Human Jak3 Antibody

Catalog # Availability Size / Price Qty
Detection of Human Jak3 by Western Blot.
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Product Details
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Reviews (2)

Human Jak3 Antibody Summary

Species Reactivity
Detects human Jak3 in Western blots.
Monoclonal Mouse IgG2B Clone # 452506
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Jak3
Accession # P52333
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Jak3 antibody by Western Blot. View Larger

Detection of Human Jak3 by Western Blot. Western blot shows lysates of TF-1 human erythroleukemic cell line. PVDF membrane was probed with 2 µg/mL of Human Jak3 Monoclonal Antibody (Catalog # MAB4699) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Jak3 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Jak3

Janus Kinase 3 (Jak3) belongs to the Jak family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Also known as Leukocyte Janus Kinase (LJak), Jak3 is activated after binding to the gamma chain of Interleukin receptors, specifically the IL-2 and IL-4 receptors. Activation of Jak3 is associated with the rapid tyrosine phosphorylation of STAT proteins. Defects in Jak3 cause an autosomal severe combined immunodeficiency disease (SCID).

Entrez Gene IDs
3718 (Human); 16453 (Mouse); 25326 (Rat)
Alternate Names
EC 2.7.10; Jak3; JAK3_HUMAN; JAK-3EC; JAKL; JAKLtyrosine-protein kinase JAK3; Janus kinase 3Leukocyte janus kinase; LJAK; L-JAKJanus kinase 3 (a protein tyrosine kinase, leukocyte)

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Human Jak3 Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human Jak3 Antibody
By Anonymous on 11/02/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human