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Human KIR2DL3/CD158b2 PerCP-conjugated Antibody

Catalog #: FAB2014C
1 Citation Datasheet / COA / SDS

Discontinued Product

FAB2014C has been discontinued.
View all KIR2DL3/CD158b2 products.
Detection of KIR2DL3/CD158b2 in Human Blood Lymphocytes by Flow Cytometry.
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Human KIR2DL3/CD158b2 PerCP-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human KIR2DL3/CD158b2. Stains BaF3 cells transfected with human KIR2DL3 in flow cytometry. Does not stain BaF3 cells transfected with KIR2DL1, 2DL2, 2DL4, 2DL5, 2DS1, 2DS2, 2DS4, 3DL1, 3DL2, or 3DS1.
Source
Monoclonal Mouse IgG2A Clone # 180701
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human KIR2DL3/CD158b2
Accession # P43628
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of KIR2DL3/CD158b2 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of KIR2DL3/CD158b2 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human KIR2DL3/CD158b2 PerCP-conjugated Monoclonal Antibody (Catalog # FAB2014C) or (B) Mouse IgG2APerCP Isotype Control (Catalog # IC003C). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KIR2DL3/CD158b2

KIR2DL3 is one of several immunoglobulin-like receptors expressed on NK and T cells that bind MHC class I molecules and regulate killer cell activity. KIR2DL3 interacts specifically with HLA-Cw3.

Long Name
Killer Cell Immunoglobulin-like Receptor, Two Domain Long Cytoplasmic Tail, 3
Entrez Gene IDs
3804 (Human)
Alternate Names
CD158 antigen-like family member B2; CD158b2 antigen; CD158b2; CD158B2CD158b; cl-6; killer cell immunoglobulin-like receptor 2DL3; killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 3; Killer inhibitory receptor cl 2-3; KIR-023GB; KIR2DL3; KIRCL23; KIR-K7b; KIR-K7c; MGC129943; MHC class I NK cell receptor; natural killer associated transcript 2; natural killer cell inhibitory receptor KIR2DL3; Natural killer-associated transcript 2; NKAT; NKAT2; NKAT-2; NKAT2A; NKAT2b; NKAT2GL183; NK-receptor; p58 natural killer cell receptor clone CL-6; p58 NK receptor CL-6; p58; p58.2 MHC class-I specific NK receptor; p58.2 MHC class-I-specific NK receptor

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Citation for Human KIR2DL3/CD158b2 PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Licensed human natural killer cells aid dendritic cell maturation via TNFSF14/LIGHT.
    Authors: Holmes T, Wilson E, Black E, Benest A, Vaz C, Tan B, Tanavde V, Cook G
    Proc Natl Acad Sci U S A, 2014-12-15;111(52):E5688-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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