Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all KLF17 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human KLF17
Met1-Gln228
Accession # Q5JT82

Specificity

Detects human KLF17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) KLF1, rhKLF2, rhKLF4, rhKLF5, rhKLF6, rhKLF10, and rhKLF12 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human KLF17 Antibody

Detection of Human KLF17 antibody by Western Blot.

Detection of Human KLF17 by Western Blot.

Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human KLF17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6845) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for KLF17 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human KLF17 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human testis tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KLF17

KLF17 (Kruppel-like factor 17) is a 38-39 kDa member of the Sp1 C2H2-type zinc-finger protein family. Based on mouse studies, it is expressed in germ cells and likely plays a role in spermatid and oocyte differentiation. It is also found to be downregulated in tumors where, if present, it normally suppresses Id1 (Inhibitor of DNA binding 1), a transcription factor associated with tumorigenesis. Human KLF17 is 389 amino acids (aa) in length and contains three zinc-finger motifs (aa 283‑365). There is one potential alternate splice form that shows a 19 aa substitution for aa 97-389. Over aa 1-228, human KLF17 shares only 33% aa identity with mouse ZFP393/KLF17.

Long Name

Kruppel-Like Factor 17

Alternate Names

Zfp393, ZNF393

Entrez Gene IDs

128209 (Human); 75753 (Mouse); 298449 (Rat)

Gene Symbol

KLF17

UniProt

Q5JT82 (Human)

Additional KLF17 Products

Product Documents for Human KLF17 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human KLF17 Antibody

For research use only

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