Human KLF10 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human KLF10 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human KLF12 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human KLF10
    Asn31-Ser154
    Accession # Q13118
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human KLF10 by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line and IMR‑32 human neuroblastoma cell line and cytoplasmic extracts of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human KLF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7016) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for KLF10 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: KLF10

KLF10 (Kruppel-like factor 10; also TIEG1 and EGR-alpha ) is a 55-65 kDa member of the Sp1 C2H2-type zinc-finger protein family. It appears to be very widely expressed, and functions as a transcriptional repressor in a noncovalent complex with JARID1B. KLF10 is induced by TGF-beta, GDNF and estrogen, and inhibits transcription of genes such as BI-1, OPG and SMAD7. By suppressing SMAD7, it promotes TGF-beta signaling. Human KLF10 is 480 amino acids (aa) in length and contains three C2H2-type zinc-finger motifs (aa 369-451). There is one potential alternate splice form that shows a deletion of aa 2-11. Over aa 31-154, human KLF10 shares 79% aa identity with mouse KLF10/mGIF.

  • Long Name:
    Kruppel-Like Factor 10
  • Entrez Gene IDs:
    7071 (Human); 21847 (Mouse); 81813 (Rat)
  • Alternate Names:
    EGRA; EGR-alpha; KLF10; Krueppel-like factor 10; Kruppel-like factor 10; TGFB inducible early growth response; TGFB-inducible early growth response protein 1; TIEG1; TIEG-1; TIEG1TIEG; Transforming growth factor-beta-inducible early growth response protein 1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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