Human Kallikrein 13 Antibody Summary
Accession # Q9UKR3
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Kallikrein 13 in Human Prostate Cancer Tissue. Kallikrein 13 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Goat Anti-Human Kallikrein 13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2625) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kallikrein 13
Human tissue Kallikrein 13 (hK13) is a member of the human tissue kallikrein family. The gene encoding for rhK13 is termed KLK13 and it resides on chromosome 19q13.3-4 along with fourteen other members of the family. KLK13 spans about 8.7 kb genomic DNA and the longest transcript is 831 bp, encoding for a protein of 277 amino acids (1). Another five shorter splice variants have also been identified. They are specifically expressed in the testicular tissue and encode for five truncated forms of hK13 (2). Due to the aspartic acid residue in the substrate binding pocket, the enzymatic activity of hK13 is predicted be trypsin-like. It has been shown that recombinant hK13 produced in yeast can cleave synthetic peptides after the arginine residue and some extracellular matrix components (3). However, its exact physiological substrates and functions remain obscure. Despite the lack of knowledge on the physiological function of hK13, several studies have demonstrated that hK13 is implicated with cancer of the breast and ovary and it can serve as a favorable prognostic biomarker for these malignancies (4, 5). The purified, secreted rhK13 is mainly the pro form. When activated by lysyl-endopeptidase, it displays enzymatic activity towards a fluorogenic synthetic peptide. This activity can be inhibited by rhSerpin A5, E1, and F2 (R&D Systems, Catalog # 1266-PI, 1786-PI, and 1470-PI).
- Yousef, G.M. et al. (2000) J. Biol. Chem. 275:11891.
- Chang, A. et al. (2001) Anticancer Res. 21:3147.
- Kapadia, C. et al. (2004) Biochem. Biophys. Res. Commun. 323:1084.
- Chang, A. et al. (2002) Br. J. Cancer. 86:1457.
- Scorilas, A. et al. (2004) J. Clin. Oncol. 22:678.
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