Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all KLF10 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human KLF10
Asn31-Ser154
Accession # Q13118

Specificity

Detects human KLF10 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human KLF12 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human KLF10 Antibody

Detection of Human KLF10 antibody by Western Blot.

Detection of Human KLF10 by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and IMR-32 human neuroblastoma cell line and cytoplasmic extracts of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human KLF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7016) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for KLF10 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human KLF10 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, SH‑SY5Y human neuroblastoma cell line, and IMR‑32 human neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KLF10

KLF10 (Kruppel-like factor 10; also TIEG1 and EGR-alpha ) is a 55-65 kDa member of the Sp1 C2H2-type zinc-finger protein family. It appears to be very widely expressed, and functions as a transcriptional repressor in a noncovalent complex with JARID1B. KLF10 is induced by TGF-beta, GDNF and estrogen, and inhibits transcription of genes such as BI-1, OPG and SMAD7. By suppressing SMAD7, it promotes TGF-beta signaling. Human KLF10 is 480 amino acids (aa) in length and contains three C2H2-type zinc-finger motifs (aa 369-451). There is one potential alternate splice form that shows a deletion of aa 2-11. Over aa 31-154, human KLF10 shares 79% aa identity with mouse KLF10/mGIF.

Long Name

Kruppel-Like Factor 10

Alternate Names

EGR-alpha, TIEG1

Entrez Gene IDs

7071 (Human); 21847 (Mouse); 81813 (Rat)

Gene Symbol

KLF10

UniProt

Q13118 (Human)

Additional KLF10 Products

Product Documents for Human KLF10 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human KLF10 Antibody

For research use only

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