Human LAMP-1/CD107a Alexa Fluor® 594-conjugated Antibody Summary
Accession # P11279
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of LAMP-1/CD107a in THP‑1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human LAMP-1/CD107a Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # IC4800T, filled histogram) or isotype control antibody (Catalog # IC0041T, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Lysosome-associated membrane protein-1 (LAMP1), also known as CD107a, is a 100‑130 kDa member of the LAMP family of glycoproteins. It is expressed in lysosomal and plasma membranes of macrophages, NK and T-cells, and with LAMP2, is essential for the formation of phagolysosomes. On the cell surface, it also presents carbohydrates to selectins. Mature human LAMP1 is a 389 amino acid (aa) type I transmembrane glycoprotein. It contains a 354 aa luminal/extracellular domain (ECD) (aa 28‑381) and a 12 aa cytoplasmic tail (aa 405‑416). The ECD has two large looping regions (aa 28‑193 and 227‑381) plus multiple N- and O-linked glycosylation sites. There is one potential splice variant that shows a 26 aa substitution in the signal sequence. Over aa 28‑380, human LAMP1 shares 64% aa identity with mouse LAMP1.
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