Designed to visualize the expression of LAMP-1 by fluorescence microscopy for staining cells and tissues. Conjugated LAMP-1 antibodies are ideal for immunocytochemistry colocalization studies in lysosomes. The unconjugated antibody detects human LAMP-1/CD107a in direct ELISAs and Western blots.
Monoclonal Mouse IgG2B Clone # 508921
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human LAMP-1/CD107a Ala28-Asn380 Accession # P11279
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of LAMP‑1/CD107a in Human NK cells by Flow Cytometry.
Human NK Cells either (A) stimulated in a 1:1 ratio with K562 human chronic myelogenous leukemia cell line and 3 μM monensin for 5 hours or (B) ustimulated were stained with Mouse Anti-Human LAMP‑1/CD107a Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC4800G) and Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on control antibody staining (Catalog # IC0041G). View our protocol for Staining Membrane-associated Proteins.
LAMP1/CD107a in HeLa Human Cell Line.
LAMP1/CD107a was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human LAMP1/CD107a Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC4800G) at 1:10 dilution for 3 hours at room temperature and counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Lysosome-associated membrane protein-1 (LAMP-1), also known as CD107a, is a 100‑130 kDa member of the LAMP family of glycoproteins. It is expressed in lysosomal and plasma membranes of macrophages, NK and T-cells, and with LAMP-2, is essential for the formation of phagolysosomes. On the cell surface, it also presents carbohydrates to selectins. Mature human LAMP-1 is a 389 amino acid (aa) type I transmembrane glycoprotein. It contains a 354 aa luminal/extracellular domain (ECD) (aa 28‑381) and a 12 aa cytoplasmic tail (aa 405‑416). The ECD has two large looping regions (aa 28‑193 and 227‑381) plus multiple N- and O-linked glycosylation sites. There is one potential splice variant that shows a 26 aa substitution in the signal sequence. Over aa 28‑380, human LAMP-1 shares 64% aa identity with mouse LAMP-1.
Lysosome-associated Membrane Glycoprotein 1
Entrez Gene IDs:
3916 (Human); 16783 (Mouse)
CD107 antigen-like family member A; CD107a antigen; CD107a; LAMP1; LAMP-1; LAMPA; LGP120; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1
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