Human Latrophilin 3/LPHN3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Latrophilin 3/LPHN3 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Latrophilin 3/LPHN3
    Phe20-Asn813
    Accession # NP_056051
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Latrophilin 3/LPHN3 by Western Blot. Western blot shows lysates of human cerebellum tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Latrophilin 3/
LPHN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5916) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Latrophilin 3/
LPHN3 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Latrophilin 3/LPHN3
LPHN3 (Latrophilin 3; also CIRL3, CL3 and LEC3) is a predicted 162 kDa member of the LN-TM7 subfamily, GPCR 2 family of molecules. It appears to have a restricted expression pattern, being limited to the brain and adrenal gland. Although it is related to the black widow toxin receptor CIRL1, it does not serve as a toxin receptor. Mature human LPHN3 is a 7‑TM glycoprotein that is 1428 amino acids (aa) in length. Posttranslational processing cleaves the molecule into a 120 kDa ECD (aa 20‑841) and a noncovalently-associated 85 kDa 7-TM C-terminus (aa 842‑1447). The ECD is modular, and contains a SUEL-like lectin domain (aa 35‑124), an Olf region (aa 134‑393) and GPS domain (aa 802‑853). There are multiple splice events which, in the ECD, include a two aa substitution for aa 127‑131, a 13 aa insertion after Lys623, and a 39 aa substitution for aa 668‑1447. Over aa 20‑813, human LPHN3 shares 98% aa identity with mouse LPHN3.
  • Entrez Gene IDs:
    23284 (Human); 319387 (Mouse); 170641 (Rat)
  • Alternate Names:
    CIRL3; Latrophilin 3; latrophilin homolog 3 (cow); LEC3; Lectomedin-3; LPHN3
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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