Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 4G8
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Product Specifications

Immunogen

Recombinant human L‑Selectin/CD62L Extracellular domain

Specificity

Binds to CHO cells expressing the human L‑Selectin/CD62L protein. It does not recognize CHO cells expressing either the human E-Selectin or human P-Selectin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human L‑Selectin/CD62L PE‑conjugated Antibody

Detection of L‑Selectin/CD62L in PBMC with CD3 costain cells by Flow Cytometry.

PBMCs were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human L‑Selectin/CD62L PE‑conjugated Monoclonal Antibody (Catalog # FAB9787P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Detection of L‑Selectin/CD62L in PBMCs with CD45RA costain by Flow Cytometry.

PBMCs were stained with Mouse Anti-Human CD45RA MFV610‑conjugated Monoclonal Antibody (Catalog # FAB11444MFV610) and either (A) Mouse Anti-Human L‑Selectin/CD62L PE‑conjugated Monoclonal Antibody (Catalog # FAB9787P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human L‑Selectin/CD62L PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: PBMCs with CD3 costain or PBMCs with CD45RA costain

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: L-Selectin/CD62L

L-Selectin (LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L), is a cell surface glycoprotein expressed constitutively on a wide variety of leukocytes. It plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites.

Alternate Names

CD62L, hLHRc, LAM1, LECAM1, Leu-8, LNHR, LSEL, Lyam-1, LYAM1, PLNHR, SELL, TQ1

Entrez Gene IDs

6402 (Human); 20343 (Mouse); 29259 (Rat); 102142895 (Cynomolgus Monkey)

Gene Symbol

SELL

Additional L-Selectin/CD62L Products

Product Documents for Human L‑Selectin/CD62L PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human L‑Selectin/CD62L PE‑conjugated Antibody

For research use only

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