Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry, ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 508921
View all LAMP-1/CD107a Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LAMP1/CD107a
Ala28-Asn380
Accession # P11279

Specificity

Detects human LAMP1/CD107a in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human LAMP‑1/CD107a Antibody

LAMP1/CD107a antibody in Human Kidney by Immunohistochemistry (IHC-P).

LAMP1/CD107a in Human Kidney.

LAMP1/CD107a was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human LAMP1/CD107a Monoclonal Antibody (Catalog # MAB4800) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to lysosomes in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
LAMP-1/CD107a antibody in THP-1 Human Cell Line by Immunocytochemistry (ICC).

LAMP‑1/CD107a in THP‑1 Human Cell Line.

LAMP-1/CD107a was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Mouse Anti-Human LAMP-1/CD107a Monoclonal Antibody (Catalog # MAB4800) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI(blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human LAMP-1/CD107a by Western Blot

Detection of Human LAMP-1/CD107a by Western Blot

Increased SNX9 expression and co-localization with podocin are detectable in the cytoplasm of ADR-treated WT podocytes, whereas SNX9 KD podocytes exhibit little cytoplasmic expression of podocin.(a) Fluorescent micrographs of cultured human podocytes stained with SNX9 (green) and podocin (red) before and after ADR treatment (merged areas are in yellow). DAPI (blue) was used to indicate nuclei. Boxes indicate higher magnification areas presented in the lower panels. (b) Western blot analyses of the fractions from control podocytes or podocytes treated with ADR separated on linear OptiPrep gradients (5–25%). Distributions of SNX9 and podocin, as well as marker proteins of plasma membrane (caveolin), endosome/lysosome (LAMP1), mitochondria ( beta subunit of F1F0-ATPase), and endoplasmic reticulum (calnexin), were examined by western blot analysis. (c) Cultured human podocytes were transfected with nonfunctional control siRNA (upper panel) or SNX9 siRNA (middle and lower panels). Transfected cells, as decided by GFP expression, are indicated by arrowheads. Upper panel: Fluorescent micrographs of control siRNA-transfected podocyte stained with SNX9 (red). Middle panel: Fluorescent micrographs of SNX9 siRNA-transfected podocyte with ADR treatment stained with SNX9 (red). Lower panel: Fluorescent micrographs of SNX9 siRNA-transfected podocyte with ADR treatment stained with podocin (red). DAPI (blue) was used to indicate nuclei. Boxes indicate higher-magnification areas presented on the right. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28266622), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human LAMP‑1/CD107a Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed THP-1 human acute monocytic leukemia cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: THP‑1 human acute monocytic leukemia cell line fixed with paraformaldehyde and permeabilized with saponin

Reviewed Applications

Read 1 review rated 5 using MAB4800 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LAMP-1/CD107a

Lysosome-associated membrane protein-1 (LAMP1), also known as CD107a, is a 100‑130 kDa member of the LAMP family of glycoproteins. It is expressed in lysosomal and plasma membranes of macrophages, NK and T-cells, and with LAMP2, is essential for the formation of phagolysosomes. On the cell surface, it also presents carbohydrates to selectins. Mature human LAMP1 is a 389 amino acid (aa) type I transmembrane glycoprotein. It contains a 354 aa luminal/extracellular domain (ECD) (aa 28‑381) and a 12 aa cytoplasmic tail (aa 405‑416). The ECD has two large looping regions (aa 28‑193 and 227‑381) plus multiple N- and O-linked glycosylation sites. There is one potential splice variant that shows a 26 aa substitution in the signal sequence. Over aa 28‑380, human LAMP1 shares 64% aa identity with mouse LAMP1.

Long Name

Lysosome-associated Membrane Glycoprotein 1

Alternate Names

CD107a, LAMP1

Entrez Gene IDs

3916 (Human); 16783 (Mouse)

Gene Symbol

LAMP1

UniProt

P11279

Additional LAMP-1/CD107a Products

Product Documents for Human LAMP‑1/CD107a Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LAMP‑1/CD107a Antibody

For research use only

Citations for Human LAMP‑1/CD107a Antibody

Customer Reviews for Human LAMP‑1/CD107a Antibody (1)

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  • Human LAMP-1/CD107a Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: THP‑1 Human Cell Line
    Species: Human
    Verified Customer | Posted 02/10/2022
    Human LAMP‑1/CD107a Antibody MAB4800

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