Human Kynureninase Antibody Summary
Met1-Asn465
Accession # Q16719
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Kynureninase by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Kynureninase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4887) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Kynureninase at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kynureninase
Kynureninase is a pyridoxal-5 (-phosphate-dependent enzyme that catalyzes the hydrolytic cleavage of the amino acids L-kynurenine and L-3-hydroxykynurenine to give either anthranilic acid or 3-hydroxyanthranilic acid and alanine (1). The enzyme is a member of the “kynurenine pathway” enzymes, through which the majority of dietary tryptophan is degraded in the liver, and is involved in the de novo biosynthesis of NAD+ (2, 3). Kynurenine pathway genes are expressed in immune system cells such as macrophages and microglia. During inflammatory responses, the kynurenine pathway in these cells produces quinolinic acid (QA) and not NAD+. QA excites neurons via the activation of NMDA (N-methyl-D-aspartate) receptors resulting in neuronal damage. The tissue-damaging process has been demonstrated in AIDS-related dementia complex, Alzheimer’s, stroke, epilepsy, and Huntington’s disease. Because Kynureninase is one of the key enzymes of QA production, its inhibitors may be useful for the treatment of neurological disorders. The recombinant Kynureninase has been shown to possess specificity for 3-hydroxykynurenine over kynurenine (4, 5).
- Lima, S. et al. (2007) Biochemistry 46:2735.
- Botting, N. P. (1995) Chem. Soc. Rev. 24:401.
- Stone, T. W. (2000) Trends in Pharm. Sci. 21:149.
- Walsh, H. et al. (2002) Eur. J. Chem. 269:2069.
- Toma, S. et al. (1997) FEBS Lett. 408:5.
Product Datasheets
Citations for Human Kynureninase Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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HIV-1 Envelope Mimicry of Host Enzyme Kynureninase Does Not Disrupt Tryptophan Metabolism
Authors: Barton F Haynes
J. Immunol., 2016-11-14;0(0):.
Applications: Neutralization -
Identification of autoantigens recognized by the 2F5 and 4E10 broadly neutralizing HIV-1 antibodies.
Authors: Yang, Guang, Holl, T Matt, Liu, Yang, Li, Yi, Lu, Xiaozhi, Nicely, Nathan I, Kepler, Thomas B, Alam, S Munir, Liao, Hua-Xin, Cain, Derek W, Spicer, Leonard, VandeBerg, John L, Haynes, Barton F, Kelsoe, Garnett
J Exp Med, 2013-01-28;210(2):241-56.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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