Human LAMP-1/CD107a Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
IC4800T-100UG
Detection of LAMP-1/CD107a in THP‑1 Human Cell Line by Flow Cytometry.
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Human LAMP-1/CD107a Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human LAMP1/CD107a in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 508921
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LAMP1/CD107a
Ala28-Asn380
Accession # P11279
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of LAMP-1/CD107a in THP‑1 Human Cell Line by Flow Cytometry. View Larger

Detection of LAMP-1/CD107a in THP‑1 Human Cell Line by Flow Cytometry. THP‑1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human LAMP-1/CD107a Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # IC4800T, filled histogram) or isotype control antibody (Catalog # IC0041T, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LAMP-1/CD107a

Lysosome-associated membrane protein-1 (LAMP1), also known as CD107a, is a 100‑130 kDa member of the LAMP family of glycoproteins. It is expressed in lysosomal and plasma membranes of macrophages, NK and T-cells, and with LAMP2, is essential for the formation of phagolysosomes. On the cell surface, it also presents carbohydrates to selectins. Mature human LAMP1 is a 389 amino acid (aa) type I transmembrane glycoprotein. It contains a 354 aa luminal/extracellular domain (ECD) (aa 28‑381) and a 12 aa cytoplasmic tail (aa 405‑416). The ECD has two large looping regions (aa 28‑193 and 227‑381) plus multiple N- and O-linked glycosylation sites. There is one potential splice variant that shows a 26 aa substitution in the signal sequence. Over aa 28‑380, human LAMP1 shares 64% aa identity with mouse LAMP1.

Long Name
Lysosome-associated Membrane Glycoprotein 1
Entrez Gene IDs
3916 (Human); 16783 (Mouse)
Alternate Names
CD107 antigen-like family member A; CD107a antigen; CD107a; LAMP1; LAMP-1; LAMPA; LGP120; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1

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