|Detection of LAMP‑1/CD107a in Human NK cells by Flow Cytometry. Human NK Cells either (A) stimulated ina 1:1 ratio with K562 human chronic myelogenous leukemia cell line and 3 μM monensin for 5 hours or (B) unstimulated were stained with Mouse Anti-Human LAMP‑1/CD107a APC‑conjugated Monoclonal Antibody (Catalog # IC4800A) and Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.|
Intracellular Staining by Flow Cytometry
|Detection of LAMP-1/CD107a in THP‑1 Human Cell Line by Flow Cytometry. THP‑1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human LAMP-1/CD107a APC‑conjugated Monoclonal Antibody (Catalog # IC4800A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.|
Lysosome-Associated Membrane Protein-1 (LAMP-1), also known as CD107a, is a 100-130 kDa member of the LAMP family of glycoproteins. It is expressed in lysosomal and plasma membranes of macrophages, NK and T-cells, and with LAMP-2, is essential for the formation of phagolysosomes. On the cell surface, it also presents carbohydrates to selectins. Mature human LAMP-1 is a 389 amino acid (aa) type I transmembrane glycoprotein. It contains a 354 aa luminal/extracellular domain (ECD) (aa 28-381) and a 12 aa cytoplasmic tail (aa 405-416). The ECD has two large looping regions (aa 28-193 and 227-381) plus multiple N- and O-linked glycosylation sites. There is one potential splice variant that shows a 26 aa substitution in the signal sequence. Over aa 28-380, human LAMP-1 shares 64% aa identity with mouse LAMP-1.