Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Neutralization, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Kallikrein 13 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 13
Val19-Ile262
Accession # Q9UKR3

Specificity

Detects human Kallikrein 13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) Kallikrein 8 and less than 5% cross-reactivity with rhKallikrein 11, rhKallikrein 14, and rhKallikrein 15 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Kallikrein 13 Antibody

Kallikrein 13 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P).

Kallikrein 13 in Human Prostate Cancer Tissue.

Kallikrein 13 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Goat Anti-Human Kallikrein 13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2625) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Kallikrein 13 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Kallikrein 13 (Catalog # 2625-SE), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Kallikrein 13 (Catalog # 2625-SE)

Neutralization

Measured by its ability to neutralize Recombinant Human Kallikrein 13 (1 µg/mL, Catalog # 2625-SE) cleavage of the fluorogenic peptide substrate Boc-VPR-AMC (100 µM, Catalog # ES011). The Neutralization Dose (ND50) is typically 8.6 µg/mL.

Reviewed Applications

Read 2 reviews rated 4.5 using AF2625 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Kallikrein 13

Human tissue Kallikrein 13 (hK13) is a member of the human tissue kallikrein family. The gene encoding for rhK13 is termed KLK13 and it resides on chromosome 19q13.3-4 along with fourteen other members of the family. KLK13 spans about 8.7 kb genomic DNA and the longest transcript is 831 bp, encoding for a protein of 277 amino acids (1). Another five shorter splice variants have also been identified. They are specifically expressed in the testicular tissue and encode for five truncated forms of hK13 (2). Due to the aspartic acid residue in the substrate binding pocket, the enzymatic activity of hK13 is predicted be trypsin-like. It has been shown that recombinant hK13 produced in yeast can cleave synthetic peptides after the arginine residue and some extracellular matrix components (3). However, its exact physiological substrates and functions remain obscure. Despite the lack of knowledge on the physiological function of hK13, several studies have demonstrated that hK13 is implicated with cancer of the breast and ovary and it can serve as a favorable prognostic biomarker for these malignancies (4, 5). The purified, secreted rhK13 is mainly the pro form. When activated by lysyl-endopeptidase, it displays enzymatic activity towards a fluorogenic synthetic peptide. This activity can be inhibited by rhSerpin A5, E1, and F2 (R&D Systems, Catalog # 1266-PI, 1786-PI, and 1470-PI).

References

  1. Yousef, G.M. et al. (2000) J. Biol. Chem. 275:11891.
  2. Chang, A. et al. (2001) Anticancer Res. 21:3147.
  3. Kapadia, C. et al. (2004) Biochem. Biophys. Res. Commun. 323:1084.
  4. Chang, A. et al. (2002) Br. J. Cancer. 86:1457.
  5. Scorilas, A. et al. (2004) J. Clin. Oncol. 22:678.

Alternate Names

KLK13

Entrez Gene IDs

26085 (Human)

Gene Symbol

KLK13

UniProt

Q9UKR3

Additional Kallikrein 13 Products

Product Documents for Human Kallikrein 13 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Kallikrein 13 Antibody

For research use only

Customer Reviews for Human Kallikrein 13 Antibody (2)

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  • Human Kallikrein 13 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 07/05/2022
    works well for ELISA in combination with mAb
    Human Kallikrein 13 Antibody AF2625
  • Human Kallikrein 13 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: mucociliary epithelium
    Species: Human
    Verified Customer | Posted 04/02/2020
    Human Kallikrein 13 Antibody AF2625

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