Human Jak3 Antibody

R&D Systems | Catalog # MAB4699

R&D Systems
Discontinued Product
MAB4699 has been discontinued. View all Jak3 products.

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 452506
View all Jak3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Jak3
Gly46-Thr209
Accession # P52333

Specificity

Detects human Jak3 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Jak3 Antibody

Detection of Human Jak3 antibody by Western Blot.

Detection of Human Jak3 by Western Blot.

Western blot shows lysates of TF-1 human erythroleukemic cell line. PVDF membrane was probed with 2 µg/mL of Human Jak3 Monoclonal Antibody (Catalog # MAB4699) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Jak3 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human Jak3 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: TF-1 human erythroleukemic cell line

Reviewed Applications

Read 2 reviews rated 3.5 using MAB4699 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Jak3

Janus Kinase 3 (Jak3) belongs to the Jak family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Also known as Leukocyte Janus Kinase (LJak), Jak3 is activated after binding to the gamma chain of Interleukin receptors, specifically the IL-2 and IL-4 receptors. Activation of Jak3 is associated with the rapid tyrosine phosphorylation of STAT proteins. Defects in Jak3 cause an autosomal severe combined immunodeficiency disease (SCID).

Alternate Names

JAKL, LJAK

Entrez Gene IDs

3718 (Human); 16453 (Mouse); 25326 (Rat)

Gene Symbol

JAK3

UniProt

P52333

Additional Jak3 Products

Product Documents for Human Jak3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Jak3 Antibody

For research use only

Citations for Human Jak3 Antibody

Customer Reviews for Human Jak3 Antibody (2)

3.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
50%
3 Stars
50%
2 Stars
0%
1 Stars
0%

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Customer Images

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  • Human Jak3 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
    Human Jak3 Antibody MAB4699
  • Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/02/2018

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