markers

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