Human LAP (TGF-beta 1) PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2463P
Detection of LAP (TGF-beta 1) in Human Platelets by Flow Cytometry.
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Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (1)

Human LAP (TGF-beta 1) PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human LAP (TGF-beta 1) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) TGF‑ beta 1, rhTGF‑ beta 2, rhTGF‑ beta 1.2, rhTGF‑ beta 3, or rhTGF‑ alpha.
Source
Monoclonal Mouse IgG1 Clone # 27232
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP (TGF-beta 1)
Leu30-Arg278 (Cys33Ser)
Accession # P01137
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of LAP (TGF-beta 1) in Human Platelets by Flow Cytometry. View Larger

Detection of LAP (TGF-beta 1) in Human Platelets by Flow Cytometry. Human CD41+platelets were stained with Mouse Anti-Human LAP (TGF-beta 1) PE-conjugated Monoclonal Antibody (Catalog # FAB2463P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: LAP (TGF-beta 1)

The TGF‑ beta family includes several related proteins (70‑80% sequence homology) from mammalian, avian, or Xenopus systems that are now designated TGF‑ beta 1, TGF‑ beta 2, TGF‑ beta 1.2, TGF‑ beta 3, TGF‑ beta 4, and TGF‑ beta 5. These proteins are secreted by cells in the form of an inactive complex, referred to as latent TGF-beta, that consists of TGF‑ beta associated non-covalently with a Latency-associated peptide (LAP). These two proteins are synthesized as a single pro-peptide that is cleaved in a post Golgi compartment prior to secretion. Different TGF‑ beta family members are naturally associated with their own distinct LAPs.

Long Name
Latency-associated Peptide
Entrez Gene IDs
7040 (Human)
Alternate Names
CED; DPD1; LAP (TGFbeta 1); LAP (TGF-beta 1); LAP; TGFB; TGFB1; TGFbeta; transforming growth factor beta 1

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Citations for Human LAP (TGF-beta 1) PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Suppressive regulatory T cells and latent transforming growth factor-?-expressing macrophages are altered in the peritoneal fluid of patients with endometriosis
    Authors: T Hanada, S Tsuji, M Nakayama, S Wakinoue, K Kasahara, F Kimura, T Mori, K Ogasawara, T Murakami
    Reprod. Biol. Endocrinol., 2018;16(1):9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. TGF-beta/atRA-induced Tregs express a selected set of microRNAs involved in the repression of transcripts related to Th17 differentiation
    Authors: JLDS Schiavinat, R Haddad, F Saldanha-A, J Baiochi, AG Araujo, P Santos Sch, DT Covas, MA Zago, RA Panepucci
    Sci Rep, 2017;7(1):3627.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Narcolepsy Type 1 Is Associated with a Systemic Increase and Activation of Regulatory T Cells and with a Systemic Activation of Global T Cells
    Authors: M Lecendreux, G Churlaud, F Pitoiset, A Regnault, TA Tran, R Liblau, D Klatzmann, M Rosenzwajg
    PLoS ONE, 2017;12(1):e0169836.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. A Cellular MicroRNA Facilitates Regulatory T Lymphocyte Development by Targeting the FOXP3 Promoter TATA-Box Motif
    Authors: Y Zhang, W Liu, Y Chen, J Liu, K Wu, L Su, W Zhang, Y Jiang, X Zhang, Y Zhang, C Liu, L Tao, B Liu, H Zhang
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Human Regulatory T Cells Mediate Transcriptional Modulation of Dendritic Cell Function
    Authors: Emily Mavin
    J. Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Impaired circulating CD4+ LAP+ regulatory T cells in patients with acute coronary syndrome and its mechanistic study.
    Authors: Zhu Z, Meng K, Zhong Y, Qi L, Mao X, Yu K, Zhang W, Zhu P, Ren Z, Wu B, Ji Q, Wang X, Zeng Q
    PLoS ONE, 2014;9(2):e88775.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Steady state migratory RelB+ langerin+ dermal dendritic cells mediate peripheral induction of antigen-specific CD4+ CD25+ Foxp3+ regulatory T cells.
    Authors: Azukizawa H, Dohler A, Kanazawa N, Nayak A, Lipp M, Malissen B, Autenrieth I, Katayama I, Riemann M, Weih F, Berberich-Siebelt F, Lutz MB
    Eur. J. Immunol., 2011;41(5):1420-34.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut.
    Authors: Suzuki K, Maruya M, Kawamoto S
    Immunity, 2010;33(1):71-83.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. A crucial role for Kupffer cell-derived galectin-9 in regulation of T cell immunity in hepatitis C infection.
    Authors: Mengshol JA, Golden-Mason L, Arikawa T
    PLoS ONE, 2010;5(3):e9504.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Peptide inhibitors of transforming growth factor-beta enhance the efficacy of antitumor immunotherapy.
    Authors: Llopiz D, Dotor J, Casares N, Bezunartea J, Diaz-Valdes N, Ruiz M, Aranda F, Berraondo P, Prieto J, Lasarte JJ, Borras-Cuesta F, Sarobe P
    Int. J. Cancer, 2009;125(11):2614-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

  1. How do you isolate human platelets for flow cytometry, using Human LAP (TGF-beta 1) Antibody, Catalog # MAB2463, and its conjugates? 

    • We obtain the platelets from blood plasma by collection the top layer after spinning down whole blood. We proceed with a normal surface stain protocol; no special buffers are required. 

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Reviews for Human LAP (TGF-beta 1) PE-conjugated Antibody

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Human LAP (TGF-beta 1) PE-conjugated Antibody
By Anonymous on 06/24/2018
Application: the antibody worked well. Sample Tested: CD4 T cells,CD8 T cells Species: Human