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Human LAMP-1/CD107a Alexa Fluor® 594-conjugated Antibody

    
Datasheet / CoA / SDS
Detection of LAMP-1/CD107a in THP‑1 Human Cell Line by Flow Cytometry.

Intracellular Staining by Flow Cytometry

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  • Species Reactivity
    Human
  • Specificity
    Detects human LAMP1/CD107a in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 508921
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human LAMP1/CD107a
    Ala28-Asn380
    Accession # P11279
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 594
Product Datasheets
Product Datasheet

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SDS
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Intracellular Staining by Flow Cytometry
Intracellular Staining by Flow Cytometry LAMP-1/CD107a Antibody (508921) [Alexa Fluor® 594]
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Detection of LAMP-1/CD107a in THP‑1 Human Cell Line by Flow Cytometry. THP‑1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human LAMP-1/CD107a Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # IC4800T, filled histogram) or isotype control antibody (Catalog # IC0041T, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LAMP-1/CD107a

Lysosome-associated membrane protein-1 (LAMP1), also known as CD107a, is a 100‑130 kDa member of the LAMP family of glycoproteins. It is expressed in lysosomal and plasma membranes of macrophages, NK and T-cells, and with LAMP2, is essential for the formation of phagolysosomes. On the cell surface, it also presents carbohydrates to selectins. Mature human LAMP1 is a 389 amino acid (aa) type I transmembrane glycoprotein. It contains a 354 aa luminal/extracellular domain (ECD) (aa 28‑381) and a 12 aa cytoplasmic tail (aa 405‑416). The ECD has two large looping regions (aa 28‑193 and 227‑381) plus multiple N- and O-linked glycosylation sites. There is one potential splice variant that shows a 26 aa substitution in the signal sequence. Over aa 28‑380, human LAMP1 shares 64% aa identity with mouse LAMP1.

  • Long Name:
    Lysosome-associated Membrane Glycoprotein 1
  • Entrez Gene IDs:
    3916 (Human); 16783 (Mouse)
  • Alternate Names:
    CD107 antigen-like family member A; CD107a antigen; CD107a; LAMP1; LAMP-1; LAMPA; LGP120; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1
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