Detection of Human Latrophilin 3/LPHN3 by Western Blot.
Western blot shows lysates of human cerebellum tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Latrophilin 3/ LPHN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5916) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Latrophilin 3/ LPHN3 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Latrophilin 3/LPHN3
LPHN3 (Latrophilin 3; also CIRL3, CL3 and LEC3) is a predicted 162 kDa member of the LN-TM7 subfamily, GPCR 2 family of molecules. It appears to have a restricted expression pattern, being limited to the brain and adrenal gland. Although it is related to the black widow toxin receptor CIRL1, it does not serve as a toxin receptor. Mature human LPHN3 is a 7‑TM glycoprotein that is 1428 amino acids (aa) in length. Posttranslational processing cleaves the molecule into a 120 kDa ECD (aa 20‑841) and a noncovalently-associated 85 kDa 7-TM C-terminus (aa 842‑1447). The ECD is modular, and contains a SUEL-like lectin domain (aa 35‑124), an Olf region (aa 134‑393) and GPS domain (aa 802‑853). There are multiple splice events which, in the ECD, include a two aa substitution for aa 127‑131, a 13 aa insertion after Lys623, and a 39 aa substitution for aa 668‑1447. Over aa 20‑813, human LPHN3 shares 98% aa identity with mouse LPHN3.
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