Human Kirrel2/NEPH3 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF2564
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Human Kirrel2/NEPH3 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Kirrel2/NEPH3 in Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant mouse Kirrel2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kirrel2/NEPH3
Gly21-Arg503
Accession # Q6UWL6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Kirrel2/NEPH3 (Catalog # 2564-K2)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kirrel2/NEPH3

Kirrel2 (kin of irregular chiasm-1-like 2), also known as filtrin, NEPH3 (nephrin-like 3) or NLG1 (nephrin-like gene 1), is a 107 kDa type I transmembrane adhesion protein belonging to the immunoglobulin (Ig) superfamily (1, 2). Kirrel2 is expressed both in pancreatic islet beta  cells and kidney glomerulus, and is also found in brain and lymph nodes (1‑9). Human Kirrel2 cDNA encodes a 708 amino acid (aa) protein that contains a 19 aa signal sequence, a 491 aa extracellular domain with five C2‑type Ig-like domains, a 21 aa transmembrane sequence and a 177 aa intracellular domain with a nephrin-like podocin binding site (aa 597‑606) (4, 5). An extracellular RGD sequence allows for cell attachment (4). The extracellular domain of human Kirrel2 shares 86%, 84%, 89% and 87% aa identity with mouse, rat, equine and bovine Kirrel2, respectively. Alternately spliced human Kirrel2 isoforms of 688, 633 and 583 aa have been described (6). These are, respectively, either missing aa 192‑211, or have a 23 aa alternate C‑terminus in place of aa 611‑708, with or without aa 22‑71 at the N‑terminus. Human Kirrel2 is downregulated in glomeruli of patients with proteinuria, suggesting an involvement in the maintenance of the glomerular filtration barrier (7). Autoantibodies to Kirrel2 have been identified in a subset of humans with type I diabetes, indicating its involvement with islet  beta cell function (8). In the brain, Kirrel2 is present in olfactory neurons, but only when they are active; homophilic adhesion of Kirrel2 is postulated to help correlate nerves identifying the same odorant (9). It is also a marker for mouse early postmitotic neural precursors in the ventricular zone of the developing mouse spinal cord (10).

Long Name
Kin of Irregular Chiasm-like 2, Splice Variant A
Entrez Gene IDs
84063 (Human); 243911 (Mouse)
Alternate Names
DKFZp564A1164; Filtrin; FILTRINkin of IRRE-like protein 2; kin of IRRE like 2 (Drosophila); Kin of irregular chiasm-like protein 2; Kirrel2; MGC15718; NEPH3; NEPH3Nephrin-like protein 3; nephrin-like 3; nephrin-like gene 1; NLG1; NLG1kin of irregular chiasm-like 2

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