Human KLF17 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human KLF17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) KLF1, rhKLF2, rhKLF4, rhKLF5, rhKLF6, rhKLF10, and rhKLF12 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human KLF17
    Met1-Gln228
    Accession # Q5JT82
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human KLF17 by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human KLF17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6845) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for KLF17 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: KLF17

KLF17 (Kruppel-like factor 17) is a 38-39 kDa member of the Sp1 C2H2-type zinc-finger protein family. Based on mouse studies, it is expressed in germ cells and likely plays a role in spermatid and oocyte differentiation. It is also found to be downregulated in tumors where, if present, it normally suppresses Id1 (Inhibitor of DNA binding 1), a transcription factor associated with tumorigenesis. Human KLF17 is 389 amino acids (aa) in length and contains three zinc-finger motifs (aa 283‑365). There is one potential alternate splice form that shows a 19 aa substitution for aa 97-389. Over aa 1-228, human KLF17 shares only 33% aa identity with mouse ZFP393/KLF17.

  • Long Name:
    Kruppel-Like Factor 17
  • Entrez Gene IDs:
    128209 (Human); 75753 (Mouse); 298449 (Rat)
  • Alternate Names:
    FLJ40160; KLF17; Kruppel-like factor 17; Zfp393; Zinc finger protein 393Krueppel-like factor 17; ZNF393; ZNF393novel zinc-finger protein
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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